Vegetos
(An International Journal of Plant Research & Biotechnology)
The performance of FTIR and UV-Vis spectroscopy combined with chemometrics to classify Impatiens balsamina L. leaf extracts from different solvent extraction
*Article not assigned to an issue yet
Research Articles | Published: 09 April, 2025
First Page: 0
Last Page: 0
Views: 231
Keywords: n Impatiens balsamina L. leaf, FTIR and UV-Vis spectroscopy, Chemometric, Aolvent extraction
Abstract
Impatiens balsamina L. is a home garden plant that has often been utilized by the community as a traditional medicine for painkillers and menstrual cramps. A previous study has successfully utilized this plant as an antimicrobial, antirheumatic, antipruritic, antiinflammation, anti-allergic, and antitumor agent. The bioactive compounds and the bioactivity of medicinal plants depend on several factors, like different solvent extraction. The combination of FTIR and UV-Vis spectra with chemometric technique becomes a choice to classify the I. balsamina leaf extract from different solvent extraction. Extraction was performed using a sonication method with the yield obtained from methanolic, ethyl-acetic, and chloroform extract were 31.93% ± 1.975, 16.20% ± 1.651, and 16.71% ± 1.742, respectively. The FTIR spectrum was measured at 600–4000 cm− 1 wavenumbers, while the UV-Vis spectrum was measured at 200–280 nm wavelengths. The I. balsamina leaf extracts obtained a similar absorbance value in FTIR for ethyl-acetic and chloroform extracts, but different from methanolic extract. The PCA grouping was performed for further classification assisted by orange data mining software. The total value of PC from UV-Vis spectra was 90% and the total value of PC from FTIR spectra was 85%. Therefore, the I. balsamina leaf extract can be grouped based on different solvent extractions. A confusion matrix analysis presents few samples of ethyl-acetic extract are described as chloroform extract, and vice versa, following the PCA results from either FTIR or UV-Vis spectra.
References
Adfa M (2008) Antibacterial compound from impatiens balsamina Linn leaf. Jurnal Gradien 4(1):318–322
Agustika DK, Mercuriani I, Purnomo CW, Hartono S, Triyana K, Iliescu DD, Leeson MS (2022) Fourier transform infrared spectrum pre-processing technique selection for detecting PYLCV-infected Chilli plants. Spectrochimica Acta - Part A: Mol Biomol Spectrosc 278. https://doi.org/10.1016/j.saa.2022.121339
Aziz Z, Yuliana ND, Simanjuntak P, Rafi M, Syamsudin (2020) FTIR and HPLC-based metabolomics of Yacon leaves extracts (Smallanthus Sonchifolius [poepp & endl.] h. robinson) from two locations in Indonesia. Indonesian J Chem 20(3):567–578. https://doi.org/10.22146/ijc.43453
Ebrahimi-Najafabadi H, Leardi R, Oliveri P, Chiara Casolino M, Jalali-Heravi M, Lanteri S (2012) Detection of addition of barley to coffee using near infrared spectroscopy and chemometric techniques. Talanta 99:175–179. https://doi.org/10.1016/j.talanta.2012.05.036
El Mrabet A, El Orche A, Diane A, Johnson JB, Haj Said A, Bouatia A, M., Sbai-Elotmani I (2024) Rapid analysis of Eucalyptus oil adulteration in Moroccan Rosemary essential oil via GC-FID and mid-infrared spectroscopy. Vib Spectrosc 132(March):103674. https://doi.org/10.1016/j.vibspec.2024.103674
Hariyanto I, Fajriaty I, Wijaya T, Hafizh M (2018) The potential ethnomedicine plant of impatiens balsamina leaves from Pontianak, West Kalimantan, Indonesia for wound healing. Nusantara Bioscience 10(1):58–64. https://doi.org/10.13057/nusbiosci/n100109
Jiang H, Yang L, Xing X, Yan M, Guo X, Yang B, Wang Q, Kuang H (2018) HPLC-PDA combined with chemometrics for quantitation of active components and quality assessment of Raw and processed fruits of xanthium strumarium L. Molecules 23(243):1–16. https://doi.org/10.3390/molecules23020243
Jollife IT, Cadima J (2016) Principal component analysis: A review and recent developments. Philosophical Trans Royal Soc A: Math Phys Eng Sci 374(2065). https://doi.org/10.1098/rsta.2015.0202
