Temporal dynamics of enzyme-driven polyphenol modifications in stored eggplant (Solanum melongena L.)

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Print ISSN : 0970-4078.
Online ISSN : 2229-4473.
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Doi: 10.1007/s42535-024-00847-x
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Keywords: Antioxidant, Anthocyanins, Brinjal, Polyphenol oxidase, Peroxidase, Postharvest storage, Phenolics


Abstract


The Postharvest handling of eggplants (Solanum melongena L.) significantly affects their metabolic profile and enzymatic response. Limited information is available regarding the enzymatic or oxidative degradation of polyphenols in eggplants and their free radical scavenging potential (FRSP) under variable storage temperature. The present study was conducted on postharvest storage of eggplant fruits under ambient (AS) and cold storage (CS) conditions. Polyphenol content and degradation products were evaluated in eggplant fruits using biochemical methods, whereas the FRSP was analysed using DPPH, ABTS, and FRAP assays. Polyphenol oxidase (PPO) and peroxidase (POD) were key enzymes involved in the polyphenol degradation of eggplant. PPO (18.4–53%) and POD (9.8%) activities were reduced under cold storage as compared to the ambient storage conditions. Oxidative enzyme activities and FRSP were correlated to the phenolic content under AS and CS conditions. Storage of eggplants at CS conditions reduced the phenolics degradation significantly.


Antioxidant, Anthocyanins, Brinjal, Polyphenol oxidase, Peroxidase, Postharvest storage, Phenolics


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References


Alsawmahi ON, Al-Juhaimi F, Alhamdan AM et al (2018) Phenolic, tannin, antioxidant, color, and sensory attributes of Barhi date (Phoenix dactylifera) fruit stored in modified atmosphere packages. J Food Biochem E 12576. https://doi.org/10.1111/jfbc.12576


Antony A, Farid M (2022) Effect of temperatures on polyphenols during extraction. Appl Sci 12:2107. https://doi.org/10.3390/app12042107


Aryal S, Baniya MK, Danekhu K, Kunwar P, Gurung R, Koirala N (2019) Total phenolic content, flavonoid content and antioxidant potential of wild vegetables from western Nepal. Plants 8:96. https://doi.org/10.3390/plants8040096


Balbaa SI, Zake AY, Elshamy AM (1974) Total flavanoid and rutin content of the different organs of Sophora japonica L. J Assoc off Anal Chem 57:752–755. https://doi.org/10.1093/jaoac/57.3.752


Concellón A, Zaro MJ, Chaves AR, Vicente AR (2012) Changes in quality and phenolic antioxidants in dark purple American eggplant (Solanum melongena L. Cv. Lucía) as affected by storage at 0°C and 10°C. Postharv Biol Technol 66:35–41. https://doi.org/10.1016/j.postharvbio.2011.12.003


Barragan J, Franco A, López J, Perez-Cervera C (2019) Effect of Storage conditions on Physicochemical characteristics and Phenolic compounds of Eggplant (Solanum melongena L). Revista De Ciencias Agrícolas 36(2):5–16. https://doi.org/10.22267/rcia.193602.114


Braga PC, Scalzo RL, Dal Sasso M, Lattuada N, Greco V, Fibiani M (2016) Characterization and antioxidant activity of semi-purified extracts and pure delphinidin-glycosides from eggplant peel (Solanum melongena L). J Funct Foods 20:411–421. https://doi.org/10.1016/j.jff.2015.10.032


Kappel N, Mozafarian M (2022) Effects of different rootstocks and Storage temperatures on Postharvest Quality of Eggplant (Solanum melongena L. Cv. Madonna) Hortic 8(10):862. https://doi.org/10.3390/horticulturae8100862


Concellon A, Anon MC, Chaves AR (2007) Effect of low temperature storage on physical and physiological characteristics of eggplant fruit (Solanum melongena L). Food Sci Technol 40:389–396. https://doi.org/10.1016/j.lwt.2006.02.004


Muche BM, Speers RA, Rupasinghe HV (2018) Storage temperature impacts on anthocyanins degradation, color changes and haze development in juice of Merlot and Ruby grapes (Vitis vinifera). Front Nut 5:100. https://doi.org/10.3389/fnut.2018.00100


Colak N, Kurt-Celebi A, Gruz J, Strnad M, Hayirlioglu -Ayaz S, Choung MG, Esatbeyoglu T, Ayaz FA (2022) The phenolics and antioxidant properties of black and purple versus white eggplant cultivars. Molecules 27:2410. https://doi.org/10.3390/molecules27082410


Compton DL, Laszlo JA, Evans KO (2012) Antioxidant properties of feruloyl glycerol derivatives. Ind Crops Prod 36. https://doi.org/10.1016/j.indcrop.2011.09.009.:217– 21


Enachi E, Grigore-Gurgu L, Aprodu I, Stănciuc N, Dalmadi I, Bahrim G, Râpeanu G, Croitoru C (2018) Extraction, purification and processing stability of peroxidase from plums (Prunus domestica). Int J Food Prop 21(1):2744–2757. https://doi.org/10.1080/10942912.2018.1560311


