Post-harvest management for preserving bergenin content in Bergenia ciliata rhizomes and dried extract

*Article not assigned to an issue yet

, , , ,


Research Articles | Published:

E-ISSN: 2229-4473.
Website: www.vegetosindia.org
Pub Email: contact@vegetosindia.org
DOI: 10.1007/s42535-026-01807-3
First Page: 0
Last Page: 0
Views: 89

Keywords: n Bergenia ciliatan , Bergenin, Drying, Rhizome storage, Room temperature, Refrigerated conditions


Abstract


The present study was conducted to investigate the dynamics of bergenin content in Bergenia ciliata rhizomes when subjected to different drying and storage conditions during 2023–24. Freshly collected rhizomes were subjected to various methods of drying viz., shade drying, sun drying, solar drying and oven drying at 35 °C, 40 °C, 60 °C and 70 °C. Among the different drying methods, shade drying preserved the highest bergenin content (5.058 mg/g of crude extract), showing a significant advantage over the other drying methods. During storage, the effect of packaging material, storage conditions and duration on bergenin content was evaluated. The shade dried rhizome samples were stored at room temperature (25 °C), refrigerator (4– 6 °C) and deep freezer (− 20 °C) conditions for 2, 4 and 6 months. Bergenin content in dried rhizomes and dried rhizome extract declined as storage duration increased irrespective of the different storage conditions. The bergenin content in rhizomes decreased from 8.50 ± 0.08 to 7.57 ± 0.08 mg/g of crude extract after six months of storage at 4–6 °C. Whereas, bergenin content in dried rhizome extract decreased from 34.26 ± 0.03 to 25.03 ± 0.02 mg/g of crude extract after six month of storage at − 20 °C. Refrigeration (4–6 °C) enhanced bergenin retention compared to room temperature, although all storage conditions experienced notable losses. Among the drying methods, shade drying proved most effective in preserving bergenin content. This is the first report of evaluation of different drying and storage conditions on stability of bergenin in Bergenia ciliata providing practical guidelines for long-term storage and industrial applications.

n                     Bergenia ciliatan                  , Bergenin, Drying, Rhizome storage, Room temperature, Refrigerated conditions


References


Abdullah S, Shaari AR, Azimi A (2012) Effect of drying methods on metabolites composition of misaikucing (Orthosiphon stamineus) leaves. APCBEE Proc 2:178–182


Ahmad M, Butt MA, Zhang G, Sultana S, Tariq A, Zafar M (2018) Bergenia ciliata: A comprehensive review of its traditional uses, phytochemistry, pharmacology and safety. Biomed Pharmacother 97:708–721


Arok R, Végh K, Alberti Á, Kéry Á (2012) Phytochemical comparison and analysis of Bergenia crassifolia L.(Fritsch.) and Bergenia cordifolia Sternb. Eur Chem Bull 1(12):31–34


Babarabie M, Mohammadi M, Ghorbanzadeh A, Afsharipour S, Salari F (2025) Effect of drying conditions on the preservation of selected bioactive compounds in Moringa oleifera aqueous extract: acetic acid, butyric acid, γ-aminobutyric acid, salicin, and glycine. BMC Plant Biol 25:1432


Balekundri A, Mannur V (2020) Quality control of the traditional herbs and herbal products: a review. Future J Pharm Sci 6:1–9


Bharate SB, Kumar V, Bharate SS, Singh B, Singh G, Singh A, Gupta M, Singh D, Kumar A, Singh S, Vishwakarma RA (2019) Discovery and preclinical development of IIIM-160, a Bergenia ciliata-based anti-inflammatory and anti-arthritic botanical drug candidate. J Integr Med 17(3):192–204


Bhat MA, Raina R, Verma PK, Sood S, Bhat ZF (2024) Ameliorative and protective effect of Bergenia ciliata (Haw.) Sternb extract against acetaminophen-induced hepato-renal damage in rats: B. ciliata and hepato-renal damage. Phytomed plus 4(1):100522


