Vegetos
(An International Journal of Plant Research & Biotechnology)
Evaluate the efficacy of guava-derived CuO, and ZnO nanocomposite coating on shelf life of grapes
*Article not assigned to an issue yet
Singh Neha, Mittal Avneesh, Garg Meenakshi, Trivedi Rishabh, Kumar Pola Pavan, Sadhu Susmita Dey, Chopra Rajni
Research Articles | Published: 28 May, 2025
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Keywords: Nanocomposite coating, Biopolymer, Pectin, Grapes, Shelf life, Sustainable packaging
Abstract
Pectin’s anionic heteropolysaccharide structure makes it suitable for coating metal nanoparticles (MPNs) by preventing their accumulation, and improves MPNs’ acceptability to formulate edible coatings. The present investigation explored the efficacy of guava-derived pectin-based zinc oxide (ZnO) and copper oxide (CuO) nanocomposite biopolymer coatings on the quality attributes and shelf-life extension of berries. The solvent casting and chemical reduction methods were used to synthesize guava-derived ZnO and CuO nanoparticles coatings, respectively. Simultaneously, berries were submerged in three different biopolymer coatings, viz., guava-derived pectin (GDP), GDP/ZnO, and GDP/CuO for three minutes and stored at refrigerated temperature (i.e., 7 °C) along with uncoated samples. The samples were evaluated for physicochemical changes and sensorial parameters at an interval of 2 days for a 19-day storage period and FTIR spectroscopy. The nanocomposite coatings were effective in reducing the berries’ weight loss, water activity, and minimizing the reduction in pH and titratable acidity. Comparably, an increase in total soluble solids (TSS) was observed in all coated samples, but it was lower than uncoated ones. Furthermore, compared to controlled and other coated samples, only ZnO-coated berries retained their texture, odor, and were of acceptable quality to a lesser extent until 19 days of storage. The results of FTIR spectroscopy reveal the presence of alcohol and carboxylic acid groups in the GDP structure. Antibacterial assay showed that ZnO nanoparticles showed a stronger zone of inhibition than CuO nanoparticles, thereby aiding in improving the shelf life of grapes. Supposedly, nanocomposite coatings could be harnessed as an active biopolymer packaging material that can prolong the lifespan and maintain the quality of grapes for an adequate period (at least two weeks) under refrigerated conditions.
References
Amiri S, Rezazad Bari L, Ahmadi Partovi G (2022) The effect of edible coating based on aloe vera gel containing copper oxide nanoparticles on fresh-cut Kiwi fruit slices at refrigerator temperature. Packag Technol Sci 13
Ayala-Zavala JF, Silva‐Espinoza BA, Cruz‐Valenzuela MR et al (2013) Pectin–cinnamon leaf oil coatings add antioxidant and antibacterial properties to fresh‐cut Peach. Flavour Fragr J 28:39–45. https://doi.org/10.1002/ffj.3125
Ayoubi A, Balvardi M, Mahmoudi-kordi F (2022) Maintaining the nutritional quality and increasing the shelf life of dried apricot using sodium alginate and pectin as edible coating. J Food Meas Charact 16:4025–4035. https://doi.org/10.1007/s11694-022-01508-w
Černá M, Barros AS, Nunes A et al (2003) Use of FT-IR spectroscopy as a tool for the analysis of polysaccharide food additives. Carbohydr Polym 51:383–389. https://doi.org/10.1016/S0144-8617(02)00259-X
Chavez-Esquivel G, Ángeles-Beltrán D, de la Torre PMT et al (2025) Antimicrobial and antifungal edible coatings with ZnO nanoparticles dispersed in a chitosan-guar gum matrix for Hass avocado preservation. Int J Biol Macromol 308:142467. https://doi.org/10.1016/j.ijbiomac.2025.142467
Dadi R, Azouani R, Traore M et al (2019) Antibacterial activity of ZnO and CuO nanoparticles against gram positive and gram negative strains. Mater Sci Engineering: C 104:109968. https://doi.org/10.1016/j.msec.2019.109968
Dakal TC, Kumar A, Majumdar RS, Yadav V (2016) Mechanistic basis of antimicrobial actions of silver nanoparticles. Front Microbiol 7. https://doi.org/10.3389/fmicb.2016.01831
Esmailzadeh H, Sangpour P, Shahraz F et al (2021) CuO/LDPE nanocomposite for active food packaging application: a comparative study of its antibacterial activities with ZnO/LDPE nanocomposite. Polym Bull 78:1671–1682. https://doi.org/10.1007/s00289-020-03175-7
Foo SY, Nur Hanani ZA, Rozzamri A et al (2019) Effect of Chitosan–Beeswax edible coatings on the Shelf-life of Sapodilla (Achras zapota) fruit. J Packag Technol Res 3:27–34. https://doi.org/10.1007/s41783-018-0047-0
García MA, Martino MN, Zaritzky NE (1998) Plasticized Starch-Based coatings to improve strawberry (Fragaria × Ananassa) quality and stability. J Agric Food Chem 46:3758–3767. https://doi.org/10.1021/jf980014c
Guerreiro AC, Gago CML, Faleiro ML et al (2017) The effect of edible coatings on the nutritional quality of ‘Bravo de Esmolfe’ fresh-cut Apple through shelf-life. LWT 75:210–219. https://doi.org/10.1016/j.lwt.2016.08.052
