Characterization and antibacterial activity of endophytic fungi isolated from Avicennia marina

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Research Articles | Published:

Print ISSN : 0970-4078.
Online ISSN : 2229-4473.
Website:www.vegetosindia.org
Pub Email: contact@vegetosindia.org
Doi: 10.1007/s42535-023-00582-9
First Page: 163
Last Page: 172
Views: 359


Keywords: Antibacterial, Avicennia marina , Bioprospecting, Endophytic fungi, UPGMA


Abstract


In the present study, we investigated the antibacterial activity of potent endophytic fungi isolated from the roots of Avicennia marina, a mangrove species collected from the Gulf of Kutch, Gujarat, India (23°01′58.6"N 70°09′27.3"E). About thirteen fungal endophytes were isolated from the roots of A. marina utilizing Czapek dox agar medium (10% w/v, NaCl and pH, 7.2). Antibacterial activity against selected pathogenic bacterial test cultures was checked by agar well diffusion method using ethyl acetate extracts of 3 weeks old fungal cultures. Very strong antibacterial activity was recorded against a diverse range of bacterial pathogens, including Shigella flexneri, Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, Bacillus cereus and Klebsiella pneumoniae. Six fungal isolates, which displayed the antibacterial activities, were subjected to the molecular identification. The rRNA internal transcribed spacer (ITS) sequences with high similarity indexes were obtained from GenBank. The phylogenetic analysis of these fungal isolates was performed using the maximum likelihood method, which showed that the selected six fungal isolates belonged to Alternaria, Aspergillus and Penicillium. Three fungal extracts of isolate TR-1 10(7), KR-1 10(14) and TR-2 10(32) displayed broad-spectrum antibacterial activity. Among the three isolates, the crude extract of Penicillium sp. TR-1 10(7) showed broad-spectrum activity against all the test cultures used in the study. The findings suggest that the isolated endophytic fungi from A. marina could be a reservoir of unusual bioactive metabolites. This is the very first report on the antibacterial potential of endophytic fungi, derived from Avicennia marina- belonging to the Gulf of Kutch (Western India) to the best of our knowledge.


Antibacterial, 
                Avicennia marina
              , Bioprospecting, Endophytic fungi, UPGMA


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References


Abeysinghe PD (2010) Antibacterial activity of some medicinal mangroves against antibiotic resistant pathogenic bacteria. Indian J Pharm Sci 72(2):167–172


Aly AH, Debbab A, Proksch P (2011) Fungal endophytes: unique plant inhabitants with great promises. Appl Microbiol Biotechnol 90:1829–1845


Amann RI, Ludwig W, Schleifer KH (1995) Phylogenetic identification and in situ detection of individual microbial cells without cultivation. Microbiol Rev 59(1):143–169. https://doi.org/10.1128/mr.59.1.143-169.1995


Amin N (2016) Endophytic fungi to control of cocoa pod borer (Conopomorpha cramerella) on Cocoa plantation. Res J Pharm Biol Chem Sci 7(6):1496–1501


Bauer AW, Kirby WMM, Sherris John C, Turck M (1966) Antibiotic susceptibility testing by a standardized single disk method. Am J Clin Pathol 45:493–496


Bernardi-Wenzel J, Garcia A, Rubim Filho CJ, Prioli AJ, Pamphile JA (2010) Evaluation of foliar fungal endophyte diversity and colonization of medicinal plant Luehea divaricata (Martius et Zuccarini). Bio Res 43(4):375–384


Bhatt S, Shah DG, Desai N (2009) The mangrove diversity of Purna Estuary, South Gujarat. India Trop Ecol 50(2):287–293


Cao J, Li XM, Li X, Li HL, Meng LH, Wang BG (2019) New lactone and isocoumarin derivatives from the marine mangrove-derived endophytic fungus Penicillium coffeae MA-314. Phytochem Lett 32:1–5


Chen Y, Liu Z, Liu H, Pan Y, Li J, Liu L, She Z (2018) Dichloroisocoumarins with potential anti-inflammatory activity from the mangrove endophytic fungus Ascomycota Sp. CYSK-4. Mar Drugs 16(2):54


Chen Y, Yang W, Zou G, Chen S, Pang J, She Z (2019) Bioactive polyketides from the mangrove endophytic fungi Phoma sp. SYSU-SK-7. Fitoterapia 139:104369


