Vegetos
(An International Journal of Plant Research & Biotechnology)
In vitro multiplication of Rambutan (Nephilium lappaceum L.) cv. Arka Coorg Arun through nodal segments
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Bhattacharjee Panchaal, Aswath C., Hariyabbe Nataraja Kendagoli, Rime Jome, Das Shubranil
Research Articles | Published: 31 August, 2024
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Keywords: Arka Coorg Arun, In vitro, Micropropagation, Rambutan, Woody plant media
Abstract
Tropical fruits serve as vital pillars for health promotion, economic support, and cultural enrichment across diverse regions due to their array of flavors and nutritional advantages. This research endeavors to furnish critical insights and optimal conditions for the in vitro propagation of Rambutan (Nephelium lappaceum L.) cv. Arka Coorg Arun. Through meticulous micro propagation studies, it was observed that single nodal segments from laboratory-grown current-season seedlings and one-year-old seedlings exhibited significantly elevated survival rates at 46.67% and 43.33%, respectively. Employing carbendazim 50 WP, gentamicin, and mercuric chloride for surface sterilization led to a notable decrease in contamination rates (31.67%) alongside an augmented survival rate (61.67%). Most effective treatment in mitigating phenolic browning (10.00%) and achieving a robust survival rate (85.55%) involved ascorbic acid, 0.5% PVP, and 2% sucrose. For shoot initiation, optimal medium was identified as WPM + BAP (2 mg/L) for nodal explants from both seedling types, showcasing survival rates of 91.67% and 86.67% and a swift initiation period of 9.33 and 9.00 days, respectively. Additionally, GA3 at 2 mg/L significantly stimulated shoot elongation, yielding heightened shoot length (3.18 cm) and maximum mean shoot diameter (0.46 cm). In vitro rooting achieved its greatest success with MS + IBA (4 mg/L) + IAA (4 mg/L), demonstrating a peak rooting percentage (78.33%) and a shorter duration for root initiation (38.67 days). The subsequent hardening of rooted plantlets through transplantation into plastic cups filled with coco peat culminated in successful adaptation. These advancements not only bolster the sustainable cultivation of rambutan but also align with broader objectives, including the promotion of agricultural productivity, conservation of genetic diversity, and assurance of food security in tropical fruit-producing regions.
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Author Information
Department of Fruit Science, K.R.C. College of Horticulture, Arabhavi, University of Horticultural Sciences, Bagalkot, India