Unfolding the Chemical composition, antioxidant and antibacterial activities of Drymaria cordata (Linn.) Willd. against chloramphenicol-resistant Bacillus subtilis and β-lactams-resistant Pseudomonas aeruginosa

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Doi: 10.1007/s42535-023-00673-7
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Keywords: n Drymaria cordatan , Bioactive compounds, Antioxidant activity, Antibacterial activity, Chemical composition


Abstract


The current study was done to investigate the chemical composition, antioxidant and antibacterial activities of Drymaria cordata. Different solvents i.e. methanol (DCM), hexane (DCH) and water (DCW) were used and compared for their antioxidant and antibacterial efficacy against chloramphenicol-resistant Bacillus subtilis and β-lactams-resistant Pseudomonas aeruginosa. GCMS chromatograms of three extracts depict the presence of antioxidant and antibacterial compounds such as hexanedioic acid, hexadecanoic acid, nonadecadiene, hexadecen-1-ol, octadecadienoic acid, nonane, phytol, henicosanal, stearyl aldehyde and neophytadiene. FTIR peaks of extracts were also analysed and showed the presence of phenols, amines, hydroxy groups, and amino-related components. The DPPH free radical scavenging assay exhibited the maximum percentage inhibition with DCM extract i.e. 71.57%. Also, DCM showed maximum total antioxidant capacity through the phosphomolybdate assay with values of 834.44 ± 4.01 mg AE/g of extract. For antibacterial activities, DCM showed the best zone of inhibition (ZOI) (8 mm at 100 µg/ml to 15.17 mm at 1000 µg/ml) and minimum inhibitory concentration (MIC) (75 µg/ml) as compared to other extracts against Bacillus subtilis. Also, DCM showed a zone of inhibition (ZOI) (6.83–13.17 mm) and a minimum inhibitory concentration (MIC) (70 µg/ml) as compared to other extracts against Pseudomonas aeruginosa. These results indicated that the Drymaria cordata methanol extract possesses good antibacterial and antioxidant properties which justify its use against pathogenic bacteria.


n              Drymaria cordatan            , Bioactive compounds, Antioxidant activity, Antibacterial activity, Chemical composition


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Acknowledgements


The authors are very thankful to the Principal, Professor Manoj K. Khanna, Ramjas College and the Head, Professor Suman Lakhanpaul, Department of Botany, University of Delhi, Delhi for providing the necessary facilities and encouragement during the investigation.


Author Information


Arya Atul
Medicinal Plants Research Laboratory, Department of Botany, Ramjas College, University of Delhi, Delhi, India

Kumar Suresh
Medicinal Plants Research Laboratory, Department of Botany, Ramjas College, University of Delhi, Delhi, India
suresh.kumar@ramjas.du.ac.in

Kain Dolly
Medicinal Plants Research Laboratory, Department of Botany, Ramjas College, University of Delhi, Delhi, India


Ahanger Ab Majeed
Medicinal Plants Research Laboratory, Department of Botany, Ramjas College, University of Delhi, Delhi, India


Suryavanshi Amrita
Medicinal Plants Research Laboratory, Department of Botany, Ramjas College, University of Delhi, Delhi, India

Vandana
Department of Chemistry, Dyal Singh College, University of Delhi, Delhi, India