Vegetos
(An International Journal of Plant Research & Biotechnology)
To study the effect of salt tolerant plant growth promoting bacterial consortium on growth of maize (Zea mays L). under the salt stress condition
*Article not assigned to an issue yet
Kaneriya Jinesh, Pattani Vivek, Joshi Krishna, Sanghvi Gaurav
Research Articles | Published: 11 April, 2025
First Page: 0
Last Page: 0
Views: 406
Keywords: Biocontrol, Consortium, Maize, Phosphate solubilization, Saline, Siderophore
Abstract
Maize (Zea mays L.), a staple food consumed worldwide, has extensive applications across various industries, including paper production, ethanol manufacturing, and the food sector. In our study, We conducted a series of experiments to assess the growth-enhancing abilities of selected bacteria, focusing on their capacity for siderophore production, phosphate and zinc solubilization, emission of hydrogen cyanide and their antifungal activity against Fusarium Spp. Out of 35 plant growth-promoting bacterial isolates 14 indigenous PGPBs were identified for in detail analysis. Our methodological approach comprised four levels of rigorous screening, employing a combination of PGPB consortia. Each screening phase applied, to restrict from 509 potential combinations to 7 that exhibited significant enhancements for all biochemical parameters under salinity conditions of 100, 200, and 300 mM. Furthermore, physiological parameters carotenoids, Anthocyanin and proline content of the plants with bacterial consortium inoculation, concerning untreated control in the presence of salt stress. The use of a bacterial consortium has the potential to stimulate plant growth and could be considered a bioinoculant for improving maize crops.
References
Bric JM, Bostock RM, Silverstone SE (1991) Rapid in situ assay for indoleacetic acid production by bacteria immobilized on a nitrocellulose membrane. Appl Environ Microbiol 57(2):535–538. https://doi.org/10.1128/aem.57.2.535-538.1991
Carlos Rodriguez L (2022) Application of discrete mathematics for programming discrete mathematics calculations. In: Kamal S, Bruno C, Arshad A (eds). Qualitative and computational aspects of dynamical systems (pp. Ch. 4). IntechOpen, Rijeka
Cochard B, Giroud B, Crovadore J, Chablais R, Arminjon L, Lefort F (2022) Endophytic PGPR from tomato roots: isolation, in vitro characterization and in vivo evaluation of treated tomatoes (Solanum lycopersicum L). Microorganisms 10(4):765. https://doi.org/10.3390/microorganisms10040765
Egamberdieva D, Kucharova Z, Davranov K, Berg G, Makarova N, Azarova T et al (2011) Bacteria able to control foot and root rot and to promote growth of cucumber in salinated soils. Biol Fertil Soils 47:197–205.https://doi.org/10.1007/s00374-010-0523-3
Gong Z, Xiong L, Shi H, Yang S, Herrera-Estrella LR, Xu G et al (2020) Plant abiotic stress response and nutrient use efficiency. Sci China Life Sci 63:635–674. https://doi.org/10.1007/s11427-020-1683-x
Gupta A, Gopal M (2008) Siderophore production by plant growth promoting rhizobacteria. Indian J Agric Res 42(2):153–156
He W, Wang Y, Luo H, Li D, Liu C, Song J, Zhang Z, Liu C, Niu L (2020) Effect of NaCl stress and supplemental CaCl2 on carotenoid accumulation in germinated yellow maize kernels. Food Chem 309:125779. https://doi.org/10.1016/j.foodchem.2019.125779
Hyder S, Gondal AS, Rizvi ZF, Atiq R, Haider MIS, Fatima N, Inam-ul-Haq M (2021) Biological control of Chili damping-off disease, caused by pythium myriotylum. Front Microbiol 12:587431. https://doi.org/10.3389/fmicb.2021.587431
Jangra A, Kumar K, Maikhuri S, Bhandari MS, Pandey S, Singh H, Barthwal S (2024) Unveiling stress-adapted endophytic bacteria: characterizing plant growth-promoting traits and assessing cross-inoculation effects on Populus deltoides under abiotic stress. Plant Physiol Biochem 210:108610. https://doi.org/10.1016/j.plaphy.2024.108610
Jeong J-J, Sajidah S, Oh JY, Sang MK, Kim K-S, Kim KD (2019) Complete genome sequence data of Flavobacterium anhuiense strain GSE09, a volatile-producing biocontrol bacterium isolated from cucumber (Cucumis sativus) root. Data Brief 25:104270. https://doi.org/10.1016/j.dib.2019.104270
Kamran S, Shahid I, Baig DN, Rizwan M, Malik KA, Mehnaz S (2017) Contribution of zinc solubilizing bacteria I. growth promotion and zinc content of wheat. Front Microbiol 8:322772. https://doi.org/10.3389/fmicb.2017.02593
Kaneriya JP, Pattani VB, Joshi K, Gandhi D, Sanghvi G (2024) Amelioration of growth of maize (Zea Mays L.) seedling using plant growth promoting bacteria. Plant Sci Today 11(2). https://doi.org/10.14719/pst.3223
