Vegetos
(An International Journal of Plant Research & Biotechnology)
Structural analysis and evaluation of anti-complement and α-amylase inhibitory properties of heteroxylan extracted from Salvia aegyptiaca L. seeds
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Research Articles | Published: 09 October, 2024
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Keywords: n Salvia aegyptiacan , Polysaccharides, Characterization, Anti-complement, α-amylase inhibitory
Abstract
In the pursuit of novel bioactive compounds, we employed an ethnobotanical approach to explore the potential of Salvia aegyptiaca L. seeds. Polysaccharides isolated from these seeds named PGSA were subjected to characterize utilizing various techniques such as colorimetric assays, FT-IR spectroscopy, TLC, HPLC, and GC/MS-EI. PGSA exhibited notable content (3.65%), with high total carbohydrate (77.34 ± 0.03%), uronic acid (15.17 ± 0.01%), neutral sugar (63.27 ± 0.33%), proteins (6.93 ± 0.01%), and total phenolic components (11.36 ± 0.01%). Predominantly composed of xylose (93%) with traces of galactose (4%), arabinose (1%), and galacturonic acid (2%). We further explored the effects of PGSA and their partial hydrolysis named PGSAH on anti-complement and α-amylase inhibitory activities. Notably, PGSAH displayed enhanced anti-complement activity (46.69%) compared to PGSA (40%) and approached that of heparin (53.33%) at 1 mg.mL−1. Additionally, PGSAH exhibited significant α-amylase inhibitory potential, with an IC50 of 3.3 mg.mL−1, positioning it between PGSA (5 mg.mL−1) and acarbose (0.3 mg.mL−1). These findings underscore the impact of molecular weight on both biological activities. In conclusion, polysaccharides derived from S. aegyptiaca seeds present promising prospects as novel sources of anti-complement agents and enzyme inhibitors, with potential applications in the food and pharmaceutical industries.
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Author Information
Laboratory for the Protection of Ecosystems in Arid and Semi-Arid Zones, Kasdi Merbah-University, Ouargla, Algeria