Molecular characterization and phylogenetic relationships in Asiatic Vigna using ITS and cpDNA loci

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Vir, Ruchi


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Online ISSN : 2229-4473.
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Doi: 10.1007/s42535-022-00478-0
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Keywords: Asiatic Vignan , ITS1, ITS2, TrnLUAA intron and psba- trn HGUG region, cpDNA loci


Abstract


Asiatic Vigna constitutes group of economically important pulses that are off tremendous agronomic importance. These pulses are not only source of proteins and other nutrients in human diet but also known for increasing soil fertility by nitrogen fixation. However, their inherent low genetic base is the major constraint in the production of improved varieties resulting in low yield. The pulses have increasingly been pushed to marginal lands and therefore, adding in low production. There is an urgent need to explore wild forms for novel genes for the genetic upgradation of cultivated forms, as till now only, exotic lines and cultivated germ plasm has been exploited for the same. Also, it is important to decipher the relationship of wild forms with V. mungo (L.) Hepper and V. radiata (L.) Wilczek. The present study was undertaken to assess genetic diversity and relationship among selected Vigna species belonging to two sub genera of genus Vigna using sequence data of four loci namely ITS1, ITS2, TrnLUAA intron and psbA- trnHGUG region. Comparative phylogeny based on DNA sequence analysis of chloroplast and nuclear loci across the sub genus Ceratatropis has suggested para phyletic origin of sub genus. ITS1 region indicated 70.32% potentially informative sites in comparison to ITS2 in which 66.23% sites were potentially informative. Sequences of psbA – trnHGUG intergenic spacer of cpDNA has 46.37% potentially informative sites, whereas trnLUAA intron has only 10.87%. Cladograms on the basis of ITS 1 are more successful in differentiating groups and have more clearly depicted interspecies relationships in comparison to other three loci namely, ITS 2, trnLUAA intron and psbA-trnH. cpDNA loci are more homoplasious and are less informative than ITS 1 and ITS 2 in Vigna as indicated by values for CI and RI. In comparison to ITS loci, cpDNA loci were inadequate to infer species relationships and differentiation of groups.


Asiatic Vignan                  , ITS1, ITS2, TrnLUAA intron and psba- trn HGUG region, cpDNA loci


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Acknowledgements



Author Information


Vir Ruchi
Department of Botany, Zakir Husain Delhi College, University of Delhi, New Delhi, India
ruchivir@zh.du.ac.in
Jehan Tabassum
Department of Botany, Zakir Husain Delhi College, University of Delhi, New Delhi, India


Bhat K. V.
NRC DNA Fingerprinting, NBPGR, New Delhi, India


Lakhanpaul Suman
Department of Botany, University of Delhi, New Delhi, India