Vegetos
(An International Journal of Plant Research & Biotechnology)
Impact of strong correlations observed in sugarcane responses to sodium chloride and mannitol within temporary immersion bioreactors
*Article not assigned to an issue yet
Lozada Alberto, Gómez Daviel, Acosta Yanier, Quintana-Zaez Julio César, Companioni Barbarita, Zevallos–Bravo Byron E., de Lourdes Tapia y Figueroa María, Sershen, Lorenzo José Carlos, Gómez Daviel
Short Communications | Published: 11 June, 2025
First Page: 0
Last Page: 0
Views: 541
Keywords: n Abiotic stressn , Drought, In vitro selection, n Saccharum officinarum L., Salinity
Abstract
Sugarcane is a vital crop in tropical and subtropical regions worldwide, contributing to economic growth, energy production, and nutritional value. Over the past decade, numerous studies have demonstrated that in vitro screening for stress tolerance enables rapid evaluation of many plants, propagules, and explants for desirable traits, significantly accelerating the breeding process compared to traditional field methods. This report showcases the correlation between responses to NaCl (salinity) and mannitol (drought) in sugarcane plantlets micropropagated in temporary immersion bioreactors. With coefficients of determination (R2) exceeding 90%, the strongest correlations between sugarcane responses to salinity and drought were observed in the following indicators: shoot multiplication rate (90%), fresh weight of shoot clusters (97%), and levels of malondialdehydes (95%), other aldehydes (98%), carotenoids (95%), and soluble phenolics in the plantlets (96%). Soluble phenolics excreted into the culture medium showed an R2 of 86%, and chlorophyll a had an R2 of 78%. In contrast, chlorophyll b levels and cell wall-linked phenolics exhibited very low coefficients of determination (less than 25%). These findings highlight the strong physiological and biochemical responses of sugarcane to salinity and drought, particularly through phenolic metabolism and oxidative stress markers. The high coefficients of determination indicate reliable predictive relationships, suggesting that these indicators can serve as valuable biomarkers for stress tolerance. In contrast, the weak correlations observed in chlorophyll b and cell wall-linked phenolics underscore their limited relevance in assessing sugarcane resilience under these conditions.
References
Aferri G, da Costa CC, de Lima CG, de Oliveira MMdL, Batista K, Barbosa CMP (2024) Technical, economic and social dimensions of sugarcane (Saccharum officinarum) in animal feed: a study with the application of multivariate data analysis. Rev Gest Secr 15:1–18
Ait Barka E, Kalantari S, Makhlouf J, Arul J (2000) Effects of UV-C irradiation on lipid peroxidation markers during ripening of tomato (Lycopersicon esculentum L.) fruits. Funct Plant Biol 27:147–152
Anwar A, Kim J-K (2020) Transgenic breeding approaches for improving abiotic stress tolerance: recent progress and future perspectives. Int J Mol Sci 21:1–29
Chandel N, Kumar A, Kumar R (2024) Towards sustainable agriculture: integrating agronomic practices, environmental physiology and plant nutrition. Int J Plant Soil Sci 36:492–503
Cominelli E, Conti L, Tonelli C, Galbiati M (2013) Challenges and perspectives to improve crop drought and salinity tolerance. New Biotechnol 30:355–361
Corwin DL (2021) Climate change impacts on soil salinity in agricultural areas. Eur J Soil Sci 72:842–862
Dos Santos TB, Ribas AF, de Souza SGH, Budzinski IGF, Domingues DS (2022) Physiological responses to drought, salinity, and heat stress in plants: a review. Stresses 2:113–135
Gavrilescu M (2021) Water, soil, and plants interactions in a threatened environment. Water 13:2746
Gómez D, Hernández L, Valle B, Martínez J, Cid M, Escalona M, Hernández M, Yabor L, Beemster GTS, Tebbe CC, Papenbrock J, Lorenzo JC (2017) Salinity induces specific metabolic changes in sugarcane shoot explants in temporary immersion bioreactors. J Appl Biol Food Qual 90:354–358
Gómez D, Hernández L, Yabor L, Beemster GTS, Tebbe CC, Papenbrock J, Lorenzo JC (2018) Euclidean distance can identify the mannitol level that produces the most remarkable integral effect on sugarcane micropropagation in temporary immersion bioreactors. J Plant Res 131:719–724
Gonçalves MC, Pinto LR, Pimenta RJG, da Silva MF (2024) Sugarcane. Viral diseases of field and horticultural crops. Elsevier, Amsterdam, pp 193–205
Gonzalez Guzman M, Cellini F, Fotopoulos V, Balestrini R, Arbona V (2022) New approaches to improve crop tolerance to biotic and abiotic stresses. Physiol Plant 174:e13547
