Vegetos
(An International Journal of Plant Research & Biotechnology)
HR-LCMS based metabolite profiling and evaluation of the antioxidant potential of Paspalum fimbriatum Kunth.
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Short Communications | Published: 10 January, 2025
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Keywords: n Paspalum fimbriatumn , HR-LCMS, Bioactive phytoconstituents, Antioxidant
Abstract
Paspalum fimbriatum Kunth. (Family: Poaceae) is an ethnomedicinal plant used traditionally to treat different disorders, including diabetes, helminth infections, etc. This study aimed to determine the total phenolic content (TPC), total flavonoid content (TFC), antioxidant activity, and characterisation of phytochemicals present in the plant. Ethanol extract of the whole plant of Paspalum fimbriatum and its fractions were analysed for the presence of TPC and TFC by Folin–Ciocalteu and aluminium chloride colourimetric method, respectively. Nitric oxide (NO) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity were assessed for the antioxidant properties. The HR-LCMS technique was used for phytochemical profiling. Qualitative phytochemical studies highlighted the presence of various phytoconstituents like alkaloids, flavonoids, glycosides, tannins, steroids, phenols and terpenoids. Preliminary screening indicated significant efficacy of ethyl acetate fraction as a potent antioxidant agent, accompanied by a conspicuous amount of TPC (64.9 ± 0.57 mg GAE/g of dry material) and TFC (26.2 ± 0.23 mg QE/g of dry material). Among the extract and fractions, the ethyl acetate fraction showed the highest antioxidant activity with potent DPPH (IC50 = 14.5 ± 0.27 µg/mL) and NO radical scavenging properties (IC50 = 25.7 ± 0.19 µg/mL). Thus, the ethyl acetate fraction was subsequently analysed to enumerate its chemical constituents using the HR-LCMS technique. The HR-LCMS profiling of ethyl acetate fraction indicated the presence of forty-four phytoconstituents belonging to various classes of compounds. Moschamine, gabapentin, sphinganine, (+)-epicatechin, (e)-1-(2-octenyl) cyclopentanone, and cimifugin are some of the major identified compounds having antioxidant capacity. The present study highlights the possible bioactivity of the plant.
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Author Information
Faculty of Pharmaceutical Science, Assam down town University, Guwahati, India