Genetic diversity using RAPD markers, mineral composition and their correlation in selected local landraces of finger millet [Eleusine coracana (L.) Gaertn.]

Research Articles | Published:

E-ISSN: 2229-4473.
Website: www.vegetosindia.org
Pub Email: contact@vegetosindia.org
DOI: 10.1007/s42535-019-00001-y
First Page: 1
Last Page: 10
Views: 3239

Keywords: Diversity, Eleusine coracana , Mineral constituents, Population, RAPD


Abstract

Twelve local cultivars of finger millet [Eleusine coracana (L.) Gaertn.] were evaluated for their molecular diversity and mineral composition. Twelve selected RAPD primers generated 162 amplified fragments with an average of 13.5 bands per primer and 86.08% polymorphism. The average PIC, EMR, MI and RP values were 0.30, 5.79, 1.75 and 5.97 respectively, per primer. The Jaccard’s similarity coefficient values ranged from 0.44 to 0.67. The dendrogram grouped 12 finger millet cultivars into three distinctive groups based on their divergence analysis which indicates that significant genetic diversity was present among the cultivars. Among the major elements, average calcium content was found to be highest in all the cultivars followed by sodium, sulphur and nitrogen. The average content of manganese was followed by zinc and iron between the minor elements. The correlation studies between genetic diversity using Shannon diversity index and mineral content showed positive correlation among all the minerals studied excluding nitrogen. Genetic diversity combined with mineral datasets will be useful for selection of suitable cultivars for improvement of nutritional values in finger millet varieties through conventional and molecular breeding approaches.

Diversity, Eleusine coracana , Mineral constituents, Population, RAPD


References

  1. Ahmed MS, Gardezi SD (2017) Molecular characterization of locally adopted sugarcane (Saccharum officinarum L.) varieties using microsatellite markers. J Anim Plant Sci 27:164–174

  2. Babu BK, Senthil N, Gomez SM, Biji KR, Rajendraprasad NS, Kumar SS, Babu RC (2007) Assessment of genetic diversity among finger millet (Eleusine coracana (L.) Gaertn.) accessions using molecular markers. Genet Resour Crop Evol 54:399–404

  3. Bantawa P, Das A, Ghosh PB, Mondal TK (2011) Detection of natural diversity of Gaultheria fragrantissima landraces by RAPDs: an endangered woody oil bearing plant of Indo-China Himalayas. Ind J Biotechnol 10:294–300

  4. Chandrashekar A (2010) Finger Millet: Eleusine coracana. In: Taylor SL (ed) Advances in food and nutrition research. Academic Press, Burlington, pp 215–262

  5. Datta K, Rai M, Parkhi V, Oliva N, Tan J, Datta SK (2006) Improved ‘golden’ indica rice and post-transgeneration enhancement of metabolic target products of carotenoids (b-carotene) in transgenic elite cultivars (IR64 and BR29). Curr Sci 91:935–939

  6. Devi PB, Vijayabharathi R, Sathyabama S, Malleshi NG, Priyadarisini VB (2014) Health benefits of finger millet (Eleusine coracana L.) polyphenols and dietary fiber: a review. J Food Sci Technol 51:1021–1040

  7. Doyle JJ, Doyle JL (1990) Isolation of plant DNA from fresh tissue. Focus 12:13–15

  8. Fakrudin B, Shashidhar HE, Kulkarni RS, Hittalmani S (2004) Genetic diversity assessment of finger millet, Eleusine coracana (Gaertn.), germplasm through RAPD analysis. PGR Newslett 138:50–54

  9. Gernand AD, Schulze KJ, Stewart CP, West KP Jr, Christian P (2016) Micronutrient deficiencies in pregnancy worldwide: health effects and prevention. Nat Rev Endocrinol 12:274–289

  10. Gonzàlez-Miret ML, Terrab A, Hernanz D, Fernàndez-Recamales MA, Heredia FJ (2005) Multivariate correlation between color and mineral composition of honeys and by their botanical origin. J Agric Food Chem 53:2574–2580

  11. Graham R, Senadhira D, Beebe S, Iglesias C, Monasteriod I (1999) Breeding for micronutrient density in edible portions of staple food crops: conventional approaches. Field Crops Res 60:57–80

  12. Grant CA, Clarke JM, Duguid S, Chaney RL (2008) Selection and breeding of plant cultivars to minimize cadmium accumulation. Sci Total Environ 390:301–310

  13. Guo X, Elston RC (1999) Linkage information content of polymorphic genetic markers. Hum Hered 49:112–118

  14. Gupta N, Gupta AK, Singh NK, Kumar A (2011) Differential expression of PBF Dof transcription factor in different tissues of three finger millet genotypes differing in seed protein content and color. Plant Mol Biol Rep 29:69–76

  15. Gupta SM, Arora S, Mirza N, Pande A, Lata C, Puranik S, Kumar J, Kumar A (2017) Finger Millet: a “certain” crop for an “uncertain” future and a solution to food insecurity and hidden hunger under stressful environments. Front Plant Sci 8:643

  16. Han T, Hu Y, Zhou SY, Li HL, Zhang QY, Zhang H, Huang BK, Rahman K, Zheng HC, Qin LP (2008) Correlation between the genetic diversity and variation of total phenolic acids contents in Fructus xanthii from different populations in China. Biomed Chromatogr 22:478–486

  17. Kumar A, Mishra P, Singh SC, Sundaresan V (2014a) Efficiency of ISSR and RAPD markers in genetic divergence analysis and conservation management of Justicia adhatoda L., a medicinal plant. Plant Syst Evol 300:1409–1420

