Green synthesis of antimicrobial selenium and gold nanoparticles using Aegle marmelos fruit extract

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Research Articles | Published:

Print ISSN : 0970-4078.
Online ISSN : 2229-4473.
Website:www.vegetosindia.org
Pub Email: contact@vegetosindia.org
Doi: 10.1007/s42535-022-00469-1
First Page: 971
Last Page: 979
Views: 505


Keywords: Selenium nanoparticle, Gold nanoparticle, Aegle marmelos , Antibacterial activity, Pathogens


Abstract


Nanoparticles of selenium and gold when synthesized through the green route using aqueous plant extract, exhibit strong antimicrobial activities with minimum or no toxicity. Aegle marmelos (L) Correa, an ethnobotanically well-known fruit for its wide medicinal values, has been utilized in this work for the synthesis of both, selenium and gold nanoparticles. The synthesis of the nanoparticles was first detected by the change in colour and formation of colloids in the reaction solution, followed by UV–visible spectroscopy. The λmax of selenium and gold nanoparticles were found at 333 nm and 552 nm, respectively. The XRD spectrum confirms the pure crystalline nature of the nanoparticles. Scanning electron microscopic image revealed their shape as mostly spherical, however, few of the gold nanoparticles were found to be triangular. Both the nanoparticles have shown significantly strong antibacterial activity against the tested pathogens (Micrococcus luteus, Klebsiella pneumoniae, Staphylococcus epidermidis, Pseudomonas aeruginosa, and Staphylococcus aureus) and the best activity has been observed in S. epidermidis for both SeNP and AuNP.


Selenium nanoparticle, Gold nanoparticle, 
              Aegle marmelos
            , Antibacterial activity, Pathogens


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References


Adewale OB, Egbeyemi KA, Onwuelu JO et al (2020) Biological synthesis of gold and silver nanoparticles using leaf extracts of Crassocephalum rubens and their comparative in vitro antioxidant activities. Heliyon 6(11):e05501. https://doi.org/10.1016/j.heliyon.2020.e05501


Alam H, Khatoon N, Raza M, Ghosh PC, Sardar M (2019) Synthesis and characterization of nano selenium using plant biomolecules and their potential applications. BioNanoScience 9(1):96–104. https://doi.org/10.1007/s12668-018-0569-5


Banerjee P, Nath D (2015) A phytochemical approach to synthesize silver nanoparticles for non-toxic biomedical application and study on their antibacterial efficacy. NanoSci Technol 2(1): 1–14. https://doi.org/10.15226/2374-8141/2/1/00123


Bisht B, Pancholi D, Pande V et al (2021) A green approach to synthesize Au nanoplates using Morus indica L. fruit extract and their superior activities in catalysis and surface enhanced Raman scattering. Vegetos. https://doi.org/10.1007/s42535-021-00245-7


Chang CC, Yang MH, Wen HM, Chern JC (2002) Estimation of total flavonoid content in propolis by two complementary colorimetric methods. J Food Drug Anal 10(3): 178–182. https://doi.org/10.38212/2224-6614.2748


Cittrarasu V, Kaliannan D, Dharman K et al (2021) Green synthesis of selenium nanoparticles mediated from Ceropegia bulbosa Roxb extract and its cytotoxicity, antimicrobial, mosquitocidal and photocatalytic activities. Sci Rep 11(1):1–15. https://doi.org/10.1038/s41598-020-80327-9


Deepa B, Ganesan V (2015) Bioinspiredsynthesis of selenium nanoparticles using flowers of Catharanthus roseus (L.) G. Don. and Peltophorum pterocarpum (DC.) Backer ex Heyne–a comparison. Int J ChemTech Res 7:725–733


Devi M, Devi S, Sharma V, Rana N, Bhatia RK, Bhatt AK (2020) Green synthesis of silver nanoparticles using methanolic fruit extract of Aegle marmelos and their antimicrobial potential against human bacterial pathogens. J Tradit Complement Med 10(2):158–165. https://doi.org/10.1016/j.jtcme.2019.04.007


Durán N, Durán M, de Jesus MB, Seabra AB, Fávaro WJ, Nakazato G (2016) Silver nanoparticles: a new view on mechanistic aspects on antimicrobial activity. Nanomedicine 12(3):789–799. https://doi.org/10.1016/j.nano.2015.11.016


ElMitwalli OS, Barakat OA, Daoud RM et al (2020) Green synthesis of gold nanoparticles using cinnamon bark extract, characterization, and fluorescence activity in Au/eosin Y assemblies. J Nanopart Res 22:309. https://doi.org/10.1007/s11051-020-04983-8


