*Article not assigned to an issue yet
Baishya Navanita, Kalita Prakash, Pegu Lolesh, Saikia Amarjit
Keywords: Black gram, Delayed sowing, GA3n , Salicylic acid, Physio-biochemical traits, Yield
An experiment was carried out to examine the effects of foliar applied gibberellic acid (GA3) and salicylic acid (SA) in improving the productivity of black gram under delayed sowing condition in Assam, India. Five black gram genotypes viz. LBG-752, PU-30, PU-40, AZAD URD-3 and LBG-645 were sown in three different dates viz. 15th September (D1), 1st October (D2), and 15th October (D3) and were foliar applied with three treatments viz. water spray (T1), 50 ppm GA3 (T2) and 500 µML− 1 salicylic acid (T3) before flowering stage. Significant variations were recorded due to the genotypes, treatments as well as sowing dates in the morpho-physiological, biochemical and yield attributing parameters studied. The plant height, leaf area per plant, whole plant biomass, total leaf chlorophyll content, nitrate reductase enzyme activity, number of pods and seed yield per plant exhibited a declining trend under delayed dates of sowing compared to that of sowing of seeds on 15th of September (D1). However, the proline content and lipid peroxidation were found to be increased under delayed dates of sowing in all the genotypes. The sowing of seeds on 15th of September was found to be the ideal sowing date for black gram in Assam (India) under delayed sowing condition. Furthermore, the GA3 treated plants showed the best performance for most of the traits as compared to salicylic acid and control (water spray) under all the sowing dates and in all the genotypes. The genotype LBG-645 showed the best performance followed by AZAD URD-3 under delayed sowing dates.
Abu-Muriefah SS, Ibrahim MM, Elgaaly GA (2014) Biochemical and photosynthetic evaluation of responses in Zea mays L. under drought stress. Curr World Environ 9(1):87–95
Ahmad A, Aslam Z, Naz M, Hussain S, Javed T, Aslam S, Raza A, Ali HM, Siddiqui MH, Salem MZM, Hano C, Shabbir R, Ahmar S, Saeed T (2021) Exogenous salicylic acid-induced drought stress tolerance in wheat (Triticum aestivum L.) grown under hydroponic culture. PLoS ONE 16(12):e0260556. https://doi.org/10.1371/journal.pone.0260556
Akbari N, Barani M, Ahmadi H (2008) Effect of GA3 on agronomic traits of green gram irrigated with different levels of saline water. World Appl Sci J 5(2):199–203
Alam MM, Nahar K, Hasanuzzaman M, Fujita M (2014) Exogenous jasmonic acid modulates the physiology, antioxidant defense and glyoxalase systems in imparting drought stress tolerance in different Brassica species. Plant Biotechnol Rep 8:279–293
Anitha Y, Vanaja M, Sunitha V, Sowmya P, Sathish P, Kumar GV (2015) Performance of black gram genotypes under moisture deficit stress-variability in physiological and yield contributing attributes. Int J Curr Sci 17(E):82–89
Aydin B, Nalbantoglu B (2011) Effects of cold and salicylic acid treatments on nitrate reductase activity in spinach leaves. Turkish J Biology 35(4):443–448
Barjas M, Mehravaran L, Allahdou M, Ganjali S (2022) Effects of application of salicylic acid under the drought stress on morpho-physiological and biochemical traits of two varieties of mung bean (Vigna radiata L.) in Sistan. J Crop Sci Res Arid Reg 4(1):153–171
Baroowa B, Gogoi N (2014) Biochemical changes in black gram and green gram genotypes after imposition of drought stress. J Food Legumes 27(4):350–353
Baroowa B, Gogoi N (2016) Morphophysiological and yield responses of black gram (Vigna mungo L.) and green gram (Vigna radiate L.) genotypes under drought at growth stages. Res J Recent Sci 5(2):43–50
Bates LS, Waldren RP, Tear ID (1973) Rapid determination of free proline in water stress studies. Plant Soil 39:205–207