Chaudhuri KM, Debnath MK, Dutta P (2024) Studies on viability and efficacy of various invigoration techniques to improve germination parameters of Bergenia ciliata (Haw.) Sternb. J Appl Res Med Aromat Plants 41:100556


Chauhan V, Rawat P, Chauhan N (2021) Review on compilation of ethnopharmacological properties of Bergenia ciliata: the medicinal herb of Himalayas. Plant Sci Arch. 21(2):9725210


Chowdhary S, Kumar H, Verma DL (2009) Chemical examination of Bergenia stracheyi (Hk.) for antioxidative flavonoids. Nat sci. 7(4).


Del-Toro Sánchez CL, Gutiérrez-Lomelí M, Lugo-Cervantes E, Zurita F, Robles-García MA, Ruiz-Cruz S, Aguilar JA, Rio Ja M, Guerrero-Medina PJ (2015) Storage effect on phenols and on the antioxidant activity of extracts from Anemopsis californica and inhibition of elastase enzyme. J Chem 7(1):1–8


Dharmender R, Madhavi T, Reena A, Sheetal A (2010) Simultaneous Quantification of Bergenin, (+)-Catechin, Gallicin and Gallic acid; and quantification of β-Sitosterol using HPTLC from Bergenia ciliata (Haw.) Sternb. Pharm Anal Acta 1:104


Dulta K, Koşarsoy Ağçeli G, Chauhan P, Jasrotia R, Chauhan PK (2021) A novel approach of synthesis zinc oxide nanoparticles by Bergenia ciliata rhizome extract: antibacterial and anticancer potential. J Inorg Organomet Polym Mater 31:180–190


Faiz S, Waseem D, Haq IU, Taqi MM, Mohsin SA, Irshad N, Phull AR, Fatima H (2023) Comparative appraisal of in vitro biological profile and in vivo wound healing attributes of bergenin and Bergenia ciliata (Haw.) Sternb. J Ethnopharmacol 304:115–993


Ghabaei T, Nazirzadeh S, Nourafcan H (2019) Effect of different drying methods on quantity and quality of active substances of Purple coneflower (Echinacea purpureae L.). J Herbal Drugs: Int J Herb Med 9:115–120


Ghorbani A, Eghlima G, Farzaneh M, Ayyub R (2025) Effect of drying methods on mucilage, anthocyanin content, and antioxidant activity of black hollyhock (Alcea rosea var. nigra). BMC Plant Biol 25:478


Gyawali R, Kim KS (2012) Bioactive volatile compounds of three medicinal plants from Nepal. J Eng Sci Technol 8(1):51–62


Harbourne N, Marete E, Jacquier JC, O’Riordan D (2009) Effect of drying methods on the phenolic constituents of meadowsweet (Filipendula ulmaria) and willow (Salix alba). LWT-Food Sci and Technol 42(9):1468–1473


Ibrahim MN, Chong GH (2008) Stability of andrographolide in Andrographis paniculata under selected storage conditions. Int J Eng Res Technol 5(1):69–73


Kanth M, Hussain A, Shrivastva PK, Sharma M, Tripath J, Khan MA (2019) Extraction, qualitative and quantitative determination of secondary metabolites of Bergenia ciliata (Haw) Sternb rhizome. J Drug Deliv Ther 9(3):407–411


Koul B, Kumar A, Yadav D, Jin JO (2020) Bergenia genus: Traditional uses, phytochemistry and pharmacology. Molecules 25(23):5555


Kour H, Raina R, Verma PK, Khan AM, Bhat MA, Nashiruddullah N (2021) Evaluation of the wound healing activity of ethanolic extract of Bergenia ciliata (Haw.) Sternb. rhizome with excision wound model in Wistar rats. J Ethnopharmacol 281:114–527


Koyu H, Haznedaroglu MZ (2015) Investigation of impact of storage conditions on Hypericum perforatum L. dried total extract. J Food Drug Anal 23(3):545–551


Kushwaha N, Singh A (2024) Bergenia ciliata - Phytochemistry and Pharmacology: A Review. Biomed Mater. 1–14.