Fardsadegh B, Malmiri HJ (2019) Aloe vera leaf extract mediated green synthesis of selenium nanoparticles and assessment of their in vitro antimicrobial activity against spoilage fungi and pathogenic bacteria strains. Green Process Synth 8(1):399–407. https://doi.org/10.1515/gps-2019-0007


Ferro C, Florindo HF, Santos HA (2021) Selenium nanoparticles for biomedical applications: from development and characterization to therapeutics. Adv Healthcare Mater 10(16):2100598. https://doi.org/10.1002/adhm.202100598


Filipović N, Ušjak D, Milenković MT, Zheng K, Liverani L, Boccaccini AR, Stevanović MM (2021) Comparative study of the antimicrobial activity of selenium nanoparticles with different surface chemistry and structure. Fron Bioeng Biotechnol 8:624621. https://doi.org/10.3389/fbioe.2020.624621


Hazra SK, Sarkar T, Salauddin M, Sheikh HI, Pati S, Chakraborty R (2020) Characterization of phytochemicals, minerals and in vitro medicinal activities of bael (Aegle marmelos L.) pulp and differently dried edible leathers. Heliyon 6(10):e05382. https://doi.org/10.1016/j.heliyon.2020.e05382


Hu X, Zhang Y, Ding T, Liu J, Zhao H (2020) Multifunctional gold nanoparticles: a novel nanomaterial for various medical applications and biological activities. Front Bioeng Biotechnol 8:990. https://doi.org/10.3389/fbioe.2020.00990


Kalita C, Sarkar RD, Verma V et al (2021) Bayesian modeling coherenced green synthesis of NiO nanoparticles using Camellia sinensis for efficient antimicrobial activity. BioNanoScience 11:825–837. https://doi.org/10.1007/s12668-021-00882-x


Kamnev AA, Dyatlova YA, Kenzhegulov OA, Vladimirova AA, Mamchenkova PV, Tugarova AV (2021) Fourier transform infrared (FTIR) spectroscopic analyses of microbiological samples and biogenic selenium nanoparticles of microbial origin: sample preparation effects. Molecules 26(4):1146. https://doi.org/10.3390/molecules26041146


Kumar A, Rabha J, Jha DK (2021) Antagonistic activity of lipopeptide-biosurfactant producing Bacillus subtilis AKP, against Colletotrichum capsici, the causal organism of anthracnose disease of chilli. Biocatal Agric Biotechnol 36:102133. https://doi.org/10.1016/j.bcab.2021.102133


Kumaran CKS, Agilan S, Velauthapillai D et al (2011) Synthesis and characterization of selenium nanowires. ISRN Nanotechnol. https://doi.org/10.5402/2011/589073


Logeswari P, Silambarasan S, Abraham J (2015) Synthesis of silver nanoparticles using plants extract and analysis of their antimicrobial property. J Saudi Chem Soc 19(3):311–317. https://doi.org/10.1016/j.jscs.2012.04.007


Mohamad NAN, Arham NA, Jai J, Hadi A (2014) Plant extract as reducing agent in synthesis of metallic nanoparticles: a review. Adv Mater Res Trans Tech Pub Ltd 832:350–355


Nayak V, Singh KR, Singh AK, Singh RP (2021) Potentialities of selenium nanoparticles in biomedical science. New J Chem 45(6):2849–2878. https://doi.org/10.1039/D0NJ05884J


Pradeep M, Kruszka D, Kachlicki P, Mondal D, Franklin G (2021) Uncovering the phytochemical basis and the mechanism of plant extract-mediated eco-friendly synthesis of silver nanoparticles using ultra-performance liquid chromatography coupled with a photodiode array and high-resolution mass spectrometry. ACS Sustain Chem Eng 10(1):562–571. https://doi.org/10.1021/acssuschemeng.1c06960


Qais FA, Shafiq A, Khan HM et al (2019) Antibacterial effect of silver nanoparticles synthesized using Murraya koenigii (L.) against multidrug-resistant pathogens. Bioinorg Chem Appl. https://doi.org/10.1155/2019/4649506


Ramamurthy C, Sampath KS, Arunkumar P et al (2013) Green synthesis and characterization of selenium nanoparticles and its augmented cytotoxicity with doxorubicin on cancer cells. Bioproc Biosyst Eng 36:1131–1139. https://doi.org/10.1007/s00449-012-0867-1


Rao PV, Nallappan D, Madhavi K, Rahman S, Jun Wei L, Gan SH (2016) Phytochemicals and biogenic metallic nanoparticles as anticancer agents. Oxid Med Cell Longev. https://doi.org/10.1155/2016/3685671


