Vegetos
(An International Journal of Plant Research & Biotechnology)
(eISSN: 2229-4473)
Aluminium alleviates fluoride toxicity in Cyamopsis tetragonoloba by enhancing growth performance and reducing fluoride uptake
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Research Articles | Published: 20 May, 2026
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Keywords: Fluoride, Aluminium, Cluster bean, Accumulation
Abstract
Fluoride pollution in agricultural soils has become a major global concern, reducing crop output and quality. In contaminated areas, fluoride stress has an impact on the productivity of cluster bean (Cyamopsis tetragonoloba L. Taub.), a legume of significant economic value. Fluoride mobility in soil is governed by its distribution between total and soluble forms, which is strongly influenced by soil pH and mineral interactions affecting its bioavailability. This study examined how aluminium can reduce fluoride toxicity in cluster bean varieties. Both separately and in combination, soil was treated with varying quantities of fluoride (0, 200, and 400 mg kg−1) and aluminium (0, 200, and 300 mg kg−1). The highest binding affinity of any metal ion for F⁻ is shown by Al3+, which forms Al-F complexes that decrease fluoride toxicity and bioavailability, especially in acidic environments. The overall plant growth under the fluoride stress was lowered in all five varieties than in control. The greatest decline in growth metrics was noted in HG-2-20 followed by RGC-1033, HG-563, RGC-1066, and RGC-936. Aluminium treatment effectively reduces the negative effects of fluoride by restricting fluoride uptake. These findings show that controlled aluminium supplementation with fluoride could be a useful strategy to improve plant resistance to fluoride toxicity.
References
Ahmad MN, Zia A, Van den Berg L, Ahmad Y, Mahmood R, Dawar KM, Ashmore M (2022) Effects of soil fluoride pollution on wheat growth and biomass production, leaf injury index, powdery mildew infestation and trace metal uptake. Environ Pollut 298(3):118820. https://doi.org/10.1016/j.envpol.2022.118820
Asztemborska M, Steborowski R, Kowalska J, Bystrzejewska-Piotrowska G (2015) Accumulation of aluminium by plants exposed to nano-and microsized particles of Al2O3. Int J Environ Res 9(1):109–116
Banerjee A, Roychoudhury A (2019) Differential regulation of defence pathways in aromatic and non-aromatic indica rice cultivars towards fluoride toxicity. Plant Cell Rep 38(10):1217–1233. https://doi.org/10.1007/s00299-019-02438-6
Bell LE, Moir JL, Black AD (2024) The effects of soil acidity and aluminium on the root systems and shoot growth of Lotus pedunculatus and Lupinus polyphyllus. Plants Basel 13(16):2268. https://doi.org/10.3390/plants13162268
Cameron RS, Ritchie GSP, Robson AD (1986) Relative toxicities of inorganic aluminum complexes to barley. Soil Sci Soc Am J 50(5):1231–1236. https://doi.org/10.2136/sssaj1986.03615995005000050029x
Choudhary S, Rani M, Singh RK, Devika S, Patra A, Prasad SK (2019) Impact of fluoride on agriculture: a review on it’s sources, toxicity in plants and mitigation strategies. Int J Chem Stud 7(2):1675–1680
Dawood MFA, Tahjib-Ul-Arif M, Sohag AAM, Abdel Latef AAH (2022) Fluoride mitigates aluminum-toxicity in barley: morpho-physiological responses and biochemical mechanisms. BMC Plant Biol 22(1):287. https://doi.org/10.1186/s12870-022-03610-z
Deng Z, Xu A, Wei X, Zhang X, Pan B (2024) Formation of aluminum-fluoride complexes compromises defluoridation efficiency of aluminum-based coagulation. Environ Sci Technol 58(33):14918–14928. https://doi.org/10.1021/acs.est.4c04722
Essebbahi I, Ouazzani C, Moustaghfir A, Er-ramly A, El Baroudi Y, El Baroudi H, Dami A, Balouch L (2024) Assessment of fluoride accumulation in various vegetable and leafy greens consumed in Morocco. J Saudi Soc Agric Sci 23(5):340–344. https://doi.org/10.1016/j.jssas.2024.03.001
