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Nouasri Ahmed, Miri Yamina Ben, Alouache Ali, Benabdalah Amina, Toumi Mohamed
Keywords: Stachys ocymastrum, Phytochemical screening, Cytotoxic and antimicrobial activities
Stachys ocymastrum L., is an aromatic and medicinal plant from the Lamiaceae family. The aerial parts were extracted by maceration with ethanol, then fractionated by petroleum ether, chloroform, and ethyl acetate. The extract and its fractions were subjected to measurement of polyphenol contents, chromatographic analysis, and evaluation of cytotoxicity and antimicrobial activities. Results show its richness in total polyphenols, and its poorness in flavonoids. gallic acid, and quercetin as components identified. The ethanolic extract are the more cytotoxic extract with LC50 value of 30.077 µg/ml. All of the bacterial strains tested were inhibited by our extracts. Ethanol and ethyl acetate were slightly more active than chloroform and petroleum ether. The fungal strains show resistance, The CMI was under 2 mg/ml against Enterobacter cloacae. The present studies showed that the extracts of Stachys ocymastrum have acceptable antimicrobial effects as therapy sources, however, other tests such as antioxidant, and anti-inflammatory can be measured for good valorization of this specie.
Pavan E, Damazo AS, Lemos LMS, Adzu B, Balogun SO, Arunachalam K, Martins DTO (2018) Evaluation of genotoxicity and subchronic toxicity of the standardized leaves infusion extract of Copaifera Malmei harms in experimental models. J Ethnopharmacol 30(211):70–77. https://doi.org/10.1016/j.jep.2017.09.027Epub 2017 Sep 21. PMID: 28943446
Bouasla I, Hamel T, Barour C, Bouasla A, Hachouf M, Bouguerra O, Messarah M (2021) Evaluation of solvent influence on phytochemical content and antioxidant activities of two Algerian endemic taxa: Stachys marrubiifolia Viv. and Lamium flexuosum Ten. (Lamiaceae), Eur. J. Integr. Med. 42:101267. https://doi.org/10.1016/j.eujim.2020.101267
Souza L, Vasconcelos M, Silva A, Silva P, Gouvela Veloso C, Araújo D, Costa V, Pessôa H, Tavares J, Silva M, Silva B, Cavalcante F (2020) Toxicological and Pharmacological activities of Leptohyptis macrostachys (Benth.) Harley and J.F.B. Pastore (Lamiaceae) on intestinal smooth muscle. Front Pharmacol 10:1042. https://doi.org/10.3389/fphar.2020.01042
Bahadori MB, Kirkan B, Sarikurkcu C, Ceylan O (2020a) Metabolite profiling and health benefits of Stachys cretica subsp. Mersinaea as medicinal food. Eur J Integr Med 36:101134. https://doi.org/10.1016/j.indcrop.2019.01.038
Hamel T, Seridi R, De bélair G, Slimani A, Babali B (2013) Flore vasculaire rare et endémique de La péninsule de L’Edough (Nord-Est-Algérien). Rev Sci Technol Synthèse 26:65–74
Quézel P, Santa S (1963) Nouvelle flore de l’Algérie et des régions désertiques méridionales,seconde ed.CNRS, Paris, p. 814
Nouasri A, Krimat S, Dahmane D, Ksouri A, Metidji H, Dob T (2018) Biological activities and chemical analysis of phenolic and flavonoid components of Thymus hirtus Willd. and Thymus lanceolatus Desf. Extracts. Phytotherapie 16(6):353–364. https://doi.org/10.3166/phyto-2018-0020