Dixit GP, Srivastava AK, Ali H (2024) Scenario of pulses production in India. Indian Farming 74(2):03–06
Elahi NN, Raza S, Rizwan MS, Albalawi BFA, Ishaq MZ, Ahmed HM, Mehmood S, Imtiaz M, Farooq U, Rashid M, Ditta A (2023) Foliar application of gibberellin alleviates adverse impacts of drought stress and improves growth, physiological and biochemical attributes of canola (Brassica napus L). Sustainability 15:1. https://doi.org/10.3390/su15010078
Elangaimannan R, Anbuselvam Y, Karthikeyan P (2008) Genetic diversity in black gram [Vigna mungo (L.) Hepper]. Legume Res 31(1):57–59
Ferrario-Mery S, Valadier MH, Foyer CH (1998) Overexpression of nitrate reductase in tobacco delays drought-induced decrease in nitrate reductase activity and mRNA. Plant Physiol 117(1):293–302
Furlan AL, Bianucci E, Giordano W, Castro S, Becker DF (2020) Proline metabolic dynamics and implications in drought tolerance of peanut plants. Plant Physiol Biochem 151:566–578
Goldani M, Dolatkhahi A, Parsa M, Vahdati N, Rasouli Z (2021) Investigation of improving the drought tolerance in Persian petunia (Petunia sp.) by exogenous application of salicylic acid and gibberellic acid. Acta Scientiarum Polonorum Hortorum Cultus 20(1):37–48
Guo T, Gull S, Ali MM, Yousef AF, Ercisli S, Kalaji HM, Telesiński A, Auriga A, Wróbel J, Radwan NS, Ghareeb RY (2022) Heat stress mitigation in tomato (Solanum lycopersicum L.) through foliar application of gibberellic acid. Sci Rep 12:11324. https://doi.org/10.1038/s41598-022-15590-z
Habib S, Tariq H, Ashfaq S, Nasir N, Majid SA, Ishaq S, Fareed K (2021) Phytohormones ameliorated the physiological changes during post germination drought in maize. J Biology Agric Healthc 11(15):1–14
Hasan BS, Rasul SA (2022) Foliar application effect of salicylic acid and drought stress on growth and yield of mung bean (Vigna radiata). ZANCO J Pure Appl Sci 34(5):103–113
Hayat Q, Hayat S, Irfan M, Ahmad A (2010) Effect of exogenous salicylic acid under changing environment: a review. Environ Exp Bot 68(1):14–25
Heath LR, Packer L (1968) Photo-oxidation in isolated chloroplast. I kinetics and stochiometry of fatty acid peroxidation. Arch Biochem Biophys 125(1):189–198
Hiscox JD, Israelstam GF (1979) A method for the extraction of chlorophyll from leaf tissue without maceration. Can J Bot 57(12):1332–1334
Hussain S, Saleem MF, Ali A, Iqbal J, Shakir MA (2013) Yield and quality improvement of sunflower hybrid through ABA application under water deficit conditions. J Anim Plant Sci 23(4):1158–1165
Jadhav S, Chand S, Patted P, Vishwanath K (2019) Influence of plant growth regulators and micronutrients on seed quality of black gram (Vigna mungo L.) cv. LBG-625 (Rashmi). Int J Pure Appl Bioscience 7(3):115–121
Kaur S, Sharma P (2015) Correlation between leaf traits and moisture availability in Indian mustard (Brassica juncea L). Appl Biol Res 17(2):174–184
Kaya C, Tuna AL, Alves A (2006) Gibberellic acid improves water deficit tolerance in maize plants. Acta Physiol Plant 28(4):331–337
Khan SU, Gurmani AR, Jalal-Ud-Din, Qayyum A, Abbasi KS, Liaquat M, Ahmad Z (2016) Exogenously applied gibberellic acid, indole acetic acid and kinetin as potential regulators of source-sink relationship, physiological and yield attributes in rice (Oryza sativa) genotypes under water deficit conditions. Int J Agric Biology 18(1):139–145
Khan W, Prithiviraj B, Smith D (2003) Photosynthetic responses of corn and soybean to foliar application of salicylates. J Plant Physiol 160(5):485–492
Klepper L, Flesher D, Hageman RH (1971) Generation of reduced nicotinamide adenine dinucleotide for nitrate reduction in green leaves. Plant Physiol 48(5):580–590
Liu J, Wang YS (2020) Proline metabolism and molecular cloning of AmP5CS in the mangrove Avicennia marina under heat stress. Ecotoxicology 29:698–706