Foster HD (2004) Capes, bays and the double helix: why geography has more to offer in the prevention of chronic degenerative diseases than genetics. J Orthomol Med 19(1):39–47
Frankowski M, Zioła-Frankowska A, Siepak J (2010) Speciation of aluminium fluoride complexes and Al3+ in soils from the vicinity of an aluminium smelter plant by hyphenated high performance ion chromatography flame Atomic Absorption Spectrometry technique. Microchem J 95(2):366–372. https://doi.org/10.1016/j.microc.2010.02.019
Geretharan T, Jeyakumar P, Bretherton M, Anderson CW (2018) Defining a standard method to measure the total and bioavailable concentration of fluorine in New Zealand soils. Microchem J 142:94–101. https://doi.org/10.1016/j.microc.2018.06.018
Guo C, Shabala S, Chen ZH, Zhou M, Zhao C (2024) Aluminium tolerance and stomata operation: towards optimising crop performance in acid soil. Plant Physiol Biochem 210:108626. https://doi.org/10.1016/j.plaphy.2024.108626
Gurmu G, Beyene S, Selassie YG (2025) Aluminum alters soil dynamics and wheat micronutrient uptake under limed and unlimed acidic soil conditions. Discov Soil 2(1):75. https://doi.org/10.1007/s44378-025-00084-x
Imran QM, Falak N, Hussain A, Mun BG, Yun BW (2021) Abiotic stress in plants, stress perception to molecular response and role of biotechnological tools in stress resistance. Agronomy 11(8):1579. https://doi.org/10.3390/agronomy11081579
Ji CH, Lee SH, Bae GS, Kim HW (2024) Balancing environmental safety and economic feasibility: a review of soil fluorine management strategies in South Korea. Sustainability 16(19):8391. https://doi.org/10.3390/su16198391
Kamiński J, Stachelska-Wierzchowska A, Michalczyk DJ, Klimkowicz-Pawlas A, Olkowska E, Wolska L, Piotrowicz-Cieślak AI (2024) Changes in metabolism and content of chlorophyll in common duckweed (Lemna minor L.) caused by environmental contamination with fluorides. Molecules 29(10):2336. https://doi.org/10.3390/molecules29102336
Katiyar P, Pandey N, Varghese B, Sahu KK (2025) Biopriming of Pseudomonas aeruginosa abates fluoride toxicity in Oryza sativa L. by restricting fluoride accumulation, enhancing antioxidative system, and boosting activities of rhizospheric enzymes. Plants 14(8):1223. https://doi.org/10.3390/plants14081223
Kumar R, Sinha R, Sharma PK, Ivy N, Kumar P, Kant N, Jha A, Jha PK, Gupta PK, Sharma P, Singh RK, Singh RP, Ghosh A, Vara Prasad PV (2021) Bioaccumulation of fluoride in plants and its microbially assisted remediation: a review of biological processes and technological performance. Processes 9(12):2154. https://doi.org/10.3390/pr9122154
Kumari N, Yadav M, Sharma V (2018) Differential response of Brassica juncea cultivars to Al; consequences for chlorophyll a fluorescence, antioxidants and psb A gene. J Plant Interact 13(1):496–505. https://doi.org/10.1080/17429145.2018.1526980
Little I (1964) The determination of exchangeable aluminium in soils. Aust J Soil Res 2(1):76–82. https://doi.org/10.1071/SR9640076
Manoharan V, Loganathan P, Tillman RW, Parfitt RL (2007) Interactive effects of soil acidity and fluoride on soil solution aluminium chemistry and barley (Hordeum vulgare L.) root growth. Environ Pollut 145(3):778–786. https://doi.org/10.1016/j.envpol.2006.05.015
Matus P, Kubova J, Bujdos M (2006) Free aluminium extraction from various reference materials and acid soils with relation to plant availability. Talanta 70(5):996–1005. https://doi.org/10.1016/j.talanta.2006.05.045
McQuaker NR, Gurney M (1977) Determination of total fluoride in soil and vegetation using an alkali fusion-selective ion electrode technique. Anal Chem 49(1):53–56. https://doi.org/10.1021/ac50009a022
