Nalla Manoj Kumar, Sharma Vinod K., Srivastava Arpita, Mangal Manisha, Rakesh Sharma V., Kumar Mukesh, Pandav Ajay Kumar
Keywords:
CMS, BSA, Sterility, Fertility, Restorer gene
Cytoplasmic male sterility (CMS) is most widely used in the commercial hybrid seed production of chilli and other crop species. It’s a three-line system of hybrid production as it involves a male sterile line, its maintainer and a restorer of fertility for successful hybrid development. Identifying new restorers is much easier in hot pepper as compared to sweet pepper. Almost all the sweet pepper genotypes lack a stable fertility restorer (Rf) allele. Therefore, the identification and development of stable fertility restorer lines with a strong Rf allele is of utmost importance for the commercialization of CMS hybrid production technology in sweet pepper. In the present study, we identified a single dominant gene inheritance pattern of fertility restoration in F2 and backcross populations of sweet pepper lines KTCA5/KTCR15. We also mapped the Rf gene through Bulk segregant analysis of F2 population and based on linkage analysis we identified an SSR marker linked with the male fertility restoration trait.
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We are indebted to Department of science and technology (DST), for providing the fellowship.