Ren X, Song Y, Liu A, Zhang J, Yang P, Zhang J, An M (2015) Experimental and theoretical studies of DMH as a complexing agent for a cyanide-free gold electroplating electrolyte. RSC Adv 5(80):64997–65004. https://doi.org/10.1039/C5RA13140E


Sarkar RD, Singh HB, Kalita MC (2021) Enhanced lipid accumulation in microalgae through nanoparticle-mediated approach, for biodiesel production: a mini-review. Heliyon. https://doi.org/10.1016/j.heliyon.2021.e08057


Sarkar RD, Lahkar P, Kalita MC (2022) Glycosmis pentaphylla (Retz.) DC leaf extract mediated synthesis of selenium nanoparticle and investigation of its antibacterial activity against urinary tract pathogens. Bioresource Technol Rep 17:100894. https://doi.org/10.1016/j.biteb.2021.100894


Shah R, Vaghela H, Pathan A (2020) Synthesis and characterization of biogenic gold nanoparticles using Aegle marmelos extracts: antibacterial assay. Biosci Biotechnol Res Commun 13(1):307–312. https://doi.org/10.33263/BRIAC112.96199628


Shaikh S, Nazam N, Rizvi SMD, Ahmad K, Baig MH, Lee EJ, Choi I (2019) Mechanistic insights into the antimicrobial actions of metallic nanoparticles and their implications for multidrug resistance. Int J Mol Sci 20(10):2468. https://doi.org/10.3390/ijms20102468


Shaikh JR, Patil MK (2020) Qualitative tests for preliminary phytochemical screening: An overview. Int J Chem Stud 8(2): 603–608. https://doi.org/10.22271/chemi.2020.v8.i2i.8834


Sharma G, Sharma AR, Bhavesh R et al (2014) Biomolecule-mediated synthesis of selenium nanoparticles using dried Vitis vinifera (raisin) extract. Molecules 19(3):2761–2770. https://doi.org/10.3390/molecules19032761


Siddiqi KS, Husen A (2017) Recent advances in plant-mediated engineered gold nanoparticles and their application in biological system. J Trace Elem Med Biol 40:10–23. https://doi.org/10.1016/j.jtemb.2016.11.012


Slinkard K, Singleton VL (1977) Total phenol analysis: automation and comparison with manual methods. Am J Enol Vitic 28:49–55


Sondi I, Salopek-Sondi B (2004) Silver nanoparticles as antimicrobial agent: a case study on E. coli as a model for Gram-negative bacteria. J Colloid Interface Sci 275(1):177–182. https://doi.org/10.1016/j.jcis.2004.02.012


Vyas J, Rana S (2017) Antioxidant activity and biogenic synthesis of selenium nanoparticles using the leaf extract of Aloe vera. Int J Curr Pharm Res 9(4):147–152. https://doi.org/10.22159/ijcpr.2017v9i4.20981


Wang L, Hu C, Shao L (2017) The antimicrobial activity of nanoparticles: present situation and prospects for the future. Int J Nanomed 12:1227–1249. https://doi.org/10.2147/ijn.s121956


Xin Lee K, Shameli K, Miyake M et al (2016) Green synthesis of gold nanoparticles using aqueous extract of Garcinia mangostana fruit peels. J Nanomater 2:1–7. https://doi.org/10.1155/2016/8489094


Yu Z, Li Q, Wang J, Yu Y, Wang Y, Zhou Q, Li P (2020) Reactive oxygen species-related nanoparticle toxicity in the biomedical field. Nanoscale Res Lett 15(1):1–14. https://doi.org/10.1186/s11671-020-03344-7

 


Acknowledgements


The authors would like to acknowledge the Department of Biotechnology, Botany, Chemistry, and Instrumentation & USIC (SAIF), Gauhati University; and Guwahati Biotech Park, Assam, India for providing the technical and analytical facilities in conducting the research work.


Author Information


Sarkar Rajesh Dev
Department of Biotechnology, Gauhati University, Guwahati, India

Mena Sushmita
Department of Microbiology, Central University of Tamil Nadu, Thiruvarur, India


Kumar Amrit
Department of Botany, Gauhati University, Guwahati, India


Sharma Rantumoni
Department of Botany, Gauhati University, Guwahati, India


Nath Namita
Department of Botany, Gauhati University, Guwahati, India

Jha Dhruva Kumar
Department of Botany, Gauhati University, Guwahati, India

Kalita Mohan Chandra
Department of Biotechnology, Gauhati University, Guwahati, India
mckalita@gauhati.ac.in