Phytochemical contribution of Baccharis cinerea (Asteraceae) to the botanical origin of the greenish-brown propolis from northeastern Brazil

*Article not assigned to an issue yet

, , , , ,


Research Articles | Published:

E-ISSN: 2229-4473.
Website: www.vegetosindia.org
Pub Email: contact@vegetosindia.org
DOI: 10.1007/s42535-026-01726-3
First Page: 0
Last Page: 0
Views: 91

Keywords: Artepillin C, Antioxidant capacity, Compositeae, Botanical source, UFLC-DAD


Abstract


Propolis is a resin collected by bees, whose chemical composition varies with local vegetation. This study investigated Baccharis cinerea as a potential botanical source of greenish-brown propolis from Alagoas, Brazil. UFLC-DAD analyses demonstrated the shared presence of cinnamic acid derivatives between the propolis and the plant, including caffeic acid, ferulic acid, and artepillin C, detected between 7–26 min (λmax 315 nm). The propolis (C-PRO) presented high contents of total phenolics (66.62%) and flavonoids (10.44%), while the extracts from the flowering branches ranged from 15.83–55.48% and 1.31–10.42%, respectively. In the DPPH assay, the propolis exhibited an IC₅₀ of 15.03 µg/mL, and the plant extracts ranged from 4.34–44.11 µg/mL. The results suggest B. cinerea as one of the possible botanical origins of the greenish-brown propolis from the mountainous region of Alagoas, based on the sharing of phenolic markers, representing the first record of this association.

Artepillin C, Antioxidant capacity, Compositeae, Botanical source, UFLC-DAD


References


Abad MJ, Bermejo P, Sanchez Palomino S et al (1999) Antiviral activity of some South American medicinal plants. Phytother Res 13:142–146. https://doi.org/10.1002/(SICI)1099-1573(199903)13:2%3c142::AID-PTR392%3e3.0.CO;2-7


Agudelo I, Cogoi L, Filip R, Kuzmanich N, Wagner ML, Ricco RA (2018) Anatomy, histochemistry, and comparative analysis of hydroxycinnamic derivatives in healthy leaves and galls induced by Baccharopelma spp. (Hemiptera: Psyllidae) in Baccharis spicata (Lam) Baill (Asteraceae). Biochem Syst Ecol 77:22–30. https://doi.org/10.1016/j.bse.2018.01.001


Almeida-Junior S et al (2024) In vivo anti-inflammatory activity of baccharin from Brazilian green propolis. Fitoterapia 175:105975. https://doi.org/10.1016/j.fitote.2024.105975


Bankova V, Boudourova-Krasteva G, Sforcin JM et al (1999) Phytochemical evidence for the plant origin of Brazilian propolis from São Paulo State. Z Naturforsch C 54:401–405. https://doi.org/10.1515/znc-1999-5-616


Bankova V, Popova M, Trusheva B (2018) The phytochemistry of the honeybee. Phytochemistry 155:1–11. https://doi.org/10.1016/j.phytochem.2018.07.007


Bastos EMAF, Santana RA, Calaça-Costa AGF et al (2011) Interaction between Apis mellifera L. and Baccharis dracunculifolia DC., that favours green propolis production in Minas Gerais. Braz J Biol 71:727–734. https://doi.org/10.1590/S1519-69842011000400018


Batista MAC, Rodrigues EN, Moura JPM, Ceron CS et al (2024) Green propolis: a review of pharmaceutical patents with potential therapeutic applications. Comb Chem High Throughput Screen. https://doi.org/10.2174/0113862073298470240522093733


Beserra FP, Gushiken LFS, Hussni MF et al (2021) Artepillin C as an outstanding phenolic compound of Brazilian green propolis for disease treatment: a review on pharmacological aspects. Phytother Res 35:2274–2286. https://doi.org/10.1002/ptr.6875


Brand-Williams W, Cuvelier ME, Berset C (1995) Use of a free radical method to evaluate antioxidant activity. LWT Food Sci Technol 28:25–30. https://doi.org/10.1016/S0023-6438(95)80008-5


Brasil (2017) Agência Nacional de Vigilância Sanitária. Resolução RDC nº 166, de 24 de julho de 2017. Guia para validação de métodos analíticos e bioanalíticos. Anvisa, Brasília. http://anvisalegis.datalegis.net/. Acesso em 28 ago 2025


Calabria LM, Emerenciano VP, Scotti MT, Mabry TJ (2009) Secondary chemistry of Compositae. In: Funk VA, Susanna A, Stuessy TF, Bayer RJ (eds) Systematics, evolution, and biogeography of Compositae. International Association for Plant Taxonomy, Vienna, pp 73–87


Campos FR et al (2016) Baccharis (Asteraceae): chemical constituents and biological activities. Chem Biodivers 13:1–17. https://doi.org/10.1002/cbdv.201400363