Crozier JAYNE, Thomas SE, Aime MC, Evans HC, Holmes KA (2006) Molecular characterization of fungal endophytic morphospecies isolated from stems and pods of Theobroma cacao. Plant Pathol 55(6):783–791


Cui J, Guo T, Ren Z, Zhang N, Wang M (2015) Diversity and antioxidant activity of culturable endophytic fungi from alpine plants of Rhodiola crenulata, R. angusta, and R. sachalinensis. PLoS ONE 10(3):e0118204


Damião-Cruz C (2008) Programa Genes: Aplicativ ocomputacional emgenéticaestatística. Versão para Windows, Viçosa, Editora UFV


De Souza SFL, Romao-Dumaresq AS, Lacava PT, Harakava R, Azevedo JL, de Melo IS, Pizzirani-Kleiner AA (2013) Species diversity of culturable endophytic fungi from Brazilian mangrove forests. Curr Genet 59(3):153–166


Debbab A, Aly AH, Proksch P (2011) Bioactive secondary metabolites from endophytes and associated marine derived fungi. Fungal Divers 49(1):1–12


Douanla-Meli C, Langer E, Mouafo FT (2013) Fungal endophyte diversity and community patterns in healthy and yellowing leaves of Citrus limon. Fungal Ecol 6:212–222


Elissawy AM, Ebada SS, Ashour ML, El-Neketi M, Ebrahim W, Singab ANB (2019) New secondary metabolites from the mangrove-derived fungus Aspergillus sp. AV-2. Phytochem Lett 29:1–5


Fox EM, Howlett BJ (2008) Biosynthetic gene clusters for epipolythiodioxopiperazines in filamentous fungi. Mycol Res 112:162–169


Giri C, Ochieng E, Tieszen LL, Zhu Z, Singh A, Loveland T, Masek J, Duke N (2011) Status and distribution of mangrove forests of the world using earth observation satellite data. Glob Ecol Biogeogr 20(1):154–159


Hammer Ø, Harper DAT, Ryan PD (2001) PAST: paleontological statistics software package for education and data analysis. Palaeontol Electronica 4(1):9


Handayani D, Rivai H, Hutabarat M, Rasyid R (2017) Antibacterial activity of endophytic fungi isolated from mangrove plant Sonneratia griffithii Kurz. J Appl Pharm Sci 7:209–212. https://doi.org/10.7324/japs.2017.70431


Hartley SE, Eschen R, Horwood JM, Gange AC, Hill EM (2015) Infection by a foliar endophyte elicits novel arabidopside-based reactions in its host. Cirsium Arvense New Phytol 205:816–827


He X, Han G, Lin Y, Tian X, Xiang C, Tian Q, Wang F, He Z (2012) Diversity and decomposition potential of endophytes in leaves of a Cinnamomum camphora plantation in China. Ecol Res 27(2):273–284


Huang H, Liu T, Wu X, Guo J, Lan X, Zhu Q, Zheng X, Zhang K (2017) A new antibacterial chromone derivative from mangrove-derived fungus Penicillium aculeatum (No. 9EB). Nat Prod Res 31(22):2593–2598


Kathiresan K (2010) Importance of mangrove forests of India. J Coast Environ 1(1):70–89


Kjer J, Debbab A, Aly AH, Proksch P (2010) Methods for isolation of marine-derived endophytic fungi and their bioactive secondary products. Nat Protoc 5(3):479–490


Koukol O, Kolarík M, Kolárová Z, Baldrian P (2012) Diversity of foliar endophytes in wind-fallen Picea abies trees. Fungal Divers 54(1):69–77


Kusari S, Pandey SP, Spiteller M (2013) Untapped mutualistic paradigms linking host plant and endophytic fungal production of similar bioactive secondary metabolites. Phytochem 91:81–87


Li H, Qing C, Zhang Y, Zhao Z (2005) Screening for endophytic fungi with antitumour and antifungal activities from Chinese medicinal plants. World J Microbiol Biotechnol 21(8):1515–1519


Nair DN, Padmavathy S (2014) Impact of Endophytic Microorganisms on Plants. The Scientific World Journal, Environment and Humans. https://doi.org/10.1155/2014/2506934


