Vegetos
(An International Journal of Plant Research & Biotechnology)
The improvement of PCR amplification pattern by PCR enhancers and formulation novel PCR enhancer
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Short Communications | Published: 31 December, 2024
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Keywords: PCR enhancers, PCR additives, Enzyme stabilizing compounds, Novel PCR enhancer, Sugars and Sugar alcohols
Abstract
PCR protocols are always in need of improvement for scientific and industrial analysis. Numerous compounds have been shown to improve PCR amplification and function as PCR enhancers. Majority of PCR enhancement research used agarose gel electrophoresis to measure each enhancers concentration. The visual comparative observation is limited in the agarose gel electrophoresis, to facilitate the visual comparative analysis and to draw a conclusion, a computer-based gel analysis software was used. In the present study, eighteen enhancers were grouped into eight categories and tested in a range of concentrations. Among them, Glycerol and Trehalose were the only known kinds of sugars and sugar alcohols used as a PCR enhancers. We documented how eleven sugars and sugar alcohols enhance PCR amplification and reported that some of them were remarkably effective, often surpassing eighteen known PCR enhancers. We formulated novel PCR enhancers (NPEs) that greatly enhance the PCR process by lowering the melting temperature of DNA and thermostabilizing Taq DNA polymerase. A novel PCR enhancer is a beneficial and economically appealing for large-scale projects and routine applications that require reliable PCR results.
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Author Information
Department of Microbiology, Faculty of Science, Marwadi University, Rajkot, India