Latief U, Tung GK, Singh H, Per TS, Jain SK (2022) Bergenia ciliata as a future candidate for liver diseases: a concise review. J Basic Appl Zool 83(1):17


Moktan N, Banerjee A (2024) Polypharmacological Constituents and Potential Activities of Bergenia ciliata: A Concise Review: Natural Product Chemistry. Innov Chem Mater Sustain 1(1):58–65


Mukherjee K, Sengupta S, Singh AK, Tonk R, Azizov S, Rajwade RP, Kumar D (2026) Therapeutic potential of Bergenia ciliata against lung cancer: an integrative molecular docking, ADMET and molecular dynamics approach. In Silico Pharmacology 14:38


Ndhlala AR, Ngobeni GT, Mulaudzi R, Lebelo SL (2025) Different temperature storage conditions and packaging types affects colour parameters, amino acid composition, microbial contamination, and key bioactive molecules of Moringa oleifera Lam. powder. Molecules 30(20):4048


Padmapriya S, Kumanan K, Rajamani K (2009) Optimization of post harvest techniques for Tinospora cordifolia. J Plant Sci 2(3):128–310


Qin X, Zhou D, Zhang ZR, Huang Y (2007) Determination of bergenin in rat plasma by high-performance liquid chromatography. Pharmazie 62(5):323–326


Rehman S, Iqbal Z, Qureshi R, AlOmar TS, Almasoud N, Younas M, Rauf A, Irfan M (2024) Ethno-Dentistry of Medicinal Plants Used in North Waziristan. Pakistan Int Dent J 74(2):310–320


Sapkota BK, Khadayat K, Aryal B, Bashyal J, Jaisi S, Parajuli N (2022) LC-HRMS-based profiling: antibacterial and lipase inhibitory activities of some medicinal plants for the remedy of obesity. Sci Pharm 90(3):55


Skrajda-Brdak M, Dąbrowski G, Konopka I (2020) Edible flowers as a source of valuable phytonutrients and their pro-health effects - A review. Trends Food Sci Technol 103:179–19


Tanko H, Carrier DJ, Duan L, Clausen E (2005) Pre-and post-harvest processing of medicinal plants. Plant Genet Res 3(2):304–313


Thani PR, Sharma YP (2016) Standardization of storage conditions and duration on Picroside-I and Picroside-II in raw material of drug kutki (Picrorhiza kurroa Royle ex Benth.). Nepal Journal of Science and Technology 17(1):23–26


Vidyashankar S, Maheshkumar P, Patki PS (2010) Cystone-An ayurvedic polyherbal formulation inhibits adherence of uropathogenic E. coli and modulates H2O2-induced toxicity in NRK-52E cells. J Exp Pharmacol 19–27.


Wills RBH, Shohet D (2009) Changes in valerenic acids content of valerian root (Valeriana officinalis L. sl) during long-term storage. Food Chem. 115(1), 250–253.


Zhou D, Qin X, Zhang ZR, Huang Y (2008) Physicochemical properties of bergenin. Pharmazie 63(5):366–371


Zothantluanga JH, Chagaleti BK, Roy D, Abdalla M, El-Arabey AA, Alahmady NF, Jha NK (2026) Dual inhibition of AChE and GSK-3β by flavonoids of Bergenia ciliata: molecular dynamics insights into anti-Alzheimer’s activity. Comput Biol Chem 122:108908


de Mendiburu F (2019) Agricolae: Statistical Procedures for Agricultural Research. R package version 1.3-1.


Peter KV (2006) Handbook of Herbs and Spices (Volume 3). Woodhead Publishing Limited, Cambridge, UK

 


Author Information


Department of Forest Products, Dr. YS Parmar University of Horticulture & Forestry, Nauni- Solan, India