Kumar Anuj, Gond S K, Mishra A, Sharma V K, Verma S K, Kharwar R N*
Keywords:
Soil mycoflora, CFU, Media pH, Distribution pattern, Diversity.
During present study, 513 isolates were recovered from 15 different experimental sites. For fungal recovery, PDA medium proved better over Czapek s dox agar medium (CDA), while Waksman dilution method showed higher isolates recovery than Warcup s soil plate method. Thirty filamentous fungi were recovered of which Aspergillus, Alternaria, Cladosporium, and Fusarium were dominant. Aspergillus niger and A. fumigatus showed a high frequency of occurrence and were isolated @ 80 x103 CFU/g and 47x 103 CFU/g, respectively from dry, moist and humid soil samples, while Penicillium notatum (37x103 CFU/g), Nigrospora oryzae (30 x103 CFU/g), Rhizopus nigricans (32x103 CFU/g), Trichoderma viride (24x103 CFU/g) were isolated with moderate frequency. Fifty three isolates were recovered from site 10 (Agriculture field, BHU) which was highest whereas, 12 isolates were recovered from site 1 (Cantt Railways Station). Consequently, the site 10 had the maximum CFU value (53x103 CFU/g) whereas site 1 had the lowest (12 x103 CFU/g). A higher percentage of soil fungi isolated have preferred the acidic pH range. Mean of fungal isolates was higher in moist (37.60), followed by humid (34.50) and dry (30.20) conditions. Bray-Curtis cluster analysis was done to understand that several variables are responsible for the diversity and occurrence of soil fungi.
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*Corresponding author Email: rnkharwar@yahoo.com