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Chandola Vaishali, Chandra Sudeep, Nautiyal A. R., Kumar Rajeev Ranjan, Purohit V. K.
Keywords:
In vitro propagation, Multiple shoot formation, n Trillium govanianumn , TDZ, Zeatin
Trillium govanianum, locally known as “Nagchattri”, is an endangered alpine herb and a potent source of plant steroids. Its unabated exploitation and limited seed production are the major threats to its sustainability and calls for methods of conservation. The present study thus focuses on vegetative propagation in T. govanianum and reports effective and repeatable in vitro propagation protocol for multiple shoot proliferation and plantlet formation in it. The study involves the use of biotechnological tools and exogenously supplied plant growth regulators to overcome dormancy in in vitro cultured apical buds of T. govanianum resulting in multiple shoot proliferation. The study also throws light on propagule harvesting stage and chamber conditions for successful proliferation of T. govanianum. Apical buds from dormant period were found more responsive than apical buds from emergence period. Onset of shoot initiation took a minimum of 20 d to a maximum of 54 d whereas complete shoot formation took a minimum of 43 d to a maximum of 96 d. Thidiazuron (TDZ) and Zeatin (Zea) were found to be effective at 4 and 5 mgL-1. Maximum shooting percentage (75%) was observed in the bud explants from dormant period. Maximum shoot formation (3 ± 1) and maximum shoot length (1.8 ± 0.02 cm) was observed in MS media with Zeatin (4 and 5 mgL-1) in dormant buds. Maximum root proliferation (88.9 ± 0.6%) was observed in IAA + TDZ (3 + 1 mgL-1) and took an average of 65 ± 5 d. The results of this study can be employed for producing T. govanianum plants and its efficient in vitro culture and conservation.
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The authors would like to acknowledge the Director, laboratory and field staff of HAPPRC at Srinagar and Alpine Research Station Tungnath for providing the facilities and help during the present work.