Takao T, Kitatani F, Watanabe N, Yagi A, Sakata K (1994) A simple screening method for antioxidant and isolation of several antioxidants produced by Marin bacteria from fish and shellfish. Biosc Biotechnol Biochem 58(10):1780–1783. https://doi.org/10.1271/bbb.58.1780
Lawi Y, Saria J, Kidukuli AW (2018) Brine shrimp cytotoxicity, phytochemical screening and larvicidal activities of Plectranthus barbatus extracts. Res Rev Insights 2(1):1–4. https://doi.org/10.13140/RG.2.2.25713.58726
Hamidi М, Jovanova B, Panovska ТK (2014) Toxicоlogical evaluation of the plant products using Brine shrimp (Artemia salina L.) model. Maced Pharm Bull 60(1):9–18. https://doi.org/10.33320/MACED.PHARM.BULL.2014.60.01.002
Bahadori MB, Zengin G, Dinparast L, Eskandanid M (2020b) The health benefits of three Hedgenettle herbal teas (Stachys byzantina.Stachys inflata. and Stachys lavandulifolia) - profiling phenolic and antioxidant activities. Eur J Integr Med 36:101134. https://doi.org/10.1016/j.eujim.2020.101134
Kirkan B (2019) Antioxidant potential, enzyme Inhibition activity, and phenolic profile of extracts from Stachys cretica subsp. Vacillans. Ind Crops Prod 140:111639. https://doi.org/10.1016/j.indcrop.2019.111639
Nakbi H, Dallel W, Hammami S, Mighri Z (2020) Phytochemical profile and antioxidant properties of leaves extractsfrom Posidonia oceanic (L.) delile and their allelopathic potential on terrestrial plant species. Bull Chem Soc Ethiop 34(3):437–447. https://doi.org/10.4314/bcse.v34i3.1
Merah S, Dahmane D, Krimat S, Metidji H, Nouasri A, Lamari L, Dob T (2018) Chemical analysis of phenolic compounds and determination of anti-oxidant. Antimicrobial and cytotoxic activities of organic extracts of Pinus coulteri. Bangladesh J Pharmacol 13(2):120–129
Weli A, Al-Salmi S, Al Hoqani H, Hossain M (2018) Biological and phytochemical studies of different leaves extracts of Pteropyrum scoparium. Beni-Suef Univ Int J Basic Appl 7(4):481–486. https://doi.org/10.1016/j.bjbas.2018.05.001
Jassbi AR, Asadollahi M, Javanmardi N, Firuzi O (2014) Cytotoxic. Antioxidant and antimicrobial effects of nine species of woundwort (Stachys) plants. Pharm Biol 52(1):62–67. https://doi.org/10.3109/13880209.2013.810650
Gulsoy-Toplan G, Taskin T, Mataraci E, Ecevit-Gülay G (2021) Antioxidant and antimicrobial activities of various extracts from Stachys cretica subsp. Bulgarica Rech.f., Stachys byzantina K. Koch and Stachys Thirkei K. Koch. Istanbul J Pharm 51(3):341–347. https://doi.org/10.26650/IstanbulJPharm.2021.974035
Benabderrahim M, Elfalleh W, Ozer S, Ceylan O (2021) Phenolic composition and biological activities of Turkish endemic plant: Stachys cretica subsp. Kutahyensis. S Afr J Bot 138:124–128. https://doi.org/10.1016/j.sajb.2020.12.012
Elfalleh W, Bulent K (2019) Antioxidant potential and phenolic composition of extracts from Stachys tmolea: an endemic plant from Turkey. Ind Crops Prod 127:212–216. https://doi.org/10.1016/j.indcrop.2018.10.078
Lakhal H, Boudiar T, Kabouche A, Laggoune S, Kabouche Z, Topcu G (2011) Antioxidant activity and flavonoids of Stachys ocymastrum. Chem Nat Compd 46:964–965. https://doi.org/10.1007/s10600-011-9797-4