Gurr S, McPherson J, Bowles D (1992) Lignin and associated phenolic acids in cell walls. In: Wilkinson DL (ed) Molecular plant pathology. Oxford Press, Oxford, p 62
Han L, Zhang Q, Zhang Z, Jia J, Wang Y, Huang T, Cheng Y (2021) Drought area, intensity and frequency changes in China under climate warming, 1961–2014. J Arid Environ 193:104596
Heath R, Packer J (1968) Photoperoxidation in isolated chloroplast: I. Kinetics and stoichiometry of fatty acid peroxidation. Arch Biochem Biophys 125:189–198
Hoagland RE (1990) Alternaria cassiae alters phenylpropanoid metabolism in Sicklepod (Casia obstusifolia). J Phytopathol 130:177–187
Hopmans JW, Qureshi A, Kisekka I, Munns R, Grattan S, Rengasamy P, Ben-Gal A, Assouline S, Javaux M, Minhas P (2021) Critical knowledge gaps and research priorities in global soil salinity. Adv Agron 169:1–191
Jiménez E, Pérez J, Gil V, Herrera J, García Y, Alonso E (1995) Sistema para la propagación de la caña de azúcar. In: Estrade M, Riego E, Limonta E, Tellez P, Fuente J (eds) Avances en biotecnología moderna. Elfos Scientiae, La Habana, p 112
Kachare NV, Khan A, Suryawanshi MV (2023) Impact of climate change on sugarcane production and its mitigation a review. Int J Prog Res Eng Manag Sci 3:389–396
Kumar S (2020) Abiotic stresses and their effects on plant growth, yield and nutritional quality of agricultural produce. Int J Food Sci Agric 4:367–378
Lesk C, Anderson W, Rigden A, Coast O, Jägermeyr J, McDermid S, Davis KF, Konar M (2022) Compound heat and moisture extreme impacts on global crop yields under climate change. Nat Rev Earth Environ 3:872–889
Lichtenthaler HK (1987) Chlorophylls and carotenoids: pigments of photosynthetic biomembranes. Meth Enzymol 148:350–382
Lorenzo JC, González B, Escalona M, Teisson C, Espinosa P, Borroto C (1998) Sugarcane shoot formation in an improved temporary immersion system. Plant Cell Tiss Org Cult 54:197–200. https://doi.org/10.1023/A:1006168700556
Lottering S, Mafongoya P, Lottering R (2021) Drought and its impacts on small-scale farmers in sub-Saharan Africa: a review. South Afric Geog J 103:319–341
Ma Y, Dias MC, Freitas H (2020) Drought and salinity stress responses and microbe-induced tolerance in plants. Front Plant Sci 11:591911
Machimu GM (2024) Next steps for smallholder sugarcane contract farmers in developing countries: a review. Soc Sci Hum Op 9:1–9
Matlou R, Bahta YT, Owusu-Sekyere E, Jordaan H (2021) Impact of agricultural drought resilience on the welfare of smallholder livestock farming households in the northern Cape province of South Africa. Land 10:562
Mishra N, Tripathi MK, Tiwari S, Tripathi N, Sapre S, Ahuja A, Tiwari S (2021) Cell suspension culture and in vitro screening for drought tolerance in soybean using poly-ethylene glycol. Plants 10:517
Okur B, Örçen N (2020) Soil salinization and climate change. In: Vara Prasad MN, Pietrzykowski M (eds) Climate change and soil interactions. Elsevier, London, pp 331–350
Piekutowska M, Niedbała G, Piskier T, Lenartowicz T, Pilarski K, Wojciechowski T, Pilarska AA, Czechowska-Kosacka A (2021) The application of multiple linear regression and artificial neural network models for yield prediction of very early potato cultivars before harvest. Agronomy 11:885
Porra R (2002) The chequered history of the development and use of simultaneous equations for the accurate determination of chlorophylls a and b. Photosynth Res 73:149–156
Samantara K, Bohra A, Mohapatra SR, Prihatini R, Asibe F, Singh L, Reyes VP, Tiwari A, Maurya AK, Croser JS (2022) Breeding more crops in less time: a perspective on speed breeding. Biology 11:275
Seleiman MF, Al-Suhaibani N, Ali N, Akmal M, Alotaibi M, Refay Y, Dindaroglu T, Abdul-Wajid HH, Battaglia ML (2021) Drought stress impacts on plants and different approaches to alleviate its adverse effects. Plants 10:1–25
Solomon S, Vanichsriratana W, Duong CA, Manimekalai R, Singh P, Misra V, Rao GP (2024) Sustainability issues and opportunities for sugar and integrated industries in ASEAN Region. Sug Tech 26:932–950
Temesgen T, Periyasamy S, Mensur D, Berhane B, Sunaina JM (2024) Valorization of wastes and by-products of cane-based sugar industry. In: Cherian E, Gurunathan B (eds) Value added products from food waste. Springer, Cham, pp 185–204
Tripathy KP, Mukherjee S, Mishra AK, Mann ME, Williams AP (2023) Climate change will accelerate the high-end risk of compound drought and heatwave events. Proc Natl Acad Sci 120:e2219825120
Author Information
Department for Plant Breeding and Conservation of Genetic Resources, Bioplant Center, University of Ciego de Ávila Máximo Gómez Báez, Modesto Reyes, Cuba