Vegetos
(An International Journal of Plant Research & Biotechnology)
Effect of lighting spectrums in the induction of PLBs in Prosthechea fragans (Sw.) W.E. Higgins (Orchidaceae)
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Cervantes-Rodríguez Brandon Giovanni, Iglesias-Andreu Lourdes Georgina
Research Articles | Published: 31 December, 2024
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Keywords: Protocorms, Zeatin, Red LED light, Orchids
Abstract
In this study, we developed a protocol to induce PLBs of Prosthechea fragans (Sw.) W.E. Higgins through in vitro culture. For this purpose, three-month-old P. fragans protocorms were used and inoculated on Murashige & Skoog (MS) basal medium with 500 mg L− 1 of hydrolyzed casein (CH) and different Zeatin (Z) concentrations (0, 0.25, 0.40, 0.50, 0.60, and 0.75 mg L− 1). In vitro, cultured protocorms were exposed to two light conditions: fluorescent light and red LED light. The results showed that protocorms grown on MS medium supplemented with 0.50 mg L− 1 Zeatin formed of 101 ± 0.37 PLBs in the 7th week of culture under red LED light. Furthermore, this is the first report of PLBs induction in P. fragans using Zeatin, as a plant growth regulator, from the use of protocorms.
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Author Information
Instituto de Biotecnología y Ecología Aplicada (INBIOTECA), Universidad Veracruzana, Xalapa, México