Kocak MS, Uren MC, Calapoglu M, Sihoglu Tepe A, Mocan A, Rengasamy KRR, Sarikurkcu C (2017) Phenolic profile. antioxidant and enzyme inhibitory activities of Stachys annua subsp. annua var. annua. S Afr J Bot 113:128–132.https://doi.org/10.1016/j.sajb.2017.08.005
Bahadoria MB, Kirkanb B, Sarikurkcuc C (2019) Phenolic ingredients And therapeutic potential of Stachys cretica subsp. Smyrnaea for the management of oxidative stress. Alzheimer’s disease. Hyperglycemia. And melasma. Ind Crops Prod 127:82–87. https://doi.org/10.1016/j.indcrop.2018.10.066
Ghaneialvar H, Abbasi N, Saneei S, Zangeneh A, Zangeneh M, Pooyanmehr M, Almasi M, Ghazikhani F, Bahrami E (2021) An ethnomedicinal plant: antibacterial activities of Stachys Lavandulifolia Vahl aqueous extract against common pathogens. Plant Biotechnol Persa 3(1):1–8. https://doi.org/10.52547/pbp.3.1.2
Clarkson C, Maharaj VJ, Crouch NR, Grace OM, Pillay P, Matsabisa MG, Bhagwandin N, Smith PJ, Folb PI (2004) In vitro antiplasmodial activity of medicinal plants native to or naturalised in South Africa. J Ethnopharmacol Jun 92(2–3):177–191. https://doi.org/10.1016/j.jep.2004.02.011
Chanda S, Baravalia Y (2011) Brine shrimp cytotoxicity of Caesalpinia pulcherrima aerial parts, antimicrobial activity and characterisation of isolated active fractions. Nat Prod Res 25(20):1955–1964 https:/. https://doi.org/10.1080/14786419.2010.530600
Kabubii ZN, Mbaria JM, Mbaabuc PM (2015) Phytochemical composition and Brine shrimp cytotoxicity effect of Rosmarinus officinalis. ASRJETS 11(1):127–135. https://core.ac.uk/download/pdf/235049622.pdf
Delnavazi MR, Saiyarsarai P, Jafari-Nodooshan S, Khanavi M, Tavakoli S, Hadavinia H, Yassa N (2018) Cytotoxic flavonoids from the aerial parts of Stachys Lavandulifolia. Vahl Pharm Sci 24(4):332–339 https://doi.org/10.15171/PS.2018.47
Karchesy MY, Kelsey RG, Constantine G, Karchesy JJ (2016) Biological screening of selected Pacific Northwest forest plants using the Brine shrimp toxicity bioassay. Springerplus 23:510. https://doi.org/10.1186/s40064-016-2145-1
Bouzada M, Fabri R, Nogueira M, Konno T, Duarte G, Scio E (2009) Antibacterial, cytotoxic and phytochemical screening of some traditional medicinal plants in Brazil. Pharm Biol 47(1):44–52. https://doi.org/10.1080/13880200802411771
Apu A, Muhit M, Tareq S, PathanA, Jamaluddin A, Ahmed (2020) Antimicrobial activity and Brine shrimp lethality bioassay of the leaves extract of Dillenia indica L. J Joung Pharm 2(1):50–53. https://doi.org/10.4103/0975-1483.62213
Hamrun N, Nabilah T, Hasyim R, Ruslin M, Dammar I, Arianto MA (2020) Toxicity test of bioactive red Alga extract Eucheumaspinosumon shrimp Artemia Salina leach. Sys Rev Pharm 11(5):672–676. https://doi.org/10.31838/srp.2020.5.94
Nerdy N, Lestari P, Sinaga J, Ginting S, Zebua N, Mierza V, Bakr T (2021) Brine, shrimp (Artemia salina Leach.) lethality test of ethanolic extract from green betel (Piper Betle Linn.) and red betel (Piper crocatum Ruiz and Pav.) through the soxhletation method for cytotoxicity. Maced J Med Sci 9(A):407–412. https://doi.org/10.3889/oamjms.2021.6171
Ferhat M, Erol E, Beladjila K, A, Çetintaş Y, Duru ME, Öztüc M, Kabouche A, Kabouche Z (2017) Antioxidant, anti-cholinesterase and antibacterial activities of Stachys Guyoniana. Pharm Biol 55(1):324–329. https://doi.org/10.1080/13880209.2016.1238488
