Vegetos
(An International Journal of Plant Research & Biotechnology)
Effect of plant growth regulators on callus induction in Solanum torvum SW: a medicinal plant
*Article not assigned to an issue yet
Research Articles | Published: 08 September, 2023
Online ISSN : 2229-4473.
Website:www.vegetosindia.org
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Keywords:
Cotyledon, Hypocotyl, Leaf explants, Callus, 2,4-D, NAA, IAA, n Solanum torvumn
Abstract
The present study reports on the effect of various plant growth regulators (PGRs) on callus induction from different types of explants of Turkey Berry (Solanum torvum SW), an ethno-medicinal plant. The explants viz., cotyledon, hypocotyl and leaf were cultured on MS medium fortified with different concentrations (1.0–6.0 mg/L) of auxins (2,4-D, NAA and IAA) alone. The highest percentage of responding cultures from leaf (95% at 4.0 mg/L NAA, 94% at 4.0 mg/L IAA and 91% at 5.0 mg/L IAA, respectively) followed by cotyledon (90% at 4.0 mg/L NAA, 88% at 4.0 mg/L IAA and 84% at 5.0 mg/L IAA, respectively) and hypocotyl (80% at 4.0 mg/L NAA, 80% at 4.0 mg/L IAA and 76% at 5.0 mg/L IAA, respectively) were found. More amount of callus formation was also observed from all the three explants at 2–4 mg/L NAA, 3–5 mg/L IAA and 4–5 mg/L 2,4-D. NAA was found to be the potent PGR for callus induction and proliferation followed by IAA and 2,4-D in S. torvum. Faster callus proliferation and very high yield of callus mass were produced within 4 weeks on MS medium augmented with NAA followed by IAA and 2,4-D. Callusing ability was found to be more in leaf cultures followed by cotyledon and hypocotyl explants. Since it is medicinally important, the protocol developed for the induction of callus can be used for isolation of pharmaceutically important secondary metabolites in S. torvum.
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References
Agrawal AD, Bajpei PS, Patil AA, Bavaskar SR (2010) Solanum torvum Sw. phytopharmacological review. Sch Res Lib 2(4):403–407
Alim MA, Biswas BK, Hasanuzzaman M, Baka P, Roy UK (2014) Callus induction of brinjal by genotype and growth regulators. J Expt Biosci 5:35–42
Arthan D, Svasti J, Kittakoop P, Pittayakhachonwut D, Tanticharoen M, Thebtaranonth Y (2002) Antiviral isoflavonoid sulphate and steroidal glycosides from the fruits of Solanum torvum. Phytochemistry 59(40):459–463
Bhakuni DS, Dhar ML, Dhar MM, Dhawan BN, Mehrotra BN (1969) Screening of indian plants for biological activity: part II. Indian J Exp Biol 7:250–262
Biswas A, Roy A, Miah MAB, Bhadra SK (2007) Plant tissue culture. Biotechnol 217:59–64
Collonnier C, Fock I, Kashyap V, Rotino GL, Daunay MC, Lian Y, Mariska IK, Rajam MV, Servaes A, Ducreux G, Sinhachakr D (2001) Applications of biotechnology in Egg Plant. Plant Cell Tiss Org Cult 65:91–107
Deb D, Sarkar A, Deb Barma B, Datta BK, Majumda K (2013) Wild edible plants and their utilization in traditional recipes of Tripura, Northeast India. Adv Biol Res 7(5):203–211
Ewais EA, Desouky SA, Eshazly EH (2015) Studies on callus induction, phytochemical constituents and antimicrobial activity of Solanum nigrum L. (Solanaceae). Nat Sci 13:133–138
Friedman M, Lee KR, Kim HJ, Lee IS, Kozukue N (2005) Anticarcinogenic effects of glycoalkaloids from potatoes against human cervical, liver, lymphoma, and stomach cancer cells. J Agric Food Chem 53:6162–6169
Ghan Singh M, Rajinikanth M, Rama Swamy N (2021) Effect of plant growth regulators on somatic embryogenesis and plantlet development of turkey berry (Solanum torvum SW). Europ J Medi Plants 32(7):1–8
