Isolation and characterization of endophytic bacteria from medicinal plants (Berberis aristata and Xanthium strumarium) for Rosemary plant growth promotion

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Print ISSN : 0970-4078.
Online ISSN : 2229-4473.
Website:www.vegetosindia.org
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Doi: 10.1007/s42535-024-00826-2
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Keywords: Bioinoculants, Diversity, Plant growth promotion, Rosemary


Abstract


Medicinal plants constitute a diverse range of the endophytic microbes of economical importance. The endophytic microbial communities have a huge potential in promoting the growth and maintaining the health of their host. The present investigation highlights the isolation of endophytic bacteria from the medicinal plants growing around Baru Sahib region and their potential role as bioinoculants to improve the growth of the rosemary plants. In present study, 22 endophytic bacteria from Berberis aristata and Xanthium strumarium were isolated and screened for different plant growth promoting (PGP) attributes of nitrogen fixation, K and P solubilization, and production of indole-3-acetic acid, siderophores, HCN and ammonia. On the basis of PGP attributes, three endophytic bacteria identified using 16S rRNA gene sequencing as Serratia marcescens EU-NLB-03 (N2-fixer), Citrobacter freundii EU-NSB-18 (P-solubilizer) and Bacillus cereus EU-KLB-08 (K-solubilizer) were used as bioinoculants for plant growth promotion of rosemary plants in a pot experiment under green house conditions. The PGP endophytic bacterial strains as consortium and single inoculum positively influenced the growth parameters such as root length, shoot length, fresh and dry weight and physiological parameters such as content of chlorophyll, carotenoids, sugar, phenolic and flavonoids of rosemary plants and could be used as bioinoculants for improving agricultural sustainability. To best of our knowledge, the present study is first report of Serratia marcescens, Citrobacter freundii and Bacillus cereus from Berberis aristata and Xanthium strumarium and their role in plant growth promotion of rosemary plants.


Bioinoculants, Diversity, Plant growth promotion, Rosemary


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Acknowledgements


The authors are grateful to the Department of Environment, Science & Technology (DEST), Shimla funded project “Development of Microbial Consortium as Bio-inoculants for Drought and Low Temperature Growing Crops for Organic Farming in Himachal Pradesh” for providing the facilities and financial support, to undertake the investigations.


Author Information


Sharma Babita
Department of Microbiology, Akal College of Basic Sciences, Eternal University, Baru Sahib, Sirmaur, India

Negi Rajeshwari
Department of Biotechnology, Dr. Khem Singh Gill Akal College of Agriculture, Eternal University, Baru Sahib, Sirmaur, India


Kour Harpreet
Department of Botany, University of Jammu, Jammu, India


Ramniwas Seema
Department of Biotechnology, University Centre for Research and Development, Chandigarh University, Gharuan, Mohali, India


Kumar Sanjeev
Department of Genetics and Plant Breeding, Faculty of Agriculture Science, GLA University, Mathura, India

Rustagi Sarvesh
Depratment of Food Technology, School of Applied and Life Sciences, Uttaranchal University, Dehradun, India

Singh Sangram
Department of Biochemistry, Dr. Ram Manohar Lohia Avadh University, Faizabad, India

,
Kour Divjot
Department of Microbiology, Akal College of Basic Sciences, Eternal University, Baru Sahib, Sirmaur, India
kourdivjot@gmail.com
Negi Rajeshwari
Department of Biotechnology, Dr. Khem Singh Gill Akal College of Agriculture, Eternal University, Baru Sahib, Sirmaur, India

Kour Harpreet
Department of Botany, University of Jammu, Jammu, India