Quantification of β -ODAP content in seeds of Lathyrus aphaca L: a minor crop species of local importance

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Online ISSN : 2229-4473.
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Doi: 10.1007/s42535-023-00734-x
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Keywords: n Lathyrus aphacan , HPLC, β-ODAP, Neurolathyrism


Abstract


Lathyrus aphaca L. is a crop of rabi season with a strong potential both as human food, animal feed and fodder. It contains a non-protein amino acid β -ODAP which causes degeneration of motor neurons i.e., neurolathyrism. A total of 10 populations growing in different areas of Jammu region of J&K UT and its adjoining areas were selected and subjected to HPLC for the quantification of their respective β-ODAP contents. Of these 10, three grouped into one subcluster having low quantity of β-ODAP. The plants of these populations will be useful in breeding programmes and can be further used as a substitute for green pea.


n              Lathyrus aphacan            , HPLC, β-ODAP, Neurolathyrism


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References


Aarssen LW, Hall IV, Jensen KIN (1986) The biology of Canadian weeds. 76. Vicia augustifolia L., Vicia cracca L., Vicia sativa L, Vicia tetrasperma (L.) Schreb and Vicia villosa Roth. Can J Plant Sci 66:711–737


Abbas G, Ali MA, Abbas Z, Aslam M, Akram M (2009) Impact of different herbicides on broadleaf weeds and yield of wheat. Pakistan J Weed Sci Resour 15(1):1–10


Ali HBM, Meister A, Schubert I (2000) DNA content, rDNA loci, and DAPI bands reflect the phylogenetic distance between Lathyrus species. Genome 43:1027–1032


Altundag E, Ozturk M (2011) Ethnomedicinal studies on the plant resources of east Anatolia, Turkey. Proc Soc Behav Sci 19:756–777


Bell EA (2003) (2003) Nonprotein amino acids of plants: significance in medicine, nutrition, and agriculture. J Agric Food Chem 51:2854–2865


Bento-Silva A, Gonçalves L, Mecha E, Pereira F, Vaz Patto MC, Bronze M (2019) An improved HILIC HPLC-MS/MS method for the determination of β-ODAP and its α isomer in Lathyrus sativus. Molecules (Basel, Switzerland) 24(17):3043. https://doi.org/10.3390/molecules24173043


Denes A, Papp N, Babal D, Cruz B, Molnar Z (2012) Wild plants used for food bu Hungarian ethnic groups living in the Carpathian Basin. Acta Soc Bot Pol 81(4):381–396


Ehara K (1950) Forage crops I. Yokendo, Tokyo, pp 444–452


El-Moneim, AMA., Van Dorrestein, B., Baum, M., Bejiga, J.R.G. (2001). Role of ICARDA in improving the nutritional quality and yield potential of grasspea (Lathyrus sativus L.), for subsistence farmers in dry areas. Lathyrus


Enneking D (2011) The nutritive value of grasspea (Lathyrus sativus) and allied species, their toxicity to animals and the role of malnutrition in neurolathyrism. Food Chem Toxicol 49:694–709


Fikre A, Negwo T, Kuo Y-H, Lambein F, Ahmed S (2011) Climatic, edaphic and altitudinal factors a_ecting yield and toxicity of Lathyrus sativus grown at five locations in Ethiopia. Food Chem Toxicol 49:623–630


Gams H (1924) Lathyrus L. In: Hegi G (ed) Illustrierte Flora von Mittepleuropa issue no 3, vol 4. Lehmann, Munchen, pp 1494–1506


Ikegami F, Itagaki S, Ishikawa T, Ongena G, Kuo YH, Lambein F, Murakoshi I (1991) Biosynthesis of beta-(isoxazolin-5-on-2-yl)alanine, the precursor of the neurotoxic amino acid _β-N-oxalyl-L-α β,-diaminopropionic acid. Biol Pharm Bull 39:3376–3377


Ikegami F, Itagaki S, Kamiya M, Kuo YH, Lambein F, Murakoshi I (1996) Enzymatic synthesis of two isoxazolylalanine isomers by cysteine synthases in Lathyrus species. Biol Pharm Bull 1996(19):1214–1215


Jabeen A, Khan MA, Ahmad M, Zafar M, Ahmad F (2009) Indigenous uses of economically important flora of Margallah Hills National Park, Islamabad. Pak Afr J Biotechnol 8(5):763–784


Jiang JL, Su M, Chen YR, Gao N, Jiao CJ, Sun ZX, Li FM, Wang CY (2013) Correlation of drought resistance in grass pea (Lathyrus sativus) with reactive oxygen species scavenging and osmotic adjustment. Biologia 68:231–240


Jiao C-J, Jiang J-L, Ke L-M, Cheng W, Li F-M, Li Z-X, Wang C-Y (2011) Factors a_ecting _-ODAP content in Lathyrus sativus and their possible physiological mechanisms. Food Chem Toxicol 49:543–549


Khan M, Hussain S (2014) Diversity of wild edible plants and flowering phenology of district Poonch (J&K) in the Northwest Himalaya. Indian J Sci Res 9(1):032–038


Kuo YH, Lambein F (1991) Biosynthesis of the neurotoxin _β-N-oxalyl-L-α, β-diaminopropionic acid in callus tissue of Lathyrus sativus. Phytochemistry 30:3241–3244