Pan J, Li D, Zhu J, Shu Z, Ye X, Xing A, Wen B, Ma Y, Zhu X, Fang W, Wang Y (2020) Aluminum relieves fluoride stress through stimulation of organic acid production in Camellia sinensis. Physiol Mol Biol Plants 26(6):1127–1137. https://doi.org/10.1007/s12298-020-00813-2
Pareek S, Kumari K, Awan T, Sharma A, Shrivastava D (2022) Guar (Cyamopsis tetragonoloba [L.] Taub.) response to organic and inorganic nitrogen sources during early seedling growth. J Plant Nutr 45(14):2135–2145. https://doi.org/10.1080/01904167.2022.2046072
Sharma K, Verma K, Meena AK (2023) Biochemical and molecular behaviour of clusterbean and mungbean against fluoride stress. J Pharm Innov 12(3):1973–1976
Sharma V, Yadav M, Kumari N (2018) Aluminium fluoride induced changes in chlorophyll a fluorescence, antioxidants and psb a gene expression of Brassica juncea cultivars. J Plant Interact 13(1):472–482. https://doi.org/10.1080/17429145.2018.1522001
Shi H, Lv R, Liu Y, Xiao D, Wang Z, Yuan X, Liu L, Yu C (2023) Spatial variability characteristics and influencing factors of soil fluoride in the Western Nansihu Lake Basin. Water 15(21):3855. https://doi.org/10.3390/w15213855
Singh G, Sinam G, Pandey MK, Kumari B, Kulsoom M (2019) Soil pollution by fluoride in India: Distribution, chemistry and analytical methods. In: Environmental Concerns and Sustainable Development, Volume 2: Biodiversity, Soil and Waste Management. Springer, Singapore, pp 307–324. https://doi.org/10.1007/978-981-13-6358-0_12
Takamoto A, Takahashi T, Togami K (2023) Estimation models from soil pH with a solid-to-liquid ratio of 1: 2.5 to pH measured by other methods using soils in Japan. Soil Sci Plant Nutr 69(3):190–198. https://doi.org/10.1080/00380768.2023.2190749
Umrao V, Yadav S, Semwal P, Misra S, Mishra SK, Chauhan PS, Shirke PA (2024) Endophytic bacilli from Cyamopsis tetragonoloba (L.) Taub. induces plant growth and drought tolerance. Int J Microbiol 27(5):1541–1556. https://doi.org/10.1007/s10123-024-00499-6
Verma KK, Verma P, Singh M, Verma CL (2022) Influence of fluoride phytotoxicity in germinating seedlings of Pisum sativum: modeling of morpho-physiological traits. Vegetos 35(3):736–746. https://doi.org/10.1007/s42535-021-00333-8
Vishnyakova MA, Frolova N, Frolov A (2023) Drought stress response in Guar (Cyamopsis tetragonoloba (L.) Taub): physiological and molecular genetic aspects. Plants 12(23):3955. https://doi.org/10.3390/plants12233955
Wambu EW, Kurui AJ (2018) Fluoride adsorption onto soil adsorbents: the role of pH and other solution parameters. In: Soil pH for nutrient availability and crop performance, IntechOpen, pp 1508–1520. https://doi.org/10.5772/intechopen.74652
Yadav M, Kumari N, Sharma V (2018) Phytoremediation efficiency of Brassica juncea cultivars at vegetative and reproductive growth stages under individual and combined treatment of fluoride and aluminium. Int J Phytoremediation 20(9):922–929. https://doi.org/10.1080/15226514.2018.1448361
Yang Y, Liu Y, Huang CF, de Silva J, Zhao FJ (2016) Aluminium alleviates fluoride toxicity in tea (Camellia sinensis). Plant Soil 402:1–2. https://doi.org/10.1007/s11104-015-2787-8
Zhang H, Jiang Z, Qin R, Zhang H, Zou J, Jiang W, Liu D (2014) Accumulation and cellular toxicity of aluminum in seedling of Pinus massoniana. BMC Plant Biol 14(1):264. https://doi.org/10.1186/s12870-014-0264-9
Zouari M, Elloumi N, Bellassoued K, Ben Ahmed C, Krayem M, Delmail D, Elfeki A, Ben Rouina B, Ben Abdallah F, Labrousse P (2017) Enzymatic antioxidant responses and mineral status in roots and leaves of olive plants subjected to fluoride stress. S Afr J Bot 111:44–49. https://doi.org/10.1016/j.sajb.2017.03.039
Author Information
Department of Bioscience and Biotechnology, Banasthali Vidyapith, Tonk, India