Maggio A, Barbieri G, Raimondi G, De PS (2010) Contrasting effects of GA3 treatments on tomato plants exposed to increasing salinity. J Plant Growth Regul 29(1):63–72
Moumita, Mahmud JA, Biswas PK, Nahar K, Fujita M, Hasanuzzaman M (2019) Exogenous application of gibberellic acid mitigates drought-induced damage in spring wheat. Acta Agrobotanica 72(2):1–18
Moumita, Mahmud JA, Biswas PK, Nahar K, Fujita M, Hasanuzzaman M (2019b) Exogenous application of gibberellic acid mitigates drought-induced damage in spring wheat. Acta Agrobotanica 72(2):1776. https://doi.org/10.5586/aa.1776
Mujahid M, Akram MZ, Nazeer S (2022) Evaluation of mung bean (Vigna radiata) under drought stress by foliar application of salicylic acid. J Agric Food Environ Anim Sci 3(1):1–14
Nagasubramaniam A, Pathmanabhan G, Mallika V (2007) Studies on improving production potential of baby corn with foliar spray of plant growth regulators. Annu Rev Plant Physiol Plant Mol Biol 21:154–157
Naresh RK, Purushottam S, Singh P, Dwivedi A, Kumar V (2013) Effects of water stress on physiological processes and yield attributes of different mung bean (Vigna radiate L.) varieties. Afr J Biochem Res 7(5):55–62
Nilanthi D, Ranawake AL, Senadhipathy DD (2014) Effects of water stress on the growth and reproduction of black gram (Vigna mungo L). Trop Agricultural Res Ext 17(1):45–48
Noor F, Hossain F, Ara U (2017) Effects of gibberellic acid (GA3) on growth and yield parameters of French bean (Phaseolus vulgaris L). J Asiatic Soc Bangladesh Sci 43(1):49–60
Ohashi Y, Nakayama N, Saneoka H, Fujita K (2006) Effects of drought stress on photosynthetic gas exchange, chlorophyll fluorescence and stem diameter of soybean plants. Biol Plant 50(1):138–141
Pan S, Rasul F, Li W, Tian H, Mo Z, Duan M, Tang X (2013) Roles of plant growth regulators on yield, grain qualities and antioxidant enzyme activities in super hybrid rice (Oryza sativa L). Rice 6:9. https://doi.org/10.1186/1939-8433-6-9
Pandey HC, Baig MJ, Bhatt RK (2012) Effect of moisture stress on chlorophyll accumulation and nitrate reductase activity at vegetative and flowering stage in Avena species. Agricultural Sci Res J 2(3):111–118
Rahman MM, Khan ABMMM, Hasan MM, Banu LA, Howlader MHK (2018) Effect of foliar application of gibberellic acid on different growth contributing characters of mung bean. Progressive Agric 29(3):233–238
Shan L, Xu Y, Wu D, Hu J, Yu T, Dang C, Fang Y, Zhang X, Tian Q, Xue D (2024) Effects of salicylic acid on growth, physiology, and gene expression in rice seedlings under salt and drought stress. Plant Stress 11:100413. https://doi.org/10.1016/j.stress.2024.100413
Sharma P (2015) Growth dynamics and leaf characteristics of pulse under water deficit and irrigation regimes. Annals Plant Soil Res 17(2):150–155
Singha DL, Sarma S, Singh S (2020) Understanding the mode of regulation of proline biosynthesis for drought tolerance in transgenic rice overexpressing PDH47 gene. Indian J Biotechnol 19:73–81
Sujatha KB (2001) Effect of foliar spray of chemicals and bioregulators on growth and yield of green gram (Vigna radiata L.). M.Sc. (Agri.) Thesis, Tamil Nadu Agric. Univ., Coimbatore
Urmi TA, Islam MM, Zumur KN, Abedin MA, Haque MM, Siddiqui MH, Murata Y, Hoque MA (2023) Combined effect of salicylic acid and proline mitigates drought stress in rice (Oryza sativa L.) through the modulation of physiological attributes and antioxidant enzymes. Antioxidants 12(7):1438. https://doi.org/10.3390/antiox12071438
Zhang H, Pei Y, He Q, Zhu W, Jahangir M, Haq SU, Khan A, Chen R (2023) Salicylic acid-related ribosomal protein CaSLP improves drought and Pst.DC3000 tolerance in pepper. Mol Hortic 3(1):6. https://doi.org/10.1186/s43897-023-00054-3
Department of Crop Physiology, Assam Agricultural University, Assam, India