Stegăruș DI, Lengyel E, Apostolescu GF, Botoran OR, Tanase C (2021) Phytochemical Analysis and Biological Activity of Three Stachys Species (Lamiaceae) from Romania. Plants. 9(10):2710. https://doi.org/10.3390/plants10122710. PMID: 34961181
Mrabti H, Bouyahya A, Ed-rac A, Kachmar MR, Mrabti N, Benali T, Shariati M, Ouahbi A, Doudach L, Faouzi M (2021) Polyphenolic profile and biological properties of Arbutus unedo root extracts. Eur J Integr Med 42:101266. https://doi.org/10.1016/j.eujim.2020.101266
Nouasri A, Ksouri A, Merah S, Metidji H, Krimat S (2022) Phytochemical study and antimicrobial activity of Cichorium intybus L. aqueous extracts. Analele Univ Din Oradea Fasc Biol 29(2):215–220
Belkacema N, Khettal B, Hudaib M, Bustanji Y, Abu-Irmaileh B, Amrine C (2021) Antioxidant, antibacterial, and cytotoxic activities of Cedrus Atlantica organic extracts and essential oil. Eur J Integr Med 42:101292. https://doi.org/10.1016/j.eujim.2021.101292
Ferrarini EG, Paes RS, Baldasso GM, de Assis PM, Gouvêa MC, Cicco P, Raposo NRB, Capasso R, Moreira ELG, Dutra RC (2022) Broad-spectrum cannabis oil ameliorates reserpine-induced fibromyalgia model in mice. Biomed Pharmacother 154:113552
Şahin TÖ, Yılmaz B, Yeşilyurt N, Cicia D, Szymanowska A, Amero P, Ağagündüz D, Capasso R (2023) Recent insights into the nutritional Immunomodulation of cancer-related MicroRNAs. Phytother Res 37(10):4375–4397
Cerqua I, Neukirch K, Terlizzi M, Granato E, Caiazzo E, Cicala C, Ialenti A, Capasso R, Werz O, Sorrentino R, Seraphin D, Helesbeux JJ, Cirino G, Koeberle A, Roviezzo F, Rossi A (2022) A vitamin E long-chain metabolite and the inspired drug candidate α-amplexichromanol relieve asthma features in an experimental model of allergen sensitization. Pharmacol Res 181:106250
Fernández J, Silván B, Entrialgo-Cadierno R, Villar CJ, Capasso R, Uranga JA, Lombó F, Abalo R (2021) Antiproliferative and palliative activity of flavonoids in colorectal cancer. Biomed Pharmacother 143:112241
Kocyigit E, Kocaadam-Bozkurt B, Bozkurt O, Ağagündüz D, Capasso R (2023) Plant toxic proteins: their biological activities, mechanism of action and removal strategies. Toxins (Basel) 15(6):356
Tareq AM, Hossain MM, Uddin M, Islam F, Khan Z, Karim MM, Lyzu C, Ağagündüz D, Reza ASMA, Emran TB, Capasso R (2023) Chemical profiles and Pharmacological attributes of Apis cerana indica beehives using combined experimental and computer-aided studies. Heliyon 9(4):e15016
Uddin Chy MN, Adnan M, Chowdhury MR, Pagano E, Kamal ATMM, Oh KK, Cho DH, Capasso R (2021) Central and peripheral pain intervention by Ophiorrhiza rugosa leaves: potential underlying mechanisms and insight into the role of pain modulators. J Ethnopharmacol 276:114182
Khan MF, Kader FB, Arman M, Ahmed S, Lyzu C, Sakib SA, Tanzil SM, Zim AFMIU, Imran MAS, Venneri T, Romano B, Haque MA, Capasso R (2020) Pharmacological insights and prediction of lead bioactive isolates of Dita bark through experimental and computer-aided mechanism. Biomed Pharmacother 131:110774
Laboratory of Bioactive Products and Biomass Valorization Research, ENS Kouba, BP92, Vieux-Kouba Alger, Algeria