Vegetos
(An International Journal of Plant Research & Biotechnology)
Structural analysis and evaluation of anti-complement and α-amylase inhibitory properties of heteroxylan extracted from Salvia aegyptiaca L. seeds
*Article not assigned to an issue yet
Research Articles | Published: 09 October, 2024
First Page: 0
Last Page: 0
Views: 1708
Keywords: n Salvia aegyptiacan , Polysaccharides, Characterization, Anti-complement, α-amylase inhibitory
Abstract
In the pursuit of novel bioactive compounds, we employed an ethnobotanical approach to explore the potential of Salvia aegyptiaca L. seeds. Polysaccharides isolated from these seeds named PGSA were subjected to characterize utilizing various techniques such as colorimetric assays, FT-IR spectroscopy, TLC, HPLC, and GC/MS-EI. PGSA exhibited notable content (3.65%), with high total carbohydrate (77.34 ± 0.03%), uronic acid (15.17 ± 0.01%), neutral sugar (63.27 ± 0.33%), proteins (6.93 ± 0.01%), and total phenolic components (11.36 ± 0.01%). Predominantly composed of xylose (93%) with traces of galactose (4%), arabinose (1%), and galacturonic acid (2%). We further explored the effects of PGSA and their partial hydrolysis named PGSAH on anti-complement and α-amylase inhibitory activities. Notably, PGSAH displayed enhanced anti-complement activity (46.69%) compared to PGSA (40%) and approached that of heparin (53.33%) at 1 mg.mL−1. Additionally, PGSAH exhibited significant α-amylase inhibitory potential, with an IC50 of 3.3 mg.mL−1, positioning it between PGSA (5 mg.mL−1) and acarbose (0.3 mg.mL−1). These findings underscore the impact of molecular weight on both biological activities. In conclusion, polysaccharides derived from S. aegyptiaca seeds present promising prospects as novel sources of anti-complement agents and enzyme inhibitors, with potential applications in the food and pharmaceutical industries.
References
Addoun N, Boual Z, Delattre C, Chouana T, Gardarin C, Dubessay P, al. (2021) Beneficial health potential of algerian polysaccharides extracted from Plantago ciliata Desf. (Septentrional Sahara) leaves and seeds. Appl Sci 11(4299):1–15
Amamou S, Houda Lazreg H, Hafsa J, Majdoub H, Rihouey C, Le Cerf D, Achour L (2020) Effect of extraction condition on the antioxidant, antiglycation and α-amylase inhibitory activities of Opuntia macrorhiza fruit peels polysaccharides. LWT 127:1–8
Bais D, Trevisan A, Lapasin R, Partal P, Gallegos C (2005) Rheological characterization of polysaccharide–surfactant matrices for cosmetic O/W emulsions. J Colloid Interface Sci 290(2):546–556
Barker SA, Bourne EJ, Stacey M, Whiffen DH (1957) Infra-red spectra of carbohydrates. Part I. Some derivatives of D-glucopyranose. J Chem Soc 1:171–176. https://doi.org/10.1039/JR9540000171
Behbahani BA, Fooladi AAI (2012) Shirazi balangu (Lallemantia royleana) seed mucilage: chemical composition, molecular weight, biological activity and its evaluation as edible coating on beefs. Int J Biol Macromol 15(114):882–889. https://doi.org/10.1016/j.ijbiomac.2018.03.177
Belmin J., Chassagne P., Friocourt P., Gonthier R., Jeandel C., Nourhashemi F., & Pfitzenmeyer P. (2016) Gériatrie : pour le praticien. Ed. Elsevier Health Sciences 856p
Bichara LC, Alvarez PE, Bimbi MVF, Vaca H, Gervasi C, Brandán SA (2016) Structural and spectroscopic study of a pectin isolated from citrus peel by using FTIR and FT-Raman spectra and DFT calculations. Infrared Phys Technol 76:315–327
Blondin C, Fischer E, Boisson-Vidal C, Kazatchkine MD, Jozefonvicz J (1994) Inhibition of complement activation by natural sulfated polysaccharides (fucans) from brown seaweed. Mol Immunol 31(4):247–253
Blumenkrantz N, Asboe-Hansen G (1973) New method for quantitative determination of uronic acids. Anal Biochem 54:484–489
Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248–254
Campos BE, Ruivo TD, Da Silva SMR, Madrona GS, Bergamasco RDC (2016) Optimization of the mucilage extraction process from chia seeds and application in ice cream as a stabilizer and emulsifier. LWT-Food Sci Technol 65:874–883
Capek P, Machova E, Turjan J (2009) Scavenging and antioxidant activities of immunomodulating polysaccharides isolated from Salvia officinalis L. Int J Biol Macromol 44(1):75–80
Chakou FZ, Boual Z, Oueld Hadj M D, Belkhalfa H, Bachari K, El Alaoui-Talibi Z and al. (2021) Pharmacological Investigations in Traditional Utilization of Alhagi maurorum Medik in Saharan Algeria: In Vitro Study of Anti Inflammatory and Antihyperglycemic Activities of Water-Soluble Polysaccharides Extracted from the Seeds. Plants. 10(12): 2658.