Nurunnabi TR, Sabrin F, Sharif DI, Nahar L, Sohrab MH, Sarker SD, Rahman SM, Billah M (2020) Antimicrobial activity of endophytic fungi isolated from the mangrove plant Sonneratia apetala (Buch.-Ham) from the Sundarbans mangrove forest. Advan Tradition Med. 20(3):419–425. https://doi.org/10.1007/s13596-019-00422-9


Okla MK, Alatar AA, Al-Amri SS, Soufan WH, Ahmad A, Abdel-Maksoud MA (2021) Antibacterial and antifungal activity of the extracts of different parts of Avicennia marina (Forssk.) Vierh. Plants 10(2):252


Pereira S, Zille A, Micheletti E, Moradas-Ferreira P, De Philippis R, Tamagnini PP (2009) Complexity of cyanobacterial exopolysaccharides: composition, structures, inducing factors and putative genes involved in their biosynthesis and assembly. FEMS Microbiol Rev 33(5):917–941


Phongpaichit S, Rungjindamai N, Rukachaisirikul V, Sakayaroj J (2006) Antimicrobial activity in cultures of endophytic fungi isolated from Garcinia species. FEMS Immun Med Microbiol 48(3):367–372


Potshangbam M, Devi SI, Sahoo D, Strobel GA (2017) Functional characterization of endophytic fungal community associated with Oryza sativa L. and Zea mays L Front. Microbiol 8:325


Deng Q, Li G, Sun M, Yang X, Jing Xu (2020) A new antimicrobial sesquiterpene isolated from endophytic fungus Cytospora sp. from the Chinese mangrove plant Ceriops tagal. Nat Prod Res 34(10):1404–1408. https://doi.org/10.1080/14786419.2018.1512993


Rhoden SA, Garcia A, Bongiorno VA, Azevedo JL, Pamphile JA (2012) Antimicrobial activity of crude extracts of endophytic fungi isolated from medicinal plant Trichilia elegans A. Juss J App Pharm Sci 2(8):57


Roome T, Dar A, Ali S, Naqvi S, Choudhary MT (2008) A study on antioxidant, free radical scavenging, anti-inflammatory and hepatoprotective actions of Aegiceras corniculatum (stem) extracts. J Ethnopharmacol 118(3):514–521


Shan TJ, Feng H, Xie Y, Shao C, Wang J, Mao ZL (2019) Endophytic fungi isolated from Eucalyptus citriodora Hook. f. and antibacterial activity of crude extracts. Plant Prot 45:149–155


Spalding MD, Blasco F, Field CD (1997) World mangrove atlas. International Society for Mangrove Ecosystems, Okinawa, Japan


Suiton DC, Gillan FT, Susic M (1985) Naphthofuranone phytoalexins from the grey mangrove. Avicennia Marina Phytochem 24(12):2877–2879


Ukwatta KM, Lawrence JL, Wijayarathne CD (2019) Antimicrobial, anti-cancer, anti-filarial and anti-inflammatory activities of cowabenzophenone a extracted from the endophytic fungus Aspergillus Terreus isolated from a mangrove plant bruguiera gymnorrhyza. Mycology 11(4):297–305


Bandaranayake WM (2002) Bioactivities, bioactive compounds and chemical constituents of mangrove plants. Wetl Ecol Manag 10(6):421–452


Zhang W, Xu L, Yang L, Huang Y, Li S, Shen Y (2014) Phomopsidone A, a novel depsidone metabolite from the mangrove endophytic fungus Phomopsis sp. A123. Fitoterapia 96:146–151


Zhu X, Zhou D, Liang F, Wu Z, She Z, Li C (2017) Penochalasin K, a new unusual chaetoglobosin from the mangrove endophytic fungus Penicillium chrysogenum V11 and its effective semi-synthesis. Fitoterapia 123:23–28

 


Acknowledgements


The authors are grateful to Shri M.M Patel Institute of Sciences and Research, Gandhinagar, Gujarat, India for providing laboratory facilities.


Author Information


Trivedi Nidhi S.
Department of Microbiology and Biotechnology, School of Sciences, Gujarat University, Ahmedabad, India

Thumar Jignasha T.
Department of Microbiology, Government Science College, Gandhinagar, India
jignashathumar@gmail.com