Enaru B, Dretcanu G, Pop TD, Stanil A, Diaconeasa Z (2021) Anthocyanins: factors affecting their Stability and Degradation. https://doi.org/10.3390/antiox10121967. Antioxidants 10:1967


Fu R, Zhang YT, Guo YR, Huang QL, Peng T, Xu Y, Tang L, Chen F (2013) Antioxidant and anti-inflammatory activities of the phenolic extracts of Sapium sebiferum (L.) leaves. J Ethnopharmac 147:517–524. https://doi.org/10.1016/j.jep.2013.03.058


Galani JHY, Patel JS, Patel NJ, Talati JG (2017) Storage of fruits and vegetables in Refrigerator increases their phenolic acids but decreases the total phenolics, anthocyanins and Vitamin C with subsequent loss of their antioxidant capacity. Antioxidants 6(3):59. https://doi.org/10.3390/antiox6030059


Güllçin I, Küfreviog˘lu OI, Oktay M (2005) Purification and characterization of polyphenol oxidase from nettle (Urtica dioica L.) and inhibitory effects of some chemicals on enzyme activity. J Enzyme Inhib Med Chem 20:297–302. https://doi.org/10.1080/1475636032000141890


Ismail A, Marjan ZM, Foong CW (2004) Total antioxidant activity and phenolic content in selected vegetables. Food Chem 87:581–586. https://doi.org/10.1016/j.foodchem.2004.01.010


Jenkins K, Diehl SV, Clausen CA, Green F (2011) Enzymatic mechanism of oxalate production in the TCA and glyoxylate pathways using various isolates of Antrodia radiculosa. In: Proceedings, one hundred seventh annual meeting of the American Wood Protection Association, Fort Lauderdale, FL. 2011 May 15–17: Volume 107. Birmingham, ALa: American Wood Protection Association, c2011: pp. 108–113


Joët T, Salmona J, Laffargue A, Descroix F, Dussert S (2010) Use of the growing environment as a source of variation to identify the quantitative trait transcripts and modules of co-expressed genes that determine chlorogenic acid accumulation. Plant Cell Env 33:1220–1233. https://doi.org/10.1111/j.1365-3040.2010.02141.x


Liu F, Zhao JH, Wen X, Ni YY (2015) Purification and structural analysis of membrane-bound polyphenol oxidase from Fuji apple. Food Chem 183:72–77. https://doi.org/10.1016/j.foodchem.2015.03.027


Madinez MV, Whitaker JR (1995) The biochemistry and control of enzymatic browning. Trend Food Sci Technol 6:195–200. https://doi.org/10.1016/S0924-2244(00)89054-8


Mahadevan A (1979) Physiology of host–parasite interaction. Today and Tomorrow Publication, New Delhi. 167– 71


Mahadevan A, Sridhar R (1986) In: Methods in Physiological Plant Pathology (3rd edn.), Sivakami Publications, Chennai 189–190


Massolo JF, Concellón A, Chaves AR, Vicente AR (2011) 1-Methylcyclopropene (1-MCP) delays senescence, maintains quality and reduces browning of nonclimacteric eggplant (Solanum melongena L.) fruit. Postharv Biol Technol 59:10–15. https://doi.org/10.1016/j.postharvbio.2010.08.007


Mayer AM (2006) Polyphenol oxidases in plants and fungi: going places? A review. Phytochem 67:2318–2331. https://doi.org/10.1016/j.phytochem.2006.08.006


Mayer AM, Harel E (1979) Polyphenol oxidase in plants. Phytochem 18:193–215. https://doi.org/10.1016/0031-9422(79)80057-6


Mendes L, de Freitas V, Baptista P, Carvalho M (2011) Comparative antihaemolytic and radical scavenging activities of strawberry tree (Arbutus unedo L.) leaf and fruit. Food Chem Toxicol 49:2285–2291. https://doi.org/10.1016/j.fct.2011.06.028


Mennella G, Lo Scalzo R, Fibiani M, D’Alessandro A, Francese G, Toppino L, Acciarri N, de Almeida AE, Rotino GL (2012) Chemical and bioactive quality traits during fruit ripening in eggplant (Solanum melongena L.) and allied species. J Agric Food Chem 60:11821–11831. https://doi.org/10.1021/jf3037424


Moon KM, Kwon EB, Lee B, Kim CY (2020) Recent trends in controlling the enzymatic browning of fruit and vegetable products. Molecules 25:2754. https://doi.org/10.3390/molecules25122754


Mora J, Pott DM, Osorio S, Vallarino JG (2022) Regulation of Plant Tannin Synthesis in Crop species. Front Genet 13:870976. https://doi.org/10.3389/fgene.2022.870976


Ofoedu CE, You L, Osuji CM, Iwouno JO, Kabuo NO, Ojukwu M, Agunwah IM, Chacha JS, Muobike OP, Agunbiade AO (2021) Hydrogen Peroxide effects on Natural-Sourced polysacchrides: free radical Formation/Production, degradation process, and reaction Mechanism—A critical synopsis. Foods 10:699. https://doi.org/10.3390/foods10040699