Chen H, Zhang M, Xie B (2004) Quantification of uronic acids in tea polysaccharide conjugates and their antioxidant properties. J Agric Food Chem 52(11):3333–3336
Chen M, Wu J, Shi S, Chen Y, Wang H, Fan H, Wang S (2016) Structure analysis of a heteropolysaccharide from Taraxacum mongolicum Hand.-Mazz. et anticomplementary activity of its sulfated derivatives. Carbohyd Polym 152:241–252
Chen Y, Li H, Li M, Niu S, Wang J, Shao H, Li T, Wang H (2017) Salvia miltiorrhiza polysaccharide activates T Lymphocytes of cancer patients through activation of TLRs mediated -MAPK and -NF -κB signaling pathways. J Ethnopharmacol 200:165–173
Cheng Y, JIA G, JIANG-SHENG Z, SHAO-PING L, (2010) Use of HPTLC to differentiate among the crude polysaccharides in six traditional Chinese medicines. J Planar Chromatograp 23:46–49
Coimbra MA, Barros A, Rutledge DN, Delgadillo I (1999) FTIR spectroscopy as a tool for the analysis of olive pulp cell-wall polysaccharide extracts. Carbohydr Res 317(1–4):145–154
Correia DM, Dias LG, Veloso ACA, Dias T, Rocha I, Rodrigues LR, Peres AM (2014) Dietary sugar analysis: quantification of fructose oligosaccharides during fermentation by HPLC–RI method. Nutrition Food Sci Technol 1:1–9. https://doi.org/10.3389/fnut.2014.00011
Diallo D, Paulsen BS, Liljebäck TH, Michaelsen TE (2001) Polysaccharides from the roots of Entada Africana Guill et Perr, Mimosaceae, with complement fixing activity. J Ethnopharmacology 74(2):159–171
Drew BT, González-Gallegos JG, Xiang CL, Kriebel R, Drummond CP, Walker JB, Sytsma KJ (2017) Salvia united: The greatest good for the greatest number. Taxon 66:133–145
Du D, Lu Y, Cheng Z, Chen D (2016) Structure characterization of two novel polysaccharides isolated from the spikes of Prunella vulgaris and their anticomplement activities. J Ethnopharmacol S0378–8741(16):30568–30572
Dubois M, Gilles KA, Hamilton JK, Rebers PT, Smith F (1956) Colorimetric method for determination of sugars and related substances. Anal Chem 28:350–356
Frederico I, da Silveira RA, Moreira L, Magalh˜aes B, Claudia do O Pessoa C et al (2021) New properties of chia seed mucilage (Salvia hispanica L.) and potential application in cosmetic and pharmaceutical products. Industrial Crops Prod 171:113981
Ganesan K, Xu B (2019) Anti-diabetic effects and mechanisms of dietary polysaccharides. Molecules 24:1–28
Gao Hw, Zhang M, Liu Y, Xu Q, Yang S (2013) Anticomplement activity of ginsenosides from Panax ginseng. J Functional Foods 5(1):498–502
Geng ZH, Huang L, Song MB, Song YM (2015) Cardiovascular effects in vitro of a polysaccharide from Salvia miltiorrhiza. Carbohyd Polym 12:1241–1247
Ghebregzabeier M, Rufini S, Monaldi B, Lato M (1975) Thin-layer chromatography of carbohydrates. Chromatography 127:133–162
Hammami N, Gara AB, Bargougui K, Ayedi H, Abdalleh FB, Belghith K (2018) Improved in vitro antioxidant and antimicrobial capacities of polysaccharides isolated from Salicornia Arabica. Int J Biol Macromol 120:2123–2130
Hammiche V, Maiza K (2006) Traditional medicine in Central Sahara: Pharmacopoeia of Tassili N’ajjer. J Ethnopharmacol 105:358–367
Hoton-Dorge M (1976) Séparation des aldoses et des polysaccharides par chromatographie sur couche mincede cellulose et nouveau réactif de pulvérisation permettant leur révélation sensible. Chromatography 116:417–423
Hu Z, Jin L, Zong T, Duan Y (2023) Purification, Characterization, and Anticomplementary Activity of Polysaccharides From the Fruits of Amomum tsaoko Crevost & Lemarié. Nat Prod Commun 18(6):1–13
Huo J, Lu Y, Jiao Y, Chen D (2020) Structural characterization and anticomplement activity of an acidic polysaccharide from Hedyotis diffusa. Int J Biol Macromol 155:1553–1560
