Vegetos
(An International Journal of Plant Research & Biotechnology)
Silver nanoparticles derived from Clerodendrum inerme L. using biosynthesis: an infection curing and enhancing the feasibility of various cell proliferation efficiencies
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Research Articles | Published: 30 July, 2024
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Keywords: Eco-synthesis, Ag NPs, A549, Hep2, HeLa cell lines, Anti-germs activity
Abstract
Biosynthesis-formed silver nanoparticles (Ag NPs) have many biological characteristics and are great substitutes for conventional drugs due to their inexpensive nature. In this work, we synthesized Ag NPs using a suspension from the leaves of the healing herb Clerodendrum inerme L. Multiple methods like XRD, UV–Vis, SEM with EDX, FT-IR, and TEM confirmed the fabricated Ag NPs. Employing the agar well method, the anti-germs properties of bio-synthesized Ag NPs towards pathogenic organisms were assessed. In addition, MTT protocol was used to examine the cytotoxic nature of many cell lines, including A549, HepG2, and HeLa. Using UV–visible spectrum, that which shows an absorption peak at about 400–450 nm, Ag-NPs synthesis was confirmed visually along with a colour shift. AgNPs have an average diameter of 37 nm and are extremely crystalline and spherical, according to SEM examination. With IC50 value of 51.25 μg/mL, synthesized Ag-NPs showed good cell proliferation viability of HeLa cell lines and strong antimicrobial action towards Gram-positive Streptococcus pneumoniae, Staphylococcus aureus, and Gram-negative Enterococcus faecalis, Escherichia coli. As a result, the study s findings demonstrated that eco-synthesized AgNPs had strong cytotoxic and antimicrobial qualities, two essential traits of a molecule with promising therapeutic uses.
References
Akhil R, Rani J, Debnath M (2019) Green synthesis of silver nanoparticles from Tectona grandis seeds extract: characterization and mechanism of antimicrobial action on different microorganisms. J Anal Sci Technol 10(1):5. https://doi.org/10.1186/s40543-018-0163-z
Chhangte V, Samuel L, Ayushi B, Manickam S, Bishwajit C, Samuel LR (2021) Green synthesis of silver nanoparticles using plant extracts and their antimicrobial activities: a review of recent literature. RSC Adv 11(5):2804–2837. https://doi.org/10.1039/d0ra09941d
El-Seedi HR, El-Shabasy RM, Khalifa SAM, Saeed A, Shah A, Shah R, Iftikhar FJ, Abdel-Daim MM, Omri A, Hajrahand NH, Sabir JSM, Zou X, Halabi MF, Sarhan W, Guo W (2019) Metal nanoparticles fabricated by green chemistry using natural extracts: biosynthesis, mechanisms, and applications. RSC Adv 9(42):24539–24559. https://doi.org/10.1039/c9ra02225b
Gabrielyan L, Trchounian A (2019) Antibacterial activities of transient metals nanoparticles and membranous mechanisms of action. World J Microbiol Biotechnol 35(10):162. https://doi.org/10.1007/s11274-019-2742-6
Gomes HIO, Catarina SMM, João AVP (2021) Silver nanoparticles as carriers of anticancer drugs for efficient target treatment of cancer cells. Nanomaterials 11:964. https://doi.org/10.3390/nano11040964
Ibrahim E, Hatem F, Muchen Z, Yang Z, Wen Q, Chengqi Y, Bin L, Jianchu M, Jianping C (2019) Biosynthesis of silver nanoparticles using endophytic bacteria and their role in inhibition of rice pathogenic bacteria and plant growth promotion. RSC Adv 9(50):29293–29299. https://doi.org/10.1039/c9ra04246f
Kenneth S, Denis KB, Charles DK, Eddie MW, Francis E, Jesca LN, Malik M, Juliet S, Edward N, John BK (2021) Green strategy-based synthesis of silver nanoparticles for antibacterial applications. Front Nanotechnol Sec Biomed Nanotechnol 3:697303. https://doi.org/10.3389/fnano.2021.697303
Liu S, Wei L, Hao L, Fang N, Chang MW, Xu R, Yang Y, Chen Y (2009) Sharper and faster “Nano Darts” kill more bacteria: a study of antibacterial activity of individually dispersed pristine single-walled carbon nanotube. ACS Nano 3:3891–3902. https://doi.org/10.1021/nn901252r
Mahadevan S, Vijayakumar S, Arulmozhi P (2018) Phytochemical Analysis and Antimicrobial Activities of Atalantia monophylla (L) Correa and Atalantia racemosa Wight and Arn. Current Bioactive Compounds Rep 14:1–10
Modan EM, Adriana GP (2020) Advantages and disadvantages of chemical methods in the elaboration of nanomaterials. MMS. 43:53–60
Mohamed HEA, Shakeeb A, Ali TK, Dania Z, Javed I, Ikram U, Zabta KS, Malik M (2019) Biosynthesis of silver nanoparticles from Hyphaene thebaica fruits and their in vitro pharmacognostic potential Mater. Res Express 6:1050–1059. https://doi.org/10.1088/2053-1591/ab4217
Mukherjee K, Bhagat N, Kumari M, Arnab RC, Biplab S, Barnali DG (2023) Insight study on synthesis and antibacterial mechanism of silver nanoparticles prepared from indigenous plant source of Jharkhand. J Genet Eng Biotechnol 21:30. https://doi.org/10.1186/s43141-023-00463-3
Peter T, Radka M, Martina C, Zdenka B, Ludmila B, Gabriela T (2023) The role of silver nanoparticles in the diagnosis and treatment of cancer: are there any perspectives for the future? Life (basel). 13(2):466. https://doi.org/10.3390/life13020466
Rebwar FT, Samir MH, Azeez AB, Usame D (2021) Biosynthesis of silver nanoparticles and their applications in harvesting sunlight for solar thermal generation. Nanomaterials 11(9):2421. https://doi.org/10.3390/nano11092421
Saravanan A, Kumar PS, Karishma S, Vo DVN, Jeevanantham S, Yaashikaa PR, George CS (2021) A review on biosynthesis of metal nanoparticles and its environmental applications. Chemosphere 264:128580. https://doi.org/10.1016/j.chemosphere.2020.128580
Suba S, Vijayakumar S, Nilavukkarasi M, Vidhya E, Punitha VN (2022) Eco synthesized silver nanoparticles as a next generation of nanoproduct in multidisciplinary applications. Environmental Chemistry and Ecotoxicology 4:13–19. https://doi.org/10.1016/j.enceco.2021.11.001
Tulinski M, Jurczyk M (2017) Nanomaterials synthesis methods. In: Mansfield E, Kaiser DL, Fujita D, Van de Voorde M (eds) Metrology and Standardization of Nanotechnology. Wiley, Hoboken, NJ, pp 75–98
Vijayakumar S, Rajapriya A, Vidhya E, Nilavukkarasi M, Punitha VN (2023) Agriculture revolution impact on a modest dosage of silver nano-fertilizer: a green strategy. Chem Africa 6:3059–3066. https://doi.org/10.1007/s42250-023-00692-6
Yin IX, Zhang J, Zhao IS, Mei ML, Li Q, Chu CH (2020) The antibacterial mechanism of silver nanoparticles and its application in dentistry. Int J Nanomed 15:2555–2562. https://doi.org/10.2147/IJN.S246764
Author Information
PG and Research Department of Botany, A.V.V.M. Sri Pushpam College (Autonomous), Affiliated to Bharadhidasan University, Poondi, India