Effects of drought stress and alternating temperature on seed quality parameters of Thymus munbyanus Boiss & Reut

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Research Articles | Published:

Print ISSN : 0970-4078.
Online ISSN : 2229-4473.
Pub Email: contact@vegetosindia.org
Doi: 10.1007/s42535-024-00904-5
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Keywords: n Thymus munbyanusn , Germination, Water stress, Temperature, PEG-6000


Thymus munbyanus Boiss & Reut, is an aromatic and medicinal plant, negatively affected by anthropogenic pressure and climate change. The objective of this research is to examine the effect of alternating temperature and PEG-induced drought stress on seed germination parameters and seedling vigor of T. munbyanus. Treatments included five alternating temperature regimes (10/0, 15/05, 20/10, 30/20, and 40/25°C), and five PolyEtylenGlycol6000–(PEG-6000) induced osmotic potentials (0, -0.1, -0.3, -0.6, and − 0.9 MPa). The Final germination percentage (FGP), the mean germination time (MGT) and Seedling vigor index I and II were assessed to examine the effect of alternating temperature, and drought stress. Our findings showed that temperature and PEG-induced drought stress significantly influence germination parameters and seedling vigor. In fact, the temperature 15/05°C registered the highest final germination percentage of 96%, the fast germination speed with a MGT of 4.3 ± 0.1 days and the best seedling vigor index I and II with values of 137.8 + 12.5 and 28.2 + 6.0 respectively. Germination parameters and seedling vigor are highest for low temperatures regimes; a very signification decrease is registered from 20/10°C to inhibition at alternating temperature 40/25°C. Drought stress results shows that the increase in PEG-6000 concentration affect negatively germination parameters and seedling vigor. In fact, germination is suppressed at the highest concentration (-0.9 MPa). These results are important for the restoration or rehabilitation of the species studied in degraded rangelands.

n                     Thymus munbyanusn                  , Germination, Water stress, Temperature, PEG-6000

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Abbad A, Belaqziz R, Bekkouche K, Markouk M (2011) Influence of temperature and water potential on laboratory germination of two Moroccan endemic thymes: Thymus maroccanus Ball. and Thymus broussonetii Boiss. Afr J Agric Res 6 (20):4740-5. https://doi.org/10.5897/AJAR10.574

Costa P, Gonçalves S, Valentão P, Andrade PB, Coelho N, Romano A (2012) Thymus lotocephalus wild plants and in vitro cultures produce different profiles of phenolic compounds with antioxidant activity. Food Chem 135(3):1253–1260. https://doi.org/10.1016/j.foodchem.2012.05.072

Darrudi R, Hassandokht MR, Nazeri V, Baradaran, Jafari A (2015) J Appl Res Med Aromat Plants 2 (4):168–173. doi: https://doi.org/10.1016/j.jarmap.2015.07.001

El Yaagoubi M, Mechqoq H, El Hamdaoui A, Mukku VJ, El Mousadik A, Msanda F, El Aouad N (2021) A review on Moroccan Thymus species: traditional uses, essential oils chemical composition and biological effects. J Ethnopharmacol 278:114205. https://doi.org/10.1016/j.jep.2021.114205

Elbouny H, Ouahzizi B, Bouhlali EDT, Sellam K, Alem C (2022) Pharmacological, biological and phytochemical aspects of Thymus munbyanus Boiss. & Reut.: a review. Plant Sci Today 9(2):399–404. https://doi.org/10.14719/pst.1494

Farooq M, Wahid A, Kobayashi N, Fujita D, Basra S (2009) Plant drought stress: effects, mechanisms and management. Sustain agric 153– 88. https://doi.org/10.1007/978-90-481-2666-8_12

Fennane M, Ibn Tattou M, Ouyahya A, El Oulaidi J (2007) Flore pratique du Maroc. Vol 2, Travaux Institut Scientifique, Sér. Botanique 38 (2):278

Flores J, Briones O (2001) Plant life-form and germination in a Mexican inter-tropical desert: effects of soil water potential and temperature. J Arid Environ 47(4):485–497. https://doi.org/10.1006/jare.2000.0728

Guan F, Liang Z, Huang L (2009) Principle of agro-biological management and countermeasure of agricultural industrialization on saline-alkalized land in Western Songnen Plain. Res Agric Moder 30(1):85–89. https://doi.org/10.1016/j.jaridenv.2008.08.009

Homrani BA, Mrabet R, Acherckouf M, Maatougui A (2020) Salsola vermiculata espèce prometteuse pour la réhabilitation des pâturages présahariens du Maroc. AFRIMED AJ - Al Awamia 128:143–157. https://doi.org/10.34874/IMIST.PRSM/afrimed-i128.31428

ISTA (2014) Seed vigour testing. International Seed Testing, AssociationPO Box 412, CH-8046 Zürich, Switzerland:333

Khoshsokhan F, Babalar M, Chaghazardi H, Fatahi Moghadam M (2011) Effect of salinity and drought stress on germination indices of two thymus species. Cer Agro Mold 45(1):27–35

Kumar V, Shahid M, Srivastava M, Singh A, Pandey S, Sharma A (2014) Enhancing seed germination and vigor of chickpea by using potential and effective strains of Trichoderma species. J Virol Mycol 3(2):2161–0517

Money NP (1989) Osmotic pressure of aqueous polyethylene glycols: relationship between molecular weight and vapor pressure deficit. Plant Physiol 91(2):766–769. https://doi.org/10.1104/pp.91.2.766

Morales R (2002) The history, botany and taxonomy of the genus Thymus. CRC press:15–57

Ouahzizi B, Elbouny H, Sellam K, Alem C, Bakali Homrani A (2023a) Effects of temperature, provenance, drought stress and salinity on seed germination response and early seedling stage of Thymus atlanticus (ball). Roussine J Appl Res Med Aromat Plants 34:100482. https://doi.org/10.1016/j.jarmap.2023.100482

Ouahzizi B, Elbouny H, Sellam K, Alem C, Homrani Bakali A (2022) Influence des températures alternées et la durée de stockage sur la germination des semences de Thymus satureioides. AFRIMED AJ - Al Awamia 135149–164. https://doi.org/10.34874/IMIST.PRSM/afrimed-i135.33277

Ouahzizi B, Elbouny H, Sellam K, Alem C, Homrani Bakali A (2023b) Effect of Salinity and Drought stresses on seed germination of Thymus satureioides. J Rangel sci 13(1):33–38

Ranal M, Santana DG (2006) How and why to measure the germination process? Rev Bras Bot 29:1–11. https://doi.org/10.1590/S0100-84042006000100002



Author Information

Ouahzizi Brahim
Biochemistry of Natural Products Team, Faculty of Sciences and Techniques, Moulay Ismail University, Errachidia, Morocco