  18. Kumar A, Mirza N, Charan T, Sharma N, Gaur VS (2014b) Isolation, characterization and immunolocalization of a seed dominant CaM from finger millet (Eleusine coracana L. Gartn.) for studying its functional role in differential accumulation of calcium in developing grains. Appl Biochem Biotechnol 172:2955–2973

  19. Kumar A, Metwal M, Kaur S, Gupta AK, Puranik S, Singh S, Singh M, Gupta S, Babu BK, Sood S, Yadav R (2016) Nutraceutical value of finger millet [Eleusine coracana (L.) Gaertn.], and their improvement using omics approaches. Front Plant Sci 7:934

  20. Latha AM, Rao KV, Reddy VD (2005) Production of transgenic plants resistant to leaf blast disease in finger millet (Eleusine coracana (L.) Gaertn.). Plant Sci 169:657–667

  21. Lokhande VH, Nikam TD, Patade VY, Suprasanna P (2009) Morphological and molecular diversity analysis in Indian clones of Sesuvium portulacastrum L. Genet Resour Crop Evol 56:705–717

  22. Naikawadi VB, Ahire ML, Lokhande VH, Ghorpade RP, Nikam TD (2016) Morphological, biochemical and molecular characterization of Evolvulus alsinoides Linn.: a memory enhancing herb. Ind J Biotechnol 15:48–56

  23. Najaphy A, Parchin RA, Farshadfar E (2011) Evaluation of genetic diversity in wheat cultivars and breeding lines using inter simple sequence repeat markers. Biotechnol Biotechnol Equip 25:2634–2638

  24. Nedumaran S, Bantilan MCS, Gupta SK, Irshad A, Davis JS (2014) Potential welfare benefit of millets improvement research at ICRISAT: multi country-economic surplus model approach, socioeconomics discussion paper series number 15. ICRISAT, Hyderabad

  25. O’Kennedy MM, Grootboom A, Shewry PR (2006) Harnessing sorghum and millet biotechnology for food and health. J Cereal Sci 44:224–235

  26. Ortiz-Monasterio JI, Palacios-Rojas N, Meng E, Pixley K, Trethowan R, Peña RJ (2007) Enhancing the mineral and vitamin content of wheat and maize through plant breeding. J Cereal Sci 46:293–307

  27. Panwar P, Saini RK, Sharma N, Yadav D, Kumar A (2010) Efficiency of RAPD, SSR and cytochrome P450 gene based markers in accessing genetic variability amongst finger millet (Eleusine coracana) accessions. Mol Biol Rep 37:4075–4082

  28. Ramakrishnan M, Ceasar SA, Duraipandiyan V, Al-Dhabi NA, Ignacimuthu S (2015) Using molecular markers to assess the genetic diversity and population structure of finger millet (Eleusine coracana (L.) Gaertn.) from various geographical regions. Genet Resour Crop Evol 63:361–376

  29. Ramakrishnan M, Ceasar SA, Duraipandiyan V, Al-Dhabi NA, Ignacimuthu S (2016) Assessment of genetic diversity, population structure and relationships in Indian and non-Indian genotypes of finger millet (Eleusine coracana (L.) Gaertn.) using genomic SSR markers. SpringerPlus 5:120

  30. Renuka N, Mathure SV, Zanan RL, Thengane RJ, Nadaf AB (2016) Determination of some minerals and b-carotene contents in aromatic indica rice (Oryza sativa L.) germplasm. Food Chem 191:2–6

  31. Rohlf FJ (1992) NTSYS-pc. Numerical Taxonomy and multivariate analysis system: version 2.02e. Applied Biostatistics, New York

  32. Shashi BK, Sunanda S, Shailaja H, Shankar AG, Nagarathna TK (2007) Micronutrient composition, antinutritional factors and bioaccessibility of iron in different finger millet (Eleusine coracana) genotypes. Karnataka J Agric Sci 20:583–585

  33. Singh P, Singh SP, Tiwari AK, Sharma BL (2017) Genetic diversity of sugarcane hybrid cultivars by RAPD markers. 3 Biotech 7:222

  34. Stein AJ (2010) Global impacts of human malnutrition. Plant Soil 335:133–154

  35. Talebi R, Haghnazari A, Tabatabaei I (2010) Assessment of genetic variation within international collection of Brassica rapa genotypes using inter simple sequence repeat DNA markers. Biharean Biologist 4:145–151

  36. Tatikonda L, Wani SP, Kannan S, Beerelli N, Sreedevi TK, Hoisington DA, Devi P, Varshney RK (2009) AFLP-based molecular characterization of an elite germplasm collection of Jatropha curcas L., a biofuel plant. Plant Sci 176:505–513

  37. Thudi M, Manthena R, Wani SP, Tatikonda L, Hoisington DA, Varshney RK (2010) Analysis of genetic diversity in Pongamia [Pongamia pinnata (L) Pierrre] using AFLP markers. J Plant Biochem Biotechnol 19:209–216

  38. Tripathi B, Platel K (2010) Finger millet (Eleucine coracana) flour as a vehicle for fortification with zinc. J Trace Elem Med Biol 24:46–51

  39. Vadivoo AS, Joseph R, Ganesan NM (1998) Genetic variability and diversity for protein and calcium contents in finger millet (Eleusine coracana (L.) Gaertn) in relation to grain color. Plant Foods Hum Nutr 52:353–364

  40. Vinoth A, Ravindhran R (2017) Biofortification in Millets: a sustainable approach for nutritional security. Front Plant Sci 8:29

  41. Williams JGK, Kubelik AR, Livak KJ, Reafalski JA, Tingey SV (1990) DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acid Res 18:6531–6535

  42. Yadav OP, Rai KN (2013) Genetic improvement of pearl millet in India. Agric Res 2:275–292


    43.  


Author Information

Department of Botany, Savitribai Phule Pune University, Pune, India