Jenkins DJ, Wolever TM, Nineham R, Taylor R, Metz GL, Bacon S, Hockaday TD (1978) Guar crispbread in the diabetic diet. Br Med J 2:1744–1746
Jiang YY, Wang L, Zhang L, Wang T, Yu L, Ding CB, Yang RW, Wang XL, Zhou YH (2014) Characterization, antioxidant and antitumor activities of polysaccharides from Salvia miltiorrhiza Bunge. Int J Biol Macromol 70:92–99
Jin W, Zhang W, Liang H, Zhang Q et al (2016) The structure-activity relationship between marine algae polysaccharides and anti-complement activity. Mar Drugs 14(1):3
Kajaria D, Tripathi J, Tripathi YB, Tiwari S (2013) In-vitro α-amylase and glycosidase inhibitory effect of ethanolic extract of antiasthmatic drug-Shirishadi. J Adv Pharm Technol Res 4:206–209
Koocheki A, Mortazavi SA, Shahidi F, Razavi S, Kadkhodaee R, Milani JM (2010) Optimization of mucilage extraction from Qodume shirazi seed (Alyssum homolocarpum) using response surface methodology. J Food Process Eng 33(5):861–882. https://doi.org/10.1016/j.foodhyd.2009.06.014
Kumar A, Lakshman K, Jayaveera K, Shekar S, Swamy N, Khan S, Velumurga C (2011) In vitro α-amylase inhibition and antioxidant activities of methanolic extract of Amaranthus caudatus Linn. Oman Med J 26:166–170
Lee SJ, Lee HS, Kim SY, Shin KS (2018) Immunostimulatory and anti-metastatic activity of polysaccharides isolated from byproducts of the corn starch industry. Carbohyd Polym 181:911–917
Leporrier M. (2011) Petite encyclopédie médicale Hamburger : Guide depratique médicale. 20éme Ed. Lavoisier, 1887p
Ma F, Zhang Y, Liu N, Zhang J, Tan G, Kannan B, Liu X, Bell AE, Tikkanen-kaukanen C (2017) Chemical components and emulsification properties of mucilage from Dioscorea opposita Thunb. Food Chem 228:315–322
Monsigny M, Petit C, Roche AC (1988) Colorimetric determination of neutral sugars by a resorcinol sulfuric acid micromethod. Anal Biochem 175:525–530
Mu˜noz L A, Aguilera J M, Rodriguez-Turienzo L, Cobos A, Diaz O, (2012) Characterization and microstructure of films made from mucilage of Salvia hispanica and whey protein concentrate. J Food Eng 111(3):511–518
Mu˜nozL A, NataliaVera CAM, CarolinaZú˜niga-L´opez B, MauricioMoncada B, Claudia M, Haros C (2021) Physicochemical and functional properties of soluble fiber extracted from two phenotypes of chia (Salvia hispanica L.) seeds. J Food Composit Analysis 104:104138
Naghibi F, Mosaddegh M, Mohammadi Motamed S, Ghorbani A (2005) Labiatae family in folk medicine in Iran: from ethnobotany to pharmacology, Iran. J Pharm Res 2:63–79
Nie C, Zhu P, Wang M, Ma S, Wei Z (2017) Optimization of water-soluble polysaccharides from stem lettuce by response surface methodology and study on its characterization and bioactivities. Int J Biol Macromol 105:912–923
Petera B, Delattre C, Pierre G, Wadouachi ER, Engel E, Poughon L, Michaud P, Fenoradosoa TA (2015) Characterization of arabinogalactan-rich mucilage from Cereus triangularis cladodes. Carbohyd Polym 127:372–380
Pierre G, Graber M, Rafiliposon BA, Dupuy C, Orvain F, De Crignis M, Maugard T (2011) Biochemical composition and changes of extracellular polysaccharides (ECPS) produced during microphytobenthic biofilm development (Marennes-Oléron, France). Microb Ecol 63:157–169
Rjeibi I, Feriani A, Hentati F, Hfaiedh N, Michaud P, Pierre G (2019) Structural characterization of water-soluble polysaccharides from Nitraria retusa fruits and their antioxidant and hypolipidemic activities. Int J Biol Macromol 129:422–432
Rjeibi I, Zaabi R, Jouida W (2020) Characterization of polysaccharides extracted from pulps and seeds of Crataegus azarolus L. var. aronia: Preliminary structure, antioxidant, antibacterial, α-amylase, and acetylcholinesterase inhibition properties. Hindawi Oxidative Med Cellular Longevity 1903056:11
