Effect of EGTA and Trifluoperazine dihydrochloride (TFP) on conidial germination of Marssonina coronaria

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Print ISSN : 0970-4078.
Online ISSN : 2229-4473.
Website:www.vegetosindia.org
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Doi: 10.1007/s42535-023-00800-4
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Keywords: n Diplocarpon malin , Anti-calmodulin, Apple leaf blotch, CaM-signaling


Abstract

We present evidence that treatment of Marssonina coronaria conidia with different concentrations of EGTA and anti-calmodulin drug Trifluoperazine dihydrochloride (TFP) in PPDB resulted in inhibition of spore germination and colony formation. Our study will provide a base to understand the basis of CaM-mediated signaling mechanism in M. coronaria and apple leaf blotch disease development.


n Diplocarpon malin , Anti-calmodulin, Apple leaf blotch, CaM-signaling


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References

Berridge MJ, Bootman MD, Roderick HL (2003) Calcium signalling: dynamics, homeostasis and remodelling. Nat Rev Mol Cell Biol 4(7):517–529. https://doi.org/10.1038/nrm1155. PMID: 12838335


Berridge MJ (2004) Calcium signal transduction and cellular control mechanisms. Biochim BiophysActa. https://doi.org/10.1016/j.bbamcr.2004.08.012


Chauhan A, Sharma JN, Modgil M, Siddappa S (2018) Comparison of various RNA extraction methods, cDNA preparation and isolation of calmodulin gene from a highly melanized isolate of apple leaf blotch fungus Marssoninacoronaria. J Microbiol Methods 151:7–15


Chin D, Means AR (2000) Calmodulin: a prototypical calcium sensor. Trends Cell Biol. https://doi.org/10.1016/s0962-8924(00)01800-6


Hudmon A, Schulman H (2002) Structure-function of the multifunctional Ca2+/calmodulin-dependent protein kinase II. Biochem J. https://doi.org/10.1042/BJ20020228


Kim MH, Choi YJ, Kwon B, Choo YM, Yu KY, Kim J (2019) Regulation of secondary metabolism by calmodulin signaling in filamentous fungi. Rev IberoamMicol, https://doi.org/10.1016/j.riam.2019.04.007. Epub 2019 Sep 14. PMID: 31530465


Laxmi V, Tamuli R (2015) The Neurosporacrassacmd, trm-9, and nca-2 genes play a role in growth, Development, and survival in stress conditions. Genomics and Applied Biology. https://doi.org/10.5376/gab.2015.06.0007


Rodriguez-Caban J, Gonzalez-Velazquez W, Perez-Sanchez L, Gonzalez-Mendez R, Rodriguez-del Valle N (2011) Calcium/calmodulin kinase1 and its relation to thermotolerance and HSP90 in Sporothrixschenckii: an RNAi and yeast two-hybrid study. BMC Microbiol. https://doi.org/10.1186/1471-2180-11-162


Roy A, Kumar A, BaruahD, TamuliR (2020) calcium signaling is involved in diverse cellular processes in fungi. Mycology. https://doi.org/10.1080/21501203.2020.1785962


Sadakane Y, Nakashima H (1996) Light-induced phase shifting of the circadian conidiation rhythm is inhibited by calmodulin antagonists in Neurosporacrassa. J Biol Rhythm 11:234–240


Suresh K, Subramanyam C (1997) A putative role for calmodulin in the activation of Neurosporacrassa chitin synthase. FEMS Microbiol Lett 150:95–100


Suzuki S, Katagiri S, Nakashima H (1996) Mutants with altered sensitivity to a calmodulin antagonist affect the circadian clock in Neurosporacrassa. Genetics 143:1175–1180




 


Acknowledgements


Author Information

Chauhan Arjun
Department of Biotechnology, College of Horticulture, Dr Y S Parmar University of Horticulture and Forestry, Solan, India