Patras A, Brunton NP, O’Donnell C, Tiwari BK (2010) Effect of thermal processing on anthocyanin stability in foods; mechanisms and kinetics of degradation. Trend Food Sci Technol 21:3–11. https://doi.org/10.1016/j.tifs.2009.07.004


Pérez-Gilabert M, Carmona FG (2000) Characterization of catecholase and cresolase activities of eggplant polyphenol oxidase. J Agric Food Chem 48:695–700. https://doi.org/10.1021/jf990292r


Raigón MD, Prohens J, Muñoz-Falcón JE, Nuez F (2008) Comparison of eggplant landraces and commercial varieties for fruit content of phenolics, minerals, dry matter and protein. J Food Compost Anal 21:370–376. https://doi.org/10.1016/j.jfca.2008.03.006


Reddy CVK, Sreeramulu D, Raghunath M (2010) Antioxidant activity of fresh and dry fruits commonly consumed in India. Food Res Int 43:285–288. https://doi.org/10.1016/j.foodres.2009.10.006


Sadasivam S, Manickam A (1992) Biochemical methods for Agricultural sciences. WILEY Eastern Ltd., New Delhi 189– 91


Shannon LM, Kay E, Lew JY (1966) Peroxidase isozymes from horseradish roots: I. isolation and physical properties. J Biol Chem 241:2166–2172. https://doi.org/10.1016/S0021-9258(18)96680-9


Sharma H, Chawla N, Dhatt AS (2019) Nutraceutical content and free radical scavenging capacity of brinjal (Solanum melongena L.) genotypes. Sci Hort 244:294–303. https://doi.org/10.1016/j.scienta.2018.09.060


Sharma H, Chawla N, Dhatt AS (2022) Role of phenylalanine/tyrosine ammonia lyase and anthocyanidin synthase enzymes for anthocyanin biosynthesis in developing Solanum melongena L. genotypes. https://doi.org/10.1111/ppl.13756. Physiol Plant e13756


Sharma H, Chawla N, Dhatt AS (2023) Utilizing response surface methodology to optimize the extraction and characterization of anthocyanins from Solanum melongena L. fruit. Nat Prod Res 1–8. https://doi.org/10.1080/14786419.2023.2244137


Shetty SM, Chandrashekar A, Venkatesh YP (2011) Eggplant polyphenol oxidase multigene family: Cloning, phylogeny, expression analyses and immunolocalization in response to wounding. Phytochem 72:2275–2287. https://doi.org/10.1016/j.phytochem.2011.08.028


Silva EM, Souza JNS, Rogez H, Rees JF, Larondelle Y (2007) Antioxidant activities and polyphenolic contents of fifteen selected plant species from the amazonian region. Food Chem 101:1012–1018. https://doi.org/10.1016/j.foodchem.2006.02.055


Swain T, Hillis WE (1959) The phenolic constituents of Prunus domestica I. The quantitative analysis of phenolic constituents. J Sci Food Agric 10:63–68. https://doi.org/10.1002/JSFA.2740100110


Tan C, Dadmohammadi Y, Lee MC, Abbaspourrad A (2021) Combination of copigmentation and encapsulation strategies for the synergistic stabilization of anthocyanins. Compr Rev Food Sci Food Saf 20:3164–3191. https://doi.org/10.1111/1541-4337.12772


Tao W, Pan H, Jiang H, Wang M, Ye X, Chen S (2022) Extraction and identification of proanthocyanidins from the leaves of persimmon and loquat. Food Chem 372:130780. https://doi.org/10.1016/j.foodchem.2021.130780


Thaipong K, Boonprakob U, Crosby K, Cisneros-Zevallos L, Byrne DH (2006) Comparison of ABTS, DPPH, FRAP, and ORAC assays for estimating antioxidant activity from guava fruit extracts. J Food Compos Anal 19:669–675. http://refhub.elsevier.com/S0304-4238(18)30678-2/sbref027


Torun H, Kolcuoglu Y, Ayaz FA, Çolak A, Glew RH (2015) Characterization of polyphenol oxidase during three ripening stages of an eggplant (Solanum melongena L.) fruit: a local type in northeast Anatolia. Turk J Biochem 40:44–50. https://doi.org/10.5505/tjb.2015.27146


Zauberman G, Ronen R, Akerman M, Wrksler A, Rot I, Fuchs Y (1991) Postharvest retention of the red color of litchi fruit pericarp. Sci Hort 47:89–97. https://doi.org/10.1016/0304-4238(91)90030-3


Zhang Y, Truzzi F, D’Amen E, Dinelli G (2021) Effect of storage conditions and time on the polyphenol content of wheat flours. Processes 9:248. https://doi.org/10.3390/pr9020248

 


Acknowledgements



Author Information


Sharma Himanshu
Department of Biochemistry, Punjab Agricultural University, Ludhiana, India
himanshu.pau@gmail.com
Chawla Neena
Department of Vegetable Science, Punjab Agricultural University, Ludhiana, India


Dhatt Ajmer Singh
Department of Vegetable Science, Punjab Agricultural University, Ludhiana, India