Exploring the antifungal properties of tannin extracts from Olea europaea var. rougette leaves against Aspergillus spp.

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Print ISSN : 0970-4078.
Online ISSN : 2229-4473.
Website:www.vegetosindia.org
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Doi: 10.1007/s42535-024-00830-6
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Keywords: n Olea europaea var. rougetten , Antifungal, Tannins, n Aspergillusn , Germination inhibition, Chromatography


Abstract


Due to significant health risks associated with chemical antifungal agents in food storage, safe and natural sources are now one of the most frequently demanded ingredient for food preservation. Byproducts from olive trees provide an affordable and sustainable source of bioactive chemicals. This study will examine the mycelial growth and germination-inhibitory properties of Olea europaea var. rougette leaves and characterize their phytochemical makeup.The phytochemical screening revealed the presence of polyphenolics compounds mainly tanins.The chemical composition was analyzed by gas chromatography mass spectrometry (GCMS). Total polyphenolic content (TPC), hydrolysable tannins content (HTC) and condensed tannin content (CTC) were assessed using Foilin ciocalteu, potassium iodide and vanilline methods, respectively. The mycelial growth and germination inhibition properties were examined on soft wheat based solid and liquid media. Tannic extract (ethyl acetate extract) revealed highest amounts in polyphenols, hydrolysable tanins and condensed. GC–MS profile of tannic extract showed the presence of 26 bioactive molecules known for their antifungal potential, mainly: Guaiacol (0.12%), 2-methoxy-4-vinylphenol (1.52%), Syringol (0.18%), p-tyrosol (4.70%), homovanillyl alcohol (1.32%), 4-propylresorcinol (1.07%), β-camphor (0.17%), β-turmerone (1.57%) and 4-phenyl-quinolin-2-ol (6.58%). The tannic extract (ethyl acetate fraction) showed significant mycelial growth and germination inhibition activities in contrast to hydro-acetonic and dichloromethanic extracts. Furthermore, myelial growth inhibition showed more effectiveness in comparison to germination inhibition technique.The results suggested that byproducts of Olea europaea var. rougette leaves are a substantial natural source of bioactive compounds, especially tannins with potent antifungal characteristics in vitro which make it interesting to investigate its effectiveness in vivo.


n                     Olea europaea var. rougetten                  , Antifungal, Tannins, n                     Aspergillusn                  , Germination inhibition, Chromatography


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Acknowledgements


The Algerian Ministry of Higher Education and Scientific Research (MERS), General Directorate of scientific research and technological development (DGRSDT), Ibtikar Program and the University of Boumerdes, Algeria, supported this research. (PRFU Project, Code: D00L05UN350120180003). The authors would like to thank Pr Bahidj N. director and Mr Snoussaoui H. head engineer of the laboratory of Laboratory of soft technologies,valorization, physicochemistry of biological materials and biodiversity (LTDVPMBB), Faculty of Science, University of M’hmed Bougara of Boumerdes, 35000, Boumerdes, Algeria.


Author Information


Bennacer Amel
Laboratory of Valorization and Conservation of Biological Ressources (VALCORE), Department of Biology, Faculty of Sciences, University of M’Hamed Bougara, Boumerdes, Algeria
a.bennacer@univ-boumerdes.dz
Sahir-Halouane Fatma
Laboratory of Valorization and Conservation of Biological Ressources (VALCORE), Department of Biology, Faculty of Sciences, University of M’Hamed Bougara, Boumerdes, Algeria


Aitslimane-Aitkaki Sabrina
Laboratory of Valorization and Conservation of Biological Ressources (VALCORE), Department of Biology, Faculty of Sciences, University of M’Hamed Bougara, Boumerdes, Algeria


Tihar-Benzina Farida
Laboratory of Valorization and Conservation of Biological Ressources (VALCORE), Department of Biology, Faculty of Sciences, University of M’Hamed Bougara, Boumerdes, Algeria


Oukali Zahia
Laboratory of Valorization and Conservation of Biological Ressources (VALCORE), Department of Biology, Faculty of Sciences, University of M’Hamed Bougara, Boumerdes, Algeria

Oliveira Ivo Vaz
Centre for the Research and Technology of Agro-Environmental and Biological Sciences - CITAB, Inov4Agro -Institute for Innovation, Capacity Building and Sustainability of Agri-Food Production, University of Trasos-Montes and Alto Douro (UTAD), Vila Real, Portugal

Rahmouni Naima
Faculté des Sciences de la Nature et de la Vie, University of Batna, Batna, Algeria