Vegetos
(An International Journal of Plant Research & Biotechnology)
Marker assisted backcross analysis for high temperature tolerance in rice
*Article not assigned to an issue yet
Research Articles | Published: 02 September, 2023
Online ISSN : 2229-4473.
Website:www.vegetosindia.org
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Keywords:
Rice, High temperature tolerance, Recurrent parent genome (RPG) recovery, Background selection
Abstract
Breeding for high temperature tolerant genotypes and varieties in rice may be the solution for sustainable agriculture production in India. In future, the rice production, productivity and cropping season will be significantly decreasing due to high temperature (38 to 40 °C). Heat tolerance at flowering stage of rice is controlled by many Quantitative Trait Locus (QTLs) with small effects. Heat tolerant genotypes can be obtained through introgression of validated high temperature tolerance QTLs from high temperature tolarence genotypes of Nagina 22 (N22). Keeping this in view, the present study was undertaken to introgress two spikelet fertility QTLs viz., qHTSF1.1 and qHTSF4.1 from Nagina 22 (N22) into Improved White Ponni (IWP), a rainfed lowland rice variety. Heat tolerance in rice at flowering stage QTL qHTSF1.1 and qHTSF4.1 were identified as an important source for enhancing spikelet fertility. In BC1F2 {(IWP × (IWP × N22)} population, the maximum recovery of recurrent parent genome (RPG) was recorded to be 79.29% in the plant no. 6-25-29 introgressed with qHTSF1.1. Similarly, plant no. 7-46-18 harbouring qHTSF4.1 also recorded a RPG recovery of 79.04%. There was positive correlation between the yield in BC1F3 and RPG recovery (%) in BC1F2. As the RPG is 79%, more backcross generations with more numbers of markers may be attempted to utilizefine mapping of these QTL regions.
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