Phosphorus solubilizing stress tolerant rhizobacteria for growth promotion of wheat (Triticum aestivum L.)

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Doi: 10.1007/s42535-023-00733-y
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Keywords: Abiotic stress, Bioinoculants, Plant growth promotion, P-solubilization, Wheat


Abstract


Phosphorus is needed by plants for their growth and development as well as different metabolic activities simultaneously being the major yield limiting plant nutrient. The application of the phosphatic fertilizers is the only alternative to prevent the phosphorus deficiency in the plants but the over use of the fertilizers depletes the soil fertility and pollutes the environment. In the present research, 190 bacteria were purified from the rhizosphere of wheat collected from the Keylong region. All the 190 isolates were screened for P-solubilization attribute. Ten selected P-solubilizing strains including EU-KLD-02, EU-KLD-12, EU-KLD-13, EU-KLD-17, EU-KLD-19, EU-KLD-20, EU-KLD-23, EU-KLD-55, EU-KLD-70 and EU-KLD-76 were then further characterized for other attributes of plant growth promotion and tolerance to different abiotic stress. The selected strains were quantified for phosphorus under normal and conditions of abiotic stress. The efficient strains were identified as Serratia marcescens EU-KLD-2, Pseudomonas azotoformans EU-KLD-13, Staphylococcus aureus EU-KLD-23 and Pseudomonas sp. EU-KLD-70. All the ten isolates with multiple PGP attributes were used as bioinoculants in green house experiment on wheat crop. The isolates efficiently improved the studied physiological and growth parameters of wheat. P-solubilizers as biofertilizers are potential candidates to reduce the utilization of chemical fertilizers as well as are a better alternative for environmental sustainability. The present investigation deals with the isolation of P-solubilizing bacteria from rhizospheric soil of wheat, screening for PGP attributes, abiotic stress tolerance and their role in plant growth promotion of wheat,


Abiotic stress, Bioinoculants, Plant growth promotion, P-solubilization, Wheat


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Acknowledgements


The authors are grateful to the Department of Environment, Science & Technology (DEST), Shimla funded project “Development of Microbial Consortium as Bio-inoculants for Drought and Low Temperature Growing Crops for Organic Farming in Himachal Pradesh” for providing the facilities and financial support to undertake the investigations.


Author Information


Gabba Deepika
Department of Microbiology, Akal College of Basic Sciences, Eternal University, Baru Sahib, Sirmour-173101, Himachal Pradesh, India

Sharma Babita
Department of Microbiology, Akal College of Basic Sciences, Eternal University, Baru Sahib, Sirmour-173101, Himachal Pradesh, India


Ramniwas Seema
Department of Biotechnology, University Centre for Research and Development, Chandigarh University, Gharuan-140413, Mohali, India


Kumar Sanjeev
Department of Genetics and Plant Breeding, Faculty of Agriculture Science, GLA University, Mathura, India


Chaubey Kundan Kumar
Division of Research and Innovation, School of Applied and Life Sciences, Uttaranchal University, Dehradun-248007, Uttarakhand, India

Kaur Tanvir
Department of Biotechnology, Dr. Khem Singh Gill Akal College of Agriculture, Eternal University, Baru Sahib, Sirmour-173101, India

Kour Divjot
Department of Microbiology, Akal College of Basic Sciences, Eternal University, Baru Sahib, Sirmour-173101, Himachal Pradesh, India
kourdivjot@gmail.com
,
Yadav Ajar Nath
Department of Biotechnology, Dr. Khem Singh Gill Akal College of Agriculture, Eternal University, Baru Sahib, Sirmour-173101, India

Ahluwalia Amrik Singh
Department of Botany, Akal College of Basic Sciences, Eternal University, Baru Sahib, Sirmour-173101, Himachal Pradesh, India