Isopencu GO, Stoica-Guzun A, Busuioc C et al (2021) Development of antioxidant and antimicrobial edible coatings incorporating bacterial cellulose, pectin, and blackberry pomace. Carbohydr Polym Technol Appl 2:100057. https://doi.org/10.1016/j.carpta.2021.100057
Khairy T, Amin DH, Salama HM et al (2024) Antibacterial activity of green synthesized copper oxide nanoparticles against multidrug-resistant bacteria. Sci Rep 14:25020. https://doi.org/10.1038/s41598-024-75147-0
Kim I, Viswanathan K, Kasi G et al (2022) ZnO nanostructures in active antibacterial food packaging: Preparation methods, antimicrobial mechanisms, safety issues, future prospects, and challenges. Food Reviews Int 38:537–565. https://doi.org/10.1080/87559129.2020.1737709
Kumar L, Ramakanth D, Akhila K, Gaikwad KK (2022) Edible films and coatings for food packaging applications: a review. Environ Chem Lett 20:875–900. https://doi.org/10.1007/s10311-021-01339-z
Kumar S, Boro JC, Ray D et al (2019) Bionanocomposite films of agar incorporated with ZnO nanoparticles as an active packaging material for shelf life extension of green grape. Heliyon 5:e01867. https://doi.org/10.1016/j.heliyon.2019.e01867
Mendes CR, Dilarri G, Forsan CF et al (2022) Antibacterial action and target mechanisms of zinc oxide nanoparticles against bacterial pathogens. Sci Rep 12:2658. https://doi.org/10.1038/s41598-022-06657-y
Moalemiyan M, Ramaswamy HS, Maftoonazad N, Pectin-based edible coating for shelf-life extension of Ataulfo mango (2012) J Food Process Eng 35:572–600. https://doi.org/10.1111/j.1745-4530.2010.00609.x
Mohd Suhaimi NI, Mat Ropi AA, Shaharuddin S (2021) Safety and quality preservation of starfruit (Averrhoa carambola) at ambient shelf life using synergistic pectin-maltodextrin-sodium chloride edible coating. Heliyon 7:e06279. https://doi.org/10.1016/j.heliyon.2021.e06279
Muñoz-Escobar A, Reyes-López SY (2020) Antifungal susceptibility of Candida species to copper oxide nanoparticles on Polycaprolactone fibers (PCL-CuONPs). PLoS ONE 15:e0228864. https://doi.org/10.1371/journal.pone.0228864
Nawab A, Alam F, Hasnain A (2017) Mango kernel starch as a novel edible coating for enhancing shelf- life of tomato (Solanum lycopersicum) fruit. Int J Biol Macromol 103:581–586. https://doi.org/10.1016/j.ijbiomac.2017.05.057
Nemiwal M, Zhang TC, Kumar D (2021) Pectin modified metal nanoparticles and their application in property modification of biosensors. Carbohydr Polym Technol Appl 2:100164. https://doi.org/10.1016/j.carpta.2021.100164
Panahirad S, Dadpour M, Peighambardoust SH et al (2021) Applications of carboxymethyl cellulose- and pectin-based active edible coatings in preservation of fruits and vegetables: A review. Trends Food Sci Technol 110:663–673. https://doi.org/10.1016/j.tifs.2021.02.025
Qi PX, Chau HK, Fishman ML et al (2017) Investigation of the molecular interactions between β-lactoglobulin and low methoxyl pectin by multi-detection high performance size exclusion chromatography. Food Hydrocoll 63:321–331. https://doi.org/10.1016/j.foodhyd.2016.09.016
Rajendrachari S, Kamacı Y, Taş R et al (2019) Antimicrobial investigation of CuO and ZnO nanoparticles prepared by a rapid combustion method. Phys Chem Res 7:799–812
Ramazanzadeh B, Jahanbin A, Yaghoubi M et al (2015) Comparison of antibacterial effects of ZnO and CuO nanoparticles coated brackets against Streptococcus Mutans. J Dent (Shiraz) 16:200–205
Sabir FK, Sabir A, Unal S et al (2019) Postharvest quality extension of minimally processed table grapes by Chitosan coating. Int J Fruit Sci 19:347–358. https://doi.org/10.1080/15538362.2018.1506961
Sanchís E, González S, Ghidelli C et al (2016) Browning Inhibition and microbial control in fresh-cut persimmon (Diospyros Kaki thunb. Cv. Rojo Brillante) by Apple pectin-based edible coatings. Postharvest Biol Technol 112:186–193. https://doi.org/10.1016/j.postharvbio.2015.09.024
Sharma B, Nigam S, Verma A et al (2023) A biogenic approach to develop guava derived edible copper and zinc oxide Nanocoating to extend shelf life and efficiency for food preservation. J Polym Environ. https://doi.org/10.1007/s10924-023-02972-1
Sun C, Cao J, Wang Y et al (2022) Preparation and characterization of pectin-based edible coating agent encapsulating carvacrol/HPβCD inclusion complex for inhibiting fungi. Food Hydrocoll 125:107374. https://doi.org/10.1016/j.foodhyd.2021.107374
Wardana AA, Kingwascharapong P, Tanaka F, Tanaka F (2021) CuO Nanoparticles/Indonesian Cedarwood essential oil-loaded Chitosan coating film: characterisation and antifungal improvement against Penicillium spp. Int J Food Sci Technol 56:4224–4238. https://doi.org/10.1111/ijfs.15195
Xu W, Li D, Fu Y et al (2013) Extending the shelf life of Victoria table grapes by high permeability and fungicide packaging at room temperature. Packaging Technol Sci 26:43–50. https://doi.org/10.1002/pts.2002
Yaman Ö, Bayoιndιrlι L (2002) Effects of an edible coating and cold storage on Shelf-life and quality of cherries. LWT - Food Sci Technol 35:146–150. https://doi.org/10.1006/fstl.2001.0827
Author Information
Bhaskaracharya College of Applied Science, University of Delhi, New Delhi, India