Nitella mirabilis from Assam: Morphology and Molecular Characterization using RAPD markers

Sarma Bhaswati, Rout Jayashree*, Bhatnagar S. K.1<

Research Articles | Published:

Print ISSN : 0970-4078.
Online ISSN : 2229-4473.
Website:www.vegetosindia.org
Pub Email: contact@vegetosindia.org
Doi: 10.5958/j.2229-4473.27.1.016
First Page: 97
Last Page: 102
Views: 1245


Keywords: Charophyte, <I>Nitella mirabilis</I>, RAPD, Assam, Silchar.


Abstract

Nitella has been observed to grow in different aquatic habitats during algal survey from various areas of Silchar and was taxonomically identified as Nitella mirabilis (O. Nordstedt ex J. Groves) R. D. Wood, 1965 (dioecious). The physico-chemical parameters of water were also analyzed. Using isolated and purified genomic DNA, RAPD band patterns were recorded by PCR amplification for molecular characterization of maacrophytic algae. The electrophoretic patterns were analyzed to provide diagnostic fingerprints for each sample based on RAPD markers. The DNA fingerprint produced consisted of identical RAPD patterns in all the tested samples collected from different localities.


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References


Acknowledgements


Author Information

Sarma Bhaswati Rout Jayashree* Bhatnagar S. K.1<
Department of Ecology and Environmental Science, Assam University, Silchar 788011, Assam, India

1Department of Cell Biology, College of Biotechnology, Sardar Vallabhbhai Patel University of Agriculture & Technology, Meerut-250 110, Uttar Pradesh, India

*Corresponding author E-mail: routjaya@rediffmail.com

Online published on 20 May, 2014.