Vegetos
(An International Journal of Plant Research & Biotechnology)
Regeneration potential of Ascocentrum ampullaceum (Roxb.) Schlter through inflorescence culture
Research Articles | Published: 03 April, 2017
Online ISSN : 2229-4473.
Website:www.vegetosindia.org
Pub Email: contact@vegetosindia.org
First Page: 46
Last Page: 49
Views: 1186
Keywords:
<I>Ascocentrum ampullaceum</I>, Callus, Inflorescence, <I>In vitro</I> culture, Nutrient media, Protocorm like bodies, Shoot formation.
Abstract
The paper deals with the in vitro regeneration response of immature inflorescence segments (buds, nodes and internodes) of Ascocentrum ampullaceum explants. The nutrient medium used played a key role in induction and multiplication of callus, protocorm like bodies and shoot. Inflorescence buds cultured in V&W medium supplemented with 15% coconut milk and 2% sucrose produced highest (80%) protocorm like bodies formation without any growth regulator. Inflorescence node culture in MKC medium supplemented with only 15% CM, and 15% CM and 1.0 mg l−1 NAA produced highest callus (70%) and plbs (50%) formation, respectively. Incorporation of 15% CM in all the media proved best for formation of callus and plbs. Our results suggest that Ascocentrum ampullaceum, which regenerates poorly in nature, can be micropropagated using inflorescence based explants.
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References
Acknowledgements
Author Information
1State Forest Research Institute, Itanagar, Arunachal Pradesh, India
*Corresponding author: Padmanabh Dwivedi, Department of Plant Physiology, Institute of Agricultural Sciences, Baranas Hindu University, Varanasi, India, Tel: 0542-6701112; E-mail: pdwivedi25@rediffmail.com