Assessment of bacterial diversity and their antibiotic resistance profiles in wastewater treatment plants and their receiving Ganges River in Prayagraj (Allahabad), India

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Research Articles | Published:

E-ISSN: 2229-4473.
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DOI: 10.1007/s42535-020-00157-y
First Page: 744
Last Page: 749
Views: 3647

Keywords: Bacterial diversity, Municipal wastewater, Ganges River, Antibiotics-resistant bacteria, 16S rRNA sequencing


Abstract

The wastewater treatment plant is the silent reservoir of the broad spectrum of microbial populations, including pathogenic ones, which have the potential to cause contamination and pose risks to public and environmental health. In this work, we estimated the actual bacterial load present in the pre and post-treated wastewater samples from WWTPs of Prayagraj region of India. Furthermore, we also studied the distribution of antibiotic-resistant bacteria (ARB) from WWTPs to Ganges river samples. Data indicated that the majority of the isolates were identified as Bacillus spp. (42.4%) followed by Gammaproteobacteria (27%) which included members of the genera Aeromonas, Acinetobacter, Pseudomonas and Enterobacter. Most bacterial isolates regardless of the site of sampling resulted resistance to cefotaxime and ampicillin (50% and 41.6%) respectively. This study concluded that the municipal wastewater offers an atmosphere conducive to bacteria with antibiotic resistance that can harbor resistant bacteria and their resistant genes to receiving water sections with potential public health danger.



References

  1. Aslam B, Wang W, Arshad MI, Khurshid M, Muzammil S, Rasool MH et al (2018) Antibiotic resistance: a rundown of a global crisis. Infect Drug Resist 11:1645–1658

  2. Deng Y, Xu H, Su Y et al (2019) Horizontal gene transfer contributes to virulence and antibiotic resistance of Vibrio harveyi 345 based on complete genome sequence analysis. BMC Genom 20:761

  3. Fiorentino A, Di Cesare A, Eckert EM, Rizzo L, Fontaneto D, Yang Y, Corno G (2019) Impact of industrial wastewater on the dynamics of antibiotic resistance genes in a full-scale urban wastewater treatment plant. Sci Total Environ 646:1204–1210

  4. Gallert C, Fund K, Winter J (2005) Antibiotic resistance of bacteria in raw and biologically treated sewage and in groundwater below leaking sewers. Appl Microbiol Biotechnol 69(1):106–112

  5. Guo J, Li J, Chen H, Bond PL, Yuan Z (2017) Metagenomic analysis reveals wastewater treatment plants as hotspots of antibiotic resistance genes and mobile genetic elements. Water Res 123:468–478

  6. Hultman J, Tamminen M, Pärnänen K, Cairns J, Karkman A, Virta M (2018) Host range of antibiotic resistance genes in wastewater treatment plant influent and effluent. FEMS Microbiol Ecol 94(4):fiy038

  7. Kesari KK, Tripathi P, Tripathi V (2018) Influence of wastewater use in agriculture: advances in human and plant health. Adv Microb Biotechnol 1:232–246

  8. Kummerer K (2004) Resistance in the environment. J Antimicrob Chemother 54(2):311–320

  9. Lalucat J, Bennasar A, Bosch R, García-Valdés E, Palleroni NJ (2006) Biology of Pseudomonas stutzeri. Microbiol Mol Biol Rev 70(2):510–547

  10. Lood R, Ertürk G, Mattiasson B (2017) Revisiting antibiotic resistance spreading in wastewater treatment plants—bacteriophages as a much neglected potential transmission vehicle. Front Microbiol 8:2298

  11. Ma L, Zhang XX, Zhao F, Wu B, Cheng S, Yang L (2013) Sewage treatment plant serves as a hot-spot reservoir of integrons and gene cassettes. J Environ Biol 34:391–399

  12. Manaia CM, Rocha J, Scaccia N, Marano R, Radu E, Biancullo F, Cerqueira F, Fortunato G, Iakovides IC, Zammit I, Kampouris I, Vaz-Moreira I, Nunes OC (2018) Antibiotic resistance in wastewater treatment plants: tackling the black box. Environ Int 115:312–324

  13. Murray PR, Baron EJ, Pfaller MA, Tenover FC, Yolke RH (1995) Manual of clinical microbiology, 6th edn. ASM, Washington, DC

