Distribution of endophytic fungi associated with Meriandra bengalensis Benth. and assessment of their bioactive potential in vitro

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Research Articles | Published:

Print ISSN : 0970-4078.
Online ISSN : 2229-4473.
Website:www.vegetosindia.org
Pub Email: contact@vegetosindia.org
Doi: 10.1007/s42535-022-00374-7
First Page: 995
Last Page: 1006
Views: 599


Keywords: Fungal endophytes, Seasonal variation, Biocontrol, GC–MS analysis, Antibacterial activity


Abstract


Fungal endophytes are known to be diverse and establish a mutualistic relationship with the host plant and protect them from the soil borne pathogens. In this study, we isolated a total of 283 fungal strains belonging to 8 different genera and 16 morphotypes from 450 tissue segments (Leaf, stems and flowers) of bengle sage (Meriandra bengalensis Benth.) plants during different (winter, summer and rainy) seasons, grown under natural conditions of Manipur and identified them on the basis of cultural morphology. The percentage of isolation rate (IR %) and colonization rates (CR %) of endophtic fungi communities were high in leaf segments (51% and 66%) and winter season (47% and 75%), respectively. The highest relative abundance (RA %) and isolation frequency (IF %) were recorded with Colletotrichum sp.2 and diversity indices of fungal endophytes were also calculated. The bioactive potential of Phoma sp. MBS13 was evaluated in vitro by dual culture tests against Aspergillus flavus, Aspergillus niger, Alternaria solani and Fusarium oxysporum isolates, which showed strong (Class 1and Class 2) antagonistic activity. In addition, the spectral (FTIR and GC–MS) analysis of ethyl acetate extracted metabolites of Phoma sp. MBS13 revealed the presence of various volatile compounds, and showed a good inhibitory activity against some human pathogenic bacteria i.e. Bacillus subtilis, Staphylococcus aureus, Escherichia coli, and Salmonella typhi. Maximum inhibitory zone (15.3 ± 0.15 mm) was recorded with E. coli. Thus, the present findings reveal the importance of endophytic fungi for their role in antimicrobial activity and biocontrol potential against pathogenic microorganisms that could be exploited by the biotechnological and agricultural industries.


Fungal endophytes, Seasonal variation, Biocontrol, GC–MS analysis, Antibacterial activity


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Acknowledgements


The first author gratefully acknowledges the Head of the Department of Life Sciences for providing lab facilities, to carry out this research work.


Author Information


Devi Wairokpam Sanahal
Department of Life Sciences, Manipur University, Imphal, India

Surendirakumar Kannaiah
Department of Life Sciences, Manipur University, Imphal, India
surenderpbt@gmail.com