Vegetos
(An International Journal of Plant Research & Biotechnology)
Comparative analysis of different DNA extraction protocols for optimizing DNA isolation in Eleusine coracana L.
Gupta Shalani*, Garg Rashmi, Sirohi Anil
Research Articles | Published: 09 July, 2013
Online ISSN : 2229-4473.
Website:www.vegetosindia.org
Pub Email: contact@vegetosindia.org
First Page: 262
Last Page: 265
Views: 1181
Keywords:
Finger millet, DNA isolation, PCR, ISSR markers, SDS, CTAB, Sarkosyl.
Abstract
The protocols evaluated included the CTAB method, Sodium Dodecyl Sulfate (SDS Method) and Sarkosyl Method without using liquid nitrogen. All three protocols evaluated effectively to isolate the DNA from each of the four genotypes of finger millet. The quality and quantity of the DNA extracted was compared through agarose gel electrophoresis as well as using UVspectrophotometer. It was found that CTAB and SDS both the methods are giving results at par with superiority of SDS over CTAB in the amplification of PCR products.
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References
Acknowledgements
Author Information
Department of Molecular Biology & Genetic Engineering, College of Biotechnology, Sardar Vallabhbhai Patel University of Agriculture & Technology, Meerut-250 110, India
*Corresponding author Email: sgupta_mbge@yahoo.com