Statins mimic and free radical scavenging potential of phytoconstituents of methanolic pod extract of Prosopis cineraria (L.) Druce

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Print ISSN : 0970-4078.
Online ISSN : 2229-4473.
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Doi: 10.1007/s42535-023-00677-3
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Keywords: Antioxidants, Quercetin, HMG-CoA reductase, Hypercholesterolemia, Phytocompounds, n Prosopis cinerarian


Abstract


The current study aimed to investigate statin (HMG-CoA reductase inhibitor) mimic and free radical scavenging potential of phytoconstituents of the methanolic extract of Prosopis cineraria pod by in vitro, in silico, and in vivo assessments. The phytoconstituents of the chosen extract were screened out by using the LCMS and GCMS analyses. The in vitro assessment of the extract shown 78.3% (IC50 1.7786 μg/ml) inhibition of HMG-CoA reductase as compared with pravastatin (positive control). The administration of the extract made considerable (P ≤ 0.001) improvements in lipid profile, atherogenic indices and oxidative stress. The in silico studies of molecular docking revealed significant binding energy of key identified compounds obtained in test extracts, i.e., cinnerin C, prosogerin A, prosogerin B, prosopine, spicegrin, quercetin, alpha-d-mannofuranoside, methyl, undecanoic acid and l-gala-l-iodo-octose with target enzymes. The quercetin showed significant interaction with the target protein (HMG-CoA reductase) compared to atorvastatin with − 8.4 kcal/mol binding energy. Further, validations of ligands-protein interactions were examined by molecular dynamics at 100 ns through RSMD, RSMF, SASA and MMPBSA, which revealed the significant interactions of quercetin and atorvastatin with HMGCR. ADMET predictions shown significant pharmacokinetic profiles of phytoconstituents. The phytoconstituents of Prosopis cineraria pod of methanolic extract contains potent phytochemicals like quercetin which may be concerned to the inhibition of HMG-CoA reductase and free radical scavenging activities.


Antioxidants, Quercetin, HMG-CoA reductase, Hypercholesterolemia, Phytocompounds, n              Prosopis cinerarian


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Acknowledgements


We want to acknowledge the kind cooperation of AIIMS, Jodhpur, for the HMG-CoA reductase inhibition assay and the Department of Zoology for providing essential support for this study.


Author Information


Jaipal Noopur
Department of Zoology, Faculty of Science, Jai Narain Vyas University, Jodhpur, India

Ram Heera
Department of Zoology, Faculty of Science, Jai Narain Vyas University, Jodhpur, India
hr.zo@jnvu.edu.in

Kumar Pramod
Department of Zoology, Faculty of Science, Jai Narain Vyas University, Jodhpur, India


Charan Jaykaran
Department of Pharmacology, All India Institute of Medical Sciences, Jodhpur, India


Kashyap Priya
University School of Biotechnology, GGS Indraprastha University, New Delhi, India

Chowdhury Suman
University School of Biotechnology, GGS Indraprastha University, New Delhi, India

Tripathi Rashmi
Department of Bioscience and Biotechnology, Banasthali Vidyapith, Banasthali, India

,
Kumar Shivani
University School of Biotechnology, GGS Indraprastha University, New Delhi, India

Singh Bhim Pratap
Department of Agriculture and Environmental Sciences, National Institute of Food Technology Entrepreneurship and Management (NIFTEM), Sonipat, India
bpsingh@niftem.ac.in
Panwar Anil
Department of Bioinformatics and Computational Biology, CCS Haryana Agricultural University, Hisar, India