Govindarajan P, Chinnachamy C (2014) Phytochemical and therapeutic evaluation of leaf and in vitro derived callus and shoot of Solanum trilobatum. Pak J Pharm Sci 27:2101–2107
Hoque A, Islam R, Arima S (2000) High frequency plant regeneration from cotyledon derived callus of Mimordica dioica (Roxb.) Willd. Phytomorphology 50:267–272
Jacqueline TB, Charlwood VB (1986) The control of callus formation and differentiation in scented pelargoniums. J Plant Physiol 123:409–417
Lee KR, Kozukue N, Han JS, Park JH, Chang EY, Baek EJ, Chang JS, Friedman M (2004) Glycoalkaloids and metabolites inhibit the growth of human colon (HT29) and liver (HepG2) cancer cells. J Agric Food Chem 52:2832–2839
Mahadev MD, Panathula CS, Naidu CV (2014) Efficient protocol for in vitro callus induction and indirect plant regeneration of Solanum viarum (Dunal): an important anticancer medicinal plant. Int J Med Aromat Plants 4:117–123
Maiti PC, Mookherjea S, Matew R, Dan SS (1979) Studies on indian Solanum I. Alkaloid. Content and detection of Solasodine. Econ Bot 33:75–77
Mathur S, Shekhawat GS (2013) Establishment and characterization of Stevia rebaudiana (Bertoni) cell suspension culture: an in vitro approach for production of stevioside. Acta Physiol Plant 35:931–939
Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassay with tobacco tissue culture. Physiol Plant 159:473–497
Omar MS (1988) Rhazya stricta Decaisne: in vitro culture and the production of indole alkaloids. In: Bajaj YPS (ed) Biotechnology in agriculture and forestry. Springer Berlin Heidelberg, New York, pp 529–540
Praveen M, Lakshman A, Ugandhar T, Ramaswamy N (2001) Callusing efficiency and plant regeneration from different explants of Strychnos potatorum—a medicinally important forest tree. In: Sadanandam A, Reddy KJM, Reddy SR, Rama Swamy N (eds) Proc. on frontiers of plant biotechnology, pp 183–197
Priyanka A, Yogananth N, Ali MS, Anuradha V (2014) Antibacterial and antifungal activity of Solanum torvum Sw. An ethnomedicinal plant. Int J Compr Res Biol Sci 1(3):6–12
Rama Swamy N, Ugandhar T, Praveen M, Lakshman A, Rambabu M, Venkataiah P (2004) In vitro propagation of medicinally important Solanum surattense. Phytomorphology 54:281–289
Sarin R (2005) Useful metabolites from plant tissue cultures. Biotechnology 4:79–93
Sharada D, Sai Krishna P, Rama Swamy N (2019) Plant regeneration via somatic embryogenesis in Solanum nigrum L. (Black nightshade) (Solanaceae). Biotechnol J Int 23(1):1–9
Silva TMS, Batista MM, Camara CA, Agra MF (2005) Molluscicidal activity of some brazilian Solanum spp. (Solanaceae) against Biomphalaria glabrata. Ann Trop Med Parasitol 99:419–425
Smith SW, Giesbrecht E, Thompson M, Nelson LS, Hoffman RS (2008) Solanaceous steroidal glycoalkaloids and poisoning by Solanum torvum, the normally edible susumber berry. Toxicon 52(6):667–676
Solouki M, Hoshyar H, Ramroudi M, Tavassoli A (2011) Comparison and evaluation of steroid alkaloid solasodine on in vivo and in vitro cultures of Solanum surayyense burm. L. Afr J Microbiol Res 5:3810–3814
Sundari SG, Rekha S, Parvathi A (2015) Phytochemical evaluation of three species Solanum L. Int J Res Ayurveda Pharm 4:420–435
Tejavathi DH, Bhuvana B (1998) In vitro morphogenetic studies in Solanum viarum Dunal. J Swamy Bot Cl 15:27–30
Zou LJ, Yang JT, Wu QG (2017) A protocol for rapid and high frequency in vitro propagation of Solanum nigrum L. Sains Malays 46:1183–1189
Acknowledgements
We thank the University Grants Commission, New Delhi, India for providing the financial assistance under Rajiv Gandhi National Fellowship as JRF/SRF (Ref. No. F.14 − 2(SC/ST)/2009 (SA-III).
Author Information
Department of Botany, Government Degree and PG College, Eturnagaram, Mulugu District, India