Vegetos
(An International Journal of Plant Research & Biotechnology)
Antifungal potential of seaweed polysaccharide and its nanoparticles against soil borne pathogens of tomato
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Research Articles | Published: 10 June, 2025
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Keywords: n Gracillaria salicornian , Sulphated galactan, Hydrocolloid, Soil borne phytopathogen, Inhibition, Nanobiocide
Abstract
The aim of the present study was to prepare an eco-friendly alternative compound to use as a nanobiocide to control phytopathogens of tomato. In this study, Polysaccharides were isolated from Gracillaria salicornia and purified through DEAE (Diethylaminoethyl) - cellulose chromatography. The nanoparticles were prepared by the addition of sodium carbonate and poly-L- lysine. FTIR (Fourier-transform Infrared Spectroscopy) and 1H NMR (Nuclear Magnetic Resonance) analyses indicates that the isolated polysaccharide as sulphated galactan type. The zeta potential of the synthesized nanoparticle (GNPs) is -41 mV which denotes its stability. HRTEM (High-resolution Transmission Electron Microscope) analysis shows well dispersed spherical shaped nanoparticles with the size range between 20 and 50 nm. XRD (X-ray diffraction) analysis indicates the amorphous nature of the nanoparticle. Application of GNPs to tomato seeds showed an enhanced seedling vigor index. The nanoparticles (GNPs) exhibited antifungal activity against the most devastating phytopathogenic fungi of tomato viz., Alternaria solani, Fusarium oxysporum under in vitro condition. These results indicate that the GNPs prepared from biomass of marine seaweed can be used to control soil borne pathogenic fungi of tomato as an eco-friendly approach.
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Author Information
Department of Botany, School of Life Sciences, Bharathidasan University, Tiruchirappalli, India