Daugsch A, Moraes CS, Fort P et al (2008) Brazilian red propolis—chemical composition and botanical origin. Evid Based Complement Alternat Med 5:435–441. https://doi.org/10.1093/ecam/nem057


De Melo JG et al (2010) Antiproliferative activity, antioxidant capacity and tannin content in plants of semi-arid northeastern Brazil. Molecules 15(12):8534–8542. https://doi.org/10.3390/molecules15128534


De Mendonça ICG, Porto ICCM, do Nascimento TG et al (2015) Brazilian red propolis: phytochemical screening, antioxidant activity and effect against cancer cells. BMC Complement Altern Med 15:1–14. https://doi.org/10.1186/S12906-015-0888-9





Do Nascimento TG, Silva AS, Lessa Constant PB et al (2018) Phytochemical screening, antioxidant and antibacterial activities of some commercial extract of propolis. J Apic Res 57:246–254. https://doi.org/10.1080/00218839.2017.1412563


Guimarães NSS et al (2012) Baccharis dracunculifolia, the main source of green propolis, exhibits potent antioxidant activity and prevents oxidative mitochondrial damage. Food Chem Toxicol 50:1091–1097. https://doi.org/10.1016/j.fct.2011.11.014


Heiden G, Baumgratz JFA, Esteves RL (2012) Baccharis subgen. Molina (Asteraceae) no estado do Rio de Janeiro, Brasil. Rodriguésia 63:649–687. https://doi.org/10.1590/S2175-78602012000300013


Heiden G (2026) Baccharis in Flora e Funga do Brasil. Jardim Botânico do Rio de Janeiro. https://floradobrasil.jbrj.gov.br/FB5177. Acesso em 30 mar 2026





Silva BB, Rosalen PL, Cury JA et al (2008). Chemical composition and botanical origin of red propolis, a new type of Brazilian propolis. Evid Based Complement Alternat Med 5:313–316. https://doi.org/10.1093/ecam/nem059


Park YK, Alencar SM, Aguiar CL (2002a) Botanical origin and chemical composition of Brazilian propolis. J Agric Food Chem 50:2502–2506. https://doi.org/10.1021/jf011432b


Park YK et al (2002b) Própolis produzida no sul do Brasil, Argentina e Uruguai: evidências fitoquímicas de sua origem vegetal. Cienc Rural 32:997–1003. https://doi.org/10.1590/S0103-84782002000600013


Park YK, Paredes-Guzman JF, Aguiar CL et al (2004) Chemical constituents in Baccharis dracunculifolia as the main botanical origin of southeastern Brazilian propolis. J Agric Food Chem 52:1100–1103. https://doi.org/10.1021/jf021060m


Ribeiro VP, Mejia JAA, Rodrigues DM et al (2023) Brazilian brown propolis: an overview about its chemical composition, botanical sources, quality control, and pharmacological properties. Rev Bras Farmacogn 33:288–305. https://doi.org/10.1007/S43450-023-00374-X


Santos CT, Teotônio LEO, Nascimento APL et al (2020) Assessment and antimicrobial modulating activity of the extract of Baccharis cinerea DC. from Cariri Cearense. Biosci J 36:2247–2254. https://doi.org/10.14393/BJ-v36n6a2020-42278


Sartor T, Xavier VB, Falcão MA et al (2013) Seasonal changes in phenolic compounds and in the biological activities of Baccharis dentata. Ind Crops Prod 51:355–359. https://doi.org/10.1016/j.indcrop.2013.09.018


Shahinozzaman M, Basak B, Emran R et al (2020) Artepillin C: a comprehensive review of its chemistry, bioavailability, and pharmacological properties. Fitoterapia 147:104775. https://doi.org/10.1016/j.fitote.2020.104775


Shanahan M, Simone-Finstrom M, Tokarz P et al (2024) Thinking inside the box: restoring the propolis envelope facilitates honey bee social immunity. PLoS ONE 19:e0291744. https://doi.org/10.1371/journal.pone.0291744


Silva FG, Oliveira CBA, Pinto JEBP et al (2007) Seasonal variability in the essential oils of wild and cultivated Baccharis trimera. J Braz Chem Soc 18:990–997. https://doi.org/10.1590/S0103-50532007000500017


Silva EG Jr, Borges ALTF, Oliveira JVL et al (2026) Seasonal variation of a new Brazilian greenish-brown propolis type: chemical composition and antioxidant, antimicrobial, and antileishmanial activities. Molecules 31:1447. https://doi.org/10.3390/molecules31091447


Simone-Finstrom M, Borba RS, Wilson M et al (2017) Propolis counteracts some threats to honey bee health. InSects 8:46. https://doi.org/10.3390/insects8020046

 


Author Information


Campus of Engineering and Agricultural Sciences, Federal University of Alagoas – CECA/UFAL, Rio Largo, Brazil