Assessment of genetic diversity among species mungo-radiata group of subgenus Ceratotropis of genus Vigna Savi. using amplified fragment length polymorphism (AFLP)

*Article not assigned to an issue yet

, , ,


Research Articles | Published:

Print ISSN : 0970-4078.
Online ISSN : 2229-4473.
Website:www.vegetosindia.org
Pub Email: contact@vegetosindia.org
Doi: 10.1007/s42535-023-00683-5
First Page: 0
Last Page: 0
Views: 1000


Keywords: AFLP, Asiatic Vignan , Ceratotropis, Population substructure, Gene flow


Abstract


Genetic diversity, population substructure, species relationships, and gene flow was evaluated in 85 accessions belonging to the mungo-radiata group of section Ceratotropis of subgenus Ceratotropis of genus Vigna using amplified fragment length polymorphism. Twelve preselected AFLP primers generated 1869 polymorphic amplification products. The number of fragments for each primer pair ranged from 44 to 296, showing 100% polymorphism. An analysis of the mungo-radiata group from different phytogeographical regions across India revealed a high level of genetic diversity. The six species of the mungo-radiata group were assembled into three groups namely, Radiata, Mungo, and Hainiana. The highest total diversity as well as within accession diversity was obtained for the Hainiana group and minimum for the Mungo group. Genetic differentiation values for Mungo and Radiata group was 0.158 and 0.212, respectively. A high gene flow was detected within each group. For mungo-radiata-hainiana relatives, Fsc, Fct, and Fst estimates were 0.120, 0.089, and 0.034, respectively, and all Vigna species combined, the fixation indices Fst, Fsc and Fct were 0.120, 0.068, and 0.034, respectively. A tree with a star-shaped topology was obtained, in which the accessions from all the species are intermingled further substantiates gene flow between populations.


AFLP, Asiatic Vignan                  , Ceratotropis, Population substructure, Gene flow


*Get Access

(*Only SPR Members can get full access. Click Here to Apply and get access)

Advertisement

References


Arens P, Coops H, Jansen J, Vosman B (1998) Molecular genetic analysis of black poplar (Populous nigra) along Dutch rivers. Mol Ecol 7:11–18


Beebe S, Toro Ch, Gonzalez O, Chacon MI, Debouck DG (1997) Wild-weed-crop groups of common bean (Phaseolus vulgaris L., Fabaceae) in the Andes of Peru and Columbia, and their implications for conservation and breeding. Genet Res Crop Evol 44:73–91


Bhat KV, Lakhanpaul S, Chadha S (2005) Amplified fragment length polymorphism (AFLP) analysis of genetic diversity in Indian mungbean [Vigna radiata (L.) Wilczek] cultivars. Indian J Biotechnol 4:56–64


Bhattacharyya P, Ghosh S, Mandi SS et al (2017) Genetic variability and association of AFLP markers with some important biochemical traits in Dendrobium thyrsiflorum, a threatened medicinal orchid. S Afr J Bot 109:214–222


Brunk CF, Jones KC, James TW (1979) Assay for nanogram quantities of DNA in cellular homogenates. Annal Biochem 92:497–500


Egawa Y, Nakagawara M, Fernandez GCJ, Gatehouse AMR (1990) Cross compatibility and cytogenetical relationships among Asian Vigna species. In: Fuzii K, Johnson CD, Mitchel R, Yoshida T (eds) Bruchids and legumes: economics, ecology and coevolution. Kluwer Academic Publishers, pp 201–208





Excoffier L, Smouse PE, Quattro JM (1992) Analysis of molecular variance inferred from metric distances among DNA haplotypes: applications to human mitochondrial DNA restriction sites. Genetics 131:479–491


Gaiotto FA, Bramucci M, Grattapaglia D (1997) Estimation of outcrossing rate in a breeding population of Eucalyptus urophylla with dominant RAPD and AFLP markers. Theor Appl Genet 95:842–849


Govindraju DR (1988) Relationship between dispersal ability and levels of gene flow in plants. Oikos 52:31–35


Harlan JR (1965) The possible role of weed races in the evolution of cultivated plants. Euphytica 14:173–176


Harlan JR, DeWet JMJ (1965) Some thoughts about weeds. Econ Bot 19:16–24


Jaccard P (1908) Etude comparative de a distribution florale dans portiondes Alpes et des Jura. Bull Soc Vaud Sci Nat 37:547–579


Kaga A, Tomooka N, Egawa Y, Hosaka K, Kamijima O (1996) Species relationships in the subgenus Ceratotropis (genus Vigna) as revealed by RAPD analysis. Euphytica 88:17–24


Karp A, Seberg O, Buiatti (1996) Molecular techniques in the assessment of botanical diversity. Ann Bot 78:143–149


Koopman WJ, Zevenbergen MJ, Van den Berg RG (2001) Species relationships in Lactuca sativa. (Lactuceae, Asteraceae) inferred from AFLP fingerprints. Am J Bot 88(10):1881–1887


Lewontin R (1972) The apportionment of human diversity. Evol Biol 6:381–398





Liersch A, Bocianowski J, Popławska W et al (2019) Creation of gene pools with amplified fragment length polymorphism markers for development of winter oilseed rape (Brassica napus L.) hybrid cultivars. Euphytica 215:22