Liu J, Zhang J, Zhu M, Wan H, Chen Z, Yang N, Duan J, Wei Z, Hu T, Liu FJA (2022) Effects of plant growth promoting rhizobacteria (PGPR) strain Bacillus licheniformis with Biochar amendment on potato growth and water use efficiency under reduced irrigation regime. J Agron 12(5):1031. https://doi.org/10.3390/agronomy12051031
Lorck H (1948) Production of hydrocyanic acid by bacteria. Physiol Plant 1(2). https://doi.org/10.1111/j.1399-3054.1948.tb07118.x
Napar WPF, Kaleri AR, Ahmed A, Nabi F, Sajid S, Ćosić T et al (2022) The anthocyanin-rich tomato genotype LA-1996 displays superior efficiency of mechanisms of tolerance to salinity and drought. J Plant Physiol 271:153662. https://doi.org/10.1016/B978-0-12-817892-8.00003-9
Parag B, Sasikala C, Ramana CV (2015) Bacillus endolithicus sp. nov., isolated from pebbles. Int J Syst Evol Microbiol 65(Pt 12):4568–4573. https://doi.org/10.1099/ijsem.0.000612
Park YG, Mun BG, Kang SM, Hussain A, Shahzad R, Seo C-W, Kim AY, Lee S-U, Oh KY, Lee DY (2017) Bacillus aryabhattai SRB02 tolerates oxidative and nitrosative stress and promotes the growth of soybean by modulating the production of phytohormones. J PloS One 12(3):e0173203. https://doi.org/10.1371/journal.pone.0173203
Peethambaran B, Hawkins L, Windham GL, Williams WP, Luthe DS (2010) Anti-fungal activity of maize silk proteins and role of chitinases in Aspergillus flavus resistance. Toxin Reviews 29(1):27–39. https://doi.org/10.3109/15569540903402874
Reena (2019) 16S microbial phylogeny of multifunctional plant-growth-promoting rhizobacteria from the rhizosphere of maize (Zea Mays L.) for agricultural soil fortification. BioTechnologia J Biotechnol Comput Biology Bionanotechnology 100(2). https://doi.org/10.5114/bta.2019.85324
Renoud S, Abrouk D, Prigent-Combaret C, Wisniewski-Dyé F, Legendre L, Moënne-Loccoz Y, Muller D (2022) Effect of inoculation level on the impact of the PGPR Azospirillum lipoferum CRT1 on selected microbial functional groups in the rhizosphere of field maize. J Microorganisms 10(2):325. https://doi.org/10.3390/microorganisms10020325
Safiul Azam FM, Lian T, Liang Q, Wang W, Zhang C, Jiang L (2022) Variation of vitamin B contents in maize inbred lines: Potential genetic resources for biofortification. Front Nutr 9:1029119. https://doi.org/10.3389/fnut.2022.1029119
Sahu PK, Singh S, Gupta AR, Gupta A, Singh UB, Manzar N, Bhowmik A, Singh HV, Saxena AK (2020) Endophytic bacilli from medicinal-aromatic perennial Holy basil (Ocimum tenuiflorum L.) modulate plant growth promotion and induced systemic resistance against rhizoctonia Solani in rice (Oryza sativa L.). Biol Control 150:104353. https://doi.org/10.1016/j.biocontrol.2020.10435
Schwyn B, Neilands JJ (1987) Universal chemical assay for the detection and determination of siderophores. Anal Biochem 160(1):47–56.https://doi.org/10.1016/0003-2697(87)90612-9
Shikha B, Manpreet J, Ramanjit K (2019) Nutritive value. In: Akbar H (ed) Maize (pp. Ch. 2). IntechOpen, Rijeka
Shurigin V, Alikulov B, Davranov K, Ismailov ZJ (2022) Bacterial endophytes from halophyte black saxaul (Haloxylon aphyllum Minkw.) and their plant growth-promoting properties. J Appl Biol Biotechnol 10(1):45–53.https://doi.org/10.7324/JABB.2021.100106
Slama HB, Cherif-Silini H, Chenari Bouket A, Qader M, Silini A, Yahiaoui B, Alenezi FN, Luptakova L, Triki MA, Vallat A (2019) Screening for Fusarium antagonistic bacteria from contrasting niches designated the endophyte Bacillus halotolerans as plant warden against fusarium. J Front Microbiol 9:3236. https://doi.org/10.3389/fmicb.2018.03236
Solomon W, Janda T, Molnár Z (2023) Unveiling the significance of rhizosphere: implications for plant growth, stress response, and sustainable agriculture. Plant Physiol Biochem 108290. https://doi.org/10.1016/j.plaphy.2023.108290
Tamura K, Stecher G, Kumar S (2021) MEGA11: Molecular Evolutionary Genetics Analysis Version 11. Mol Biol Evol 38(7):3022–3027. https://doi.org/10.1093/molbev/msab120
Verma SC, Ladha JK, Tripathi AK (2001) Evaluation of plant growth promoting and colonization ability of endophytic diazotrophs from deep water rice. J Biotech 91(2):127–141. https://doi.org/10.1016/S0168-1656(01)00333-9
Wellburn AR (1994) The spectral determination of chlorophylls a and b, as well as total carotenoids, using various solvents with spectrophotometers of different resolution. J Plant Physiol 144(3):307–313
Xu M, Sheng J, Chen L, Men Y, Gan L, Guo S, Shen L (2014) Bacterial community compositions of tomato (Lycopersicum esculentum Mill.) seeds and plant growth promoting activity of ACC deaminase producing Bacillus subtilis (HYT-12-1) on tomato seedlings. World J Microbiol Biotechnol 30:835–845. https://doi.org/10.1007/s11274-013-1486-y
Author Information
Department of Microbiology, Atmiya University, Rajkot, India