Kuo YH, Lambein F, Mellor L, Adlington RM (1994) Baldwin JE (1994) Ring-nitrogen of β-isoxazolinone-alanine is incorpo- rated into the neurotoxin, β-N-oxalyl-L-αβ,-diaminopropionic acid, in callus tissue of Lathyrus sativus. Phytochemistry. 37:713–715


Padmanaban G, Cheema PS, Malthi K, Lakshmanan J (1971) Sci Res 30(716):1





Lambein F, Kuo Y.-H, Kusama-Eguchi K, Ikegami F (2007) 3-N-oxalyl-L-2, 3-diaminopropanoic acid, a multifunctional plant metabolite of toxic reputation. Arkivoc 9:45–52


Lassoued S, Giosafatto CVL, Mariniello L, Neila TF (2023) Morphological characterization and in vitro digestibility of seven Lathyrus sativus (grass pea) accessions originating from Eurasia, Africa, and Canada. Eur Food Res Technol. https://doi.org/10.1007/s00217-023-04266-1


Patto MC, Vaz Rubiales D (2014) Lathyrus diversity: available resources with relevance to crop improvement L. sativus and L. cicera as cases studies. Ann of Bot 113(6):895–908


Malathi K, Padmanaban G, Rao SLN, Sarma PS (1967) Studies on the biosynthesis of β-N-oxalyl-L-α, β-diaminopropionic acid, the Lathyrus sativus neurotoxin. Biochimica et Biophysica Acta. 141:71–78


Malathi K, Padmanaban G, Sarma PS (1970) Biosynthesis of β-N-oxalyl-L-α, β-diamino-propionic acid, the Lathyrus sativus neurotoxin. Phytochemistry. 9:1603–1610


Manhas A, Kaul V (2023) Preliminary phytochemical analysis and quantification of antioxidant phenolics in commelina benghalensis using HPLC. Natl Acad Scie Lett. https://doi.org/10.1007/s40009-023-01257-4


Paissios CS, Demopoulos T (1962) Human lathyrism: a clinical and skeletal study. Clin Orthop Related Res®. 23:236–252


Quereshi MY, Pilbeam DJ, Evans CS, Bell EA (1977) The neurolathyrogen, α-amino-β-oxalylaminopropionic acid in legume seeds. Phytochemistry 16(4):477–479


Rana TS, Datt B (2008) Ethnobotanical observation among Jaunsares of Jaunsar- Bawar, Dehradun (UP). India. Int J Pharmacogn 35(5):371–374


Romano A, Giosafatto CVL, Al-Asmar A, Masi P, Romano R, Mariniello L (2019) Structure and in vitro digestibility of grass pea (Lathyrus sativus L.) flour following transglutaminase treatment. Eur Food Res Technol 245(9):1899–1905


Sharma N (2018) Studies on morphology, cytology and reproductive biology of some populations of Lathyrus aphaca L. growing in Jammu province. M.Phil dissertation submitted to University of Jammu, Jammu


Sher Z, Khan Z, Hussain F (2011) Ethnobotanical studies of some plants of Chagharzai valley district Buner Pakistan. Pak J Bot 43(3):1445–1452


Singh S, Malik RK, Balyan RS, Singh S (1995) Distribution of weed flora of wheat in Haryana. Indian J Weed Sci 27(3–4):114–121


Sokal RR, Rohlf FJ (2012) Descriptive statistics. Biometry, the principles and practice of ststistics in biological research. WH Freeman and Company, New York


Stevens CM, Bush JA (1950) New synthesis of α-amino-εguanidinon-caproic acid (homoarginine) and its possible conversion in vivo into lysine. J Biol Chem 85:91


Ullah F, Ullah A, Sohail A (2016) Medicinal and ecological diversity of weeds in wheat crop at lower Dir, Pakistan. Pak J Weed Sci Res 22(4):627–637


Van Moorhem M, Lambein F, Leybaert L (2011) Unraveling the mechanism of β- N-oxalyl-2,3 -diaminopropionic acid (β -ODAP) induced excitotoxicity and oxidative stress, relevance for neurolathyrism prevention. Food Chem Toxicol 49:550–555


Xiong J-L, Xiong Y, Bai X, Kong H, Tan R, Zhu H, Siddique K, Wang J, Turner NC (2015) Genotypic variation in the concentration of β-N-Oxalyl-L-α, β-diaminopropionic acid (β-ODAP) in Grass Pea (Lathyrus sativus L.) seeds is associated with an accumulation of leaf and pod β-ODAP during vegetative and reproductive stages at three levels of water Stress. J Agric Food Chem 63:6133–6141


Zhou GK, Kong YZ, Cui KR, Li ZX, Wang YF (2001) Hydroxyl radical scavenging activity of β-N-oxalyl-L-α, β-diaminopropionic acid. Phytochemistry 58:759–762

 


Acknowledgements


One of the authors is thankful to University of Jammu for providing financial assistance in the form of University Research Scholarship (URS). Further the author is thankful to the Head, Department of Botany, University of Jammu for providing all the necessary facilities needed to carry out this research work smoothly. The author is also thankful to CSIR-IIIM for HPLC facilities/services.


Author Information


Sharma Noopur
Department of Botany, University of Jammu, Jammu, India
nupu6sharma@gmail.com
Kaul Veenu
Department of Botany, University of Jammu, Jammu, India
veenukaul@yahoo.co.in