Majer D, Mithen R, Lewis BG, Vos P, Oliver RP (1996) The use of AFLP fingerprinting for the detection of genetic variation in fungi. Mycol Res 100:1107–1111


McDermott JM, McDonald BA (1993) Gene flow among plant pathosystems. Annu Rev Phytopathol 31:353–373


Misra A, Shassany AK, Shukla AK, Darokar MP, Singh SC, Sundaresan V, Singh J, Bagchi GD, Jain SP, Saikia D, Khanuja SP (2010) AFLP markers for identification of Swertia species (Gentianaceae). Genet Mol Res 9:1535–1544


Mueller UG, Wolfenbarger LL (1999) AFLP genotyping and fingerprinting. TREE 14:389–394


Muluvi GM, Sprent JI, Soranjo N, Provan J, Odee D, Folkard G, McNicol JW, Powell W (1999) Amplified Fragment Length Polymorphism (AFLP) analysis of genetic variation in Moringa oleifera Lam. Mol Ecol 8:463–470


Nei M (1972) Genetic distance between populations. Am Nat 106:283–292


Nei M (1987) Molecular evolutionary genetics. Columbia University Press, New York


Nei M, Li WH (1979) Mathematical model for studying genetic variation in terms of restriction endonucleases. Proc Natl Acad Sci USA 76:5269–5673


Oka HI, Morishima H (1971) The dynamics of plant domestication: cultivation experiments with Oryza perennis and its hybrid with O. sativa. Evolution 25:356–364


Oliveira TG, Pereira AMS, Coppede JS et al (2016) Genetic diversity analysis of Croton antisyphilIiticus Mart. using AFLP molecular markers. Genet Mol Res 15(1):1–8


Paun O, Schönswetter P (2012) Amplified fragment length polymorphism: an invaluable fingerprinting technique for genomic, transcriptomic, and epigenetic studies. Methods Mol Biol 862:75–87


Rohlf FJ (2002). NTSYS-pc Numerical taxonomy and multivariate taxonomy system, version 2.11, Exeter software, Seteauket, New York





Saravanakumar P, Kaga A, Tomooka N, Vaughan DA (2004) AFLP and RAPD analyses of intra-and interspecific variation in some Vigna subgenus Ceratotropis (Leguminosae) species. Aust J Bot 52:417–424


Seehalak W, Tomooka N, Waranyuwar A et al (2006) Genetic diversity of the Vigna germplasm from Thailand and neighboring regions revealed by AFLP analysis. Genet Resour Crop Evol 53:1043–1059


Shannon CE, Weaver W (1949) The mathematical theory of communication. University of Illinois Press, Urbana


Sheeja TE, Kumar IPV, Giridhari A, Minoo D, Rajesh MK, Babu KN (2021) Amplified fragment length polymorphism: applications and recent developments. Methods Mol Biol 2222:187–218. https://doi.org/10.1007/978-1-0716-0997-2_12. (PMID: 33301096)


Slatkin M (1985) Rare alleles as indicators of gene flow. Evolution 39(1):53–65


Smartt J (1980) Evolution and evolutionary problems in food legumes. Econ Bot 34:219–235


Soltis PS, Bloom WL (1986) Genetic variation and estimates of gene flow in Clarkia speciosa subsp. polyantha (Onagraceae). Am J Bot 73(12):1677–1782





Tomooka N, Yoon MS, Doi K, Kaga A, Vaughan D (2002) AFLP analysis of diploid species in the genus Vigna subgenus Ceratotropis. Genet Res Crop Evol. 49:521–530





Tripathi N, Saini N, Tiwari S (2011) Assessment of genetic diversity among Aloe vera accessions using amplified fragment length polymorphism. Int J Med Arom Plants 1:115–121


Tripathi N, Saini N, Nair P, Tiwari S (2012) Lack of genetic diversity of a critically endangered important medicinal plant Chlorophytum borivilianum in central India revealed by AFLP markers. Physiol Mol Biol Plants 18(2):161–167


Varma A, Shrivastava N (2018) Genetic structuring in wild populations of two important medicinal plant species as inferred from AFLP markers. Plant Biosyst 152(5):1088–1100


Vir R, Bhat KV, Lakhanpaul S (2009) Analysis of population substructure, genetic differentiation and phylogenetic relationships among selected Asiatic Vigna species. Genet Res Crop Evol 56:783–795


Vos P, Hogers R, Bleeker R, Reijans M, VandeLee T, HomesM FA, Pot P, Peleman J, Kuiper M, Zabeau M (1995) AFLP: a new technique for DNA fingerprinting. Nuc Acids Res 23:4407–4414


Wanjala BW, Obonyo M, Wachira FN, Muchugi A, Mulaa M, Skilton RA, Proud J, Hanson J (2013) Genetic diversity in Napier grass (Pennisetum purpureum) cultivars: implications for breeding and conservation. AoB Plants. 5:plt022

 


Acknowledgements



Author Information


Vir Ruchi
Department of Botany, Zakir Husain Delhi College, Delhi University, New Delhi, India
ruchivir@zh.du.ac.in
Jehan Tabassum
Department of Botany, Zakir Husain Delhi College, Delhi University, New Delhi, India


Bhat K. V.
National Bureau of Plant Genetic Resources, National Research Centre on DNA Fingerprinting, New Delhi, India


Lakhanpaul S.
Department of Botany, Delhi University, New Delhi, India