Kang SN, Goo YM, Yang MR, Ibrahim RIH, Cho JH, Kim IS, Lee OH (2013) Antioxidant and antimicrobial activities of ethanol extract from the stem and leaf of impatiens balsamina L. (balsaminaceae) at different harvest times. Molecules 18(6):6356–6365. https://doi.org/10.3390/molecules18066356
Kautsar A, Wahyuni WT, Syafitri UD, Muflihah S, Mawadah N, Rohaeti E, Arif Z, Prajogo B, Amran MB, Rohman A, Rafi M (2021) Data fusion of UV-Vis and FTIR spectra combined with principal component analysis for distinguishing of andrographis paniculata extracts based on cultivation ages and solvent extraction. Indonesian J Chem 21(3):753–760. https://doi.org/10.22146/ijc.60321
Lopez-Vidal S, Rodriguez-Estevez V, Lago S, Arce L, Valcarel M (2008) The application of GC– MS and chemometrics to categorize the feeding regime of Iberian pigs in Spain. Chromatographia 68(October):593–601. https://doi.org/10.1365/s10337-008-0752-x
Mabry TJ, Markham KR, Thomas MB (1970) The ultraviolet spectra of flavones and flavonols. Syst Identif Flavonoids See 111:41–164. https://doi.org/10.1007/978-3-642-88458-0_5
Nugroho A, Gumelar AB, Sooai AG, Sarvasti D, Tahalele PL (2020) Comparison of the performance of guided classification algorithms for cardiovascular disease cases. Jurnal Resti 5(3):998–1006
Purwakusumah ED, Rafi M, Syafitri UD, Nurcholis W, Agung M, Adzkiya Z (2014) Identification and authentication of Jahe Merah using combination of FTIR spectrocopy and chemometrics. J Indonesian Chem Soc 34(1):82–87
Rachmawati, Rohaeti E, Rafi M (2017) Combination of near infrared spectroscopy and chemometrics for authentication of taro flour from wheat and sago flour. Journal of Physics: Conference Series, 835(1). https://doi.org/10.1088/1742-6596/835/1/012011
Rafi M, Devi AF, Syafitri UD, Heryanto R, Suparto IH, Amran MB, Rohman A, Prajogo B, Lim LW (2020) Classification of andrographis paniculata extracts by solvent extraction using HPLC fingerprint and chemometric analysis. BMC Res Notes 13(1):56. https://doi.org/10.1186/s13104-020-4920-x
Rafi M, Purwakusumah ED, Ridwan T, Barus B (2015) Geographical classification of Java tea (Orthosiphon stamineus) from Java Island by FTIR spectrocopy combined with canonical variate analysis. Jurnal Sains Dan Matematika 23(1):25–31
Rohaeti E, Muzayanah K, Septaningsih DA, Rafi M (2019) Fast analytical method for authentication of Chili powder from synthetic dyes using UV-Vis spectroscopy in combination with chemometrics. Indonesian J Chem 19(3):668–674. https://doi.org/10.22146/ijc.36297
Sharief TM (2022) Comparison of different solvent and extraction methods for isolation of flavonoids compound from leaves of clerodendrum infortunatum Linn. J Plant Biochem Physiol 10(1000283):1–2. https://doi.org/10.35841/2329-9029.22.10.283
Suendo V, Sandiningtyas RD (2010) Isolation of chlorophyll a from spinach and its modification using Fe2 + in photostability study Plasma modified polyaniline counter electrode for DSSC applications. Proceedings of the Third International Conference on Mathematics and Natural Sciences (ICMNS 2010), January 2010. https://www.researchgate.net/publication/265490978
Tošović J, Marković S (2017) Reproduction and interpretation of the UV-vis spectra of some flavonoids. Chem Pap 71(3):543–552. https://doi.org/10.1007/s11696-016-0002-x
Triawan DA, Ratnawati D, Nurwidiyani R, Notriawan D, Adfa M, Bahar RS, Rafi M (2024) Data fusion of FTIR and UV–Vis spectra combined with chemometrics to classification garden Balsam leaves (Impatiens Balsamina L.) extract from two different location in Bengkulu Province. Vegetos, p 0123456789. https://doi.org/10.1007/s42535-024-00960-x
Wahyuni WT, Saharah M, Arif Z, Rafi M (2020) Thin layer chromatographic fingerprint and chemometrics analysis for identification of phyllanthus Niruri from its related species. J Indonesian Chem Soc 3(1):47. https://doi.org/10.34311/jics.2020.03.1.47
Wulandari L, Permana BD, Kristiningrum N (2020) Determination of total flavonoid content in medicinal plant leaves powder using infrared spectroscopy and chemometrics. Indonesian J Chem 20(5):1044–1051. https://doi.org/10.22146/ijc.47047
Author Information
Science Laboratory, Faculty of Mathematics and Natural Sciences, University of Bengkulu, Bengkulu, Indonesia