Samuelsen A (2000) Structural features of biologically active polysaccharide fractions from the leaves and seeds of Plantago major L. Springer, Bioactive carbohydrate polymers, pp 37–46
Silva SH, Neves ICO, Oliveira NL, Oliveira ACF, Lago AMT, Giarola TMO, Resende JV (2019) Extraction processes and characterization of the mucilage obtained from green fruits of Pereskia aculeata Miller. Ind Crops Prod 140:111716
Singleton VL, Rossi JA (1965) Colorimetry of total phenolics with phosphomolybdic phosphotungstic acid reagents. Am J Enol Vitic 16:144–158
Song YH, Liu Q, Lv ZP, Chen YY, Zhou YC, Sun XG (2008) Protection of a polysaccharide from Salvia miltiorrhiza. a Chinese medicinal herb, against immunological liver injury in mice. Int J Biol Macromol 43:170–175
Srichamroen A, Thomson ABR, Field CJ, Basu TK (2009) In vitro intestinal glucose uptake is inhibited by galactomannan from Canadian fenugreek seed (Trigonella foenum graecum L.) in genetically lean and obese rats. Nutr Res 29:49–54
Tihomirova K, Dalecka B, Mezule L (2016) Application of conventional HPLC RI technique for sugar analysis in hydrolysed hay. Agronomy Res 14(5):1713–1719
Timilsena YP, Adhikari R, Kasapis S, Adhikari B (2016) Molecular and functional characteristics of purified gum from Australian chia seeds. Carbohydr Polym 136:128–136
Urbizo-Reyes U, San Martin-Gonz´alez M F, Garcia-Bravo J, Malo A L, Liceaga, A M (2019) Physicochemical characteristics of chia seed (Salvia hispanica) protein hydrolysates produced using ultrasonication followed by microwave-assisted hydrolysis. Food Hydrocoll 97:1–10
Wang S, Shi S, Lian H, Zhu C, Wang H, Liu R, Bligh S (2016) Structural Features and Anti-Complement Activity of an Acidic Polysaccharide from Forsythia suspensa. J Glycom Lipidom. 2:1–8
Wei D, Chen T, Yan M et al (2015) Synthesis, characterization, antioxidant activity and neuroprotective effects of selenium polysaccharide from Radix hedysari. Carbohyd Polym 125:161–168
Wu WL, Zhu YT, Zhang L, Yang RW, Zhou YH (2012) Extraction, preliminary structural characterization, and antioxidant activities of polysaccharides from Salvia miltiorrhiza Bunge. Carbohyd Polym 87:1348–1353
Xia L, Li B, Lu Y, Chen D (2019) Structural characterization and anticomplement activity of an acidic polysaccharide containing 3-O-methyl galactose from Juniperus tibetica. Int J Biol Macromol 132:1244–1251
Xia L, Zhu M, Chen D, Lu Y (2020) Juniperus pingii va. wilsonii acidic polysaccharide: Extraction, characterization and anticomplement activity. Carbohyd Polym 231:115728
Xie JH, Jin ML, Morris GA et al (2016) Advances on bioactive polysaccharides from medicinal plants. Crit Rev Food Sci Nutr 56:60–84
Xu H, Zhang Y, Zhang J, Chen D (2007) Isolation and characterization of an anti-complementary polysaccharide D3–S1 from the roots of Bupleurum smithii. Int Immunopharmacol 7:175–182
Yoon NY, Ngo DN, Kim SK (2009) Acetylcholinesterase inhibitory activity of novel chitooligosaccharide derivatives”. Carbohyd Polym 78(4):869–872
Zhang W, Zheng LJ, Zhang ZM, Hai CX (2012) Protective effect of a water -soluble polysaccharide from Salvia miltiorrhiza Bunge on insulin resistance in rats. Carbohyd Polym 89:890–898
Zhao Q, Dong B, Chen J et al (2015) Effect of drying methods on physicochemical properties and antioxidant activities of wolfberry (Lycium barbarum) polysaccharide”. Carbohyd Polym 127:176–181
Zheng H-c, Lu Y, Chen D-f (2018) Anticomplement compounds from Polygonum chinense. Bioorg Med Chem Lett 28(9):1495–1500
Author Information
Laboratory for the Protection of Ecosystems in Arid and Semi-Arid Zones, Kasdi Merbah-University, Ouargla, Algeria