  14. Mutiyar PK, Mittal AK (2014) Occurrences and fate of selected human antibiotics in influents and effluents of sewage treatment plant and effluent-receiving river Yamuna in Delhi (India). Environ Monit Assess 186(1):541–557

  15. Nathan C, Cars O (2014) Antibiotic resistance-problems, progress, and prospects. N Engl J Med 371(19):1761–1763

  16. Nimonkar YS, Yadav B, Talreja P, Sharma A, Patil S, Saware SS, Ranade DR, Prakash O (2019) Assessment of the role of wastewater treatment plant in spread of antibiotic resistance and bacterial pathogens. Indian J Microbiol 59:261–265

  17. Oluseyi CO, Selvarajan R, Mamba BB, Msagati TAM (2019) Profiling bacterial diversity and potential pathogens in wastewater treatment plants using high-throughput sequencing analysis. Microorganisms 7(11):506

  18. Paul D (2017) Research on heavy metal pollution of river Ganga: a review. Ann Agrar Sci 15(2):278–286

  19. Peterson E, Kaur P (2018) Antibiotic resistance mechanisms in bacteria: relationships between resistance determinants of antibiotic producers, environmental bacteria, and clinical pathogens. Front Microbiol 9:2928

  20. Roca I, Akova M, Baquero F et al (2015) The global threat of antimicrobial resistance: science for intervention. New Microbes New Infect 6:22–29

  21. Rossolini GM, Arena F, Pecile P, Pollini S (2014) Update on the antibiotic resistance crisis. Curr Opin Pharmacol 18:56–60

  22. Sib E, Voigt AM, Wilbring G, Schreiber C, Faerber HA, Skutlarek D, Parcina M, Mahn R, Wolf D, Brossart P, Geiser F, Engelhart S, Exner M, Bierbaum G, Schmithausen RM (2019) Antibiotic resistant bacteria and resistance genes in biofilms in clinical wastewater networks, Int J Hyg Environ Health. 222(4):655–662

  23. Sikorski J, Moehle M, Wackernagel W (2002) Identification of complex composition, strong strain diversity and directional selection in local Pseudomonas stutzeri populations from marine sediment and soils. Environ Microbiol 4:465–476

  24. Singer AC, Shaw H, Rhodes V, Hart A (2016) Review of antimicrobial resistance in the environment and its relevance to environmental regulators. Front Microbiol 7:1728

  25. Sun D, Jeannot K, Xiao Y, Knapp CW (2019) Editorial: horizontal gene transfer mediated bacterial antibiotic resistance. Front Microbiol 10:1933

  26. Tripathi V, Cytryn E (2017) Impact of anthropogenic activities on the dissemination of antibiotic resistance across ecological boundaries. Essays Biochem 61(1):11–21 ()

  27. Tripathi V, Tripathi P (2017) Antibiotic resistance genes: an emerging environmental pollutant. Perspect Environ Toxicol 1:183–201

  28. Turolla A, Cattaneo M, Marazzi F, Mezzanotte V, Antonelli M (2018) Antibiotic resistant bacteria in urban sewage: role of full-scale wastewater treatment plants on environmental spreading. Chemosphere 191:761–769

  29. Vadde KK, Feng Q, Wang J, McCarthy AJ, Sekar R (2019) Next-generation sequencing reveals fecal contamination and potentially pathogenic bacteria in a major inflow river of Taihu Lake. Environ Pollut. 254(Pt B):113108

  30. von Wintersdorff CJH, Penders J, van Niekerk JM, Mills ND, Majumder S, van Alphen LB, Savelkoul PHM, Wolffs PFG (2016) Dissemination of antimicrobial resistance in microbial ecosystems through horizontal gene transfer. Front Microbiol 7:173

  31. Wang Q, Wang P, Yang Q (2018) Occurrence and diversity of antibiotic resistance in untreated hospital wastewater. Sci Total Environ 621:990–999

  32. Yao PC, Chen JY, Zhang YM, Wen DH, Chen LJ (2019) Distribution characteristics of antibiotic resistance genes in wastewater treatment plants. Huan Jing Ke Xue 40(11):5024–5031

  33. Yuan QB, Zhai YF, Mao BY, Schwarz C, Hu N (2019) Fates of antibiotic resistance genes in a distributed swine wastewater treatment plant. Water Environ Res 91(12):1565–1575


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Author Information

Department of Molecular and Cellular Engineering, Jacob Institute of Biotechnology and Bioengineering, Sam Higginbottom University of Agriculture, Technology and Sciences, Prayagraj, India