*Article not assigned to an issue yet
Kalaivani Sengodan, Jayanthi Somannan, Revathi Kannan, Chandrasekaran Rajamanickam
Keywords:
Anticancer, n Euphorbia hirtan , Apoptosis, MTT
Recently, the plants-derived bioactive components received a greater interests due to their significant anti-carcinogenic properties. Euphorbia hirta is a well known herbal plant, which is highlighted to show a remarkable anticancer activities against several types of cancers. In this study, we analyzed the in vitro antitumor action of E. hirta against the liver cancer HepG2 cell line. Our current findings demonstrated the existence of several bioactive metabolites in the E. hirta plant, which is studied through phyotochemical analysis and characterized through chromatogram and FT-IR analysis. The administration of E. hirta plant extract exhibited the remarkable diminution in the cell proliferation and effectively induced the apoptosis in the HepG2 cells. The outcomes of fluorescence analysis revealed that the E. hirta plant extract decreased the MMP level, and increased the accumulation of ROS and apoptosis in the liver cancer HepG2 cells. In overall, the findings of the invitro studies showed that E. hirta plant extract effectively inhibited the cell proliferation and triggered the apoptosis in the liver cancer HepG2 cells. Hence, it can be a promising source to discover the possible antitumor drugs from this plant extract to treat the liver cancer.
(*Only SPR Members can get full access. Click Here to Apply and get access)
Al-Snafi AE (2017) Pharmacology and therapeutic potential of Euphorbia hirta (Syn:Euphorbiapilulifera)—a review. J Pharm 7(3):07–20
Dabrowska C, Li M, Fan Y (2016) Apoptotic caspases in promoting cancer: implications from their roles in development and tissue homeostasis. Adv Exp Med Biol 930:89–112
Davies G (2000) Time tables of medicine. Black Dog & Leventhal, New York
Ferlay J, Soerjomataram I, Dikshit R, Eser S, Mathers C, Rebelo M (2015) Cancer incidence and mortality worldwide: sources, methods and major patterns in GLOBOCAN 2012. Int J Cancer 136:359–386
He J, Wei W, Yang Q, Wang Y (2019) Phillygenin exerts in vitro and in vivo antitumor effects in drug- resistant human esophageal cancer cells by inducing mitochondrial-mediated apoptosis, ROS generation and inhibition of the nuclear factor kappa B NF-κBsignalling pathway. Med Sci Monit 25:739–745
Kocyigit A, Guler EM (2017) Curcumin induce DNA damage and apoptosis through generation of reactive oxygen species and reducing mitochondrial membrane potential in melanoma cancer cells. Cell Mol Biol 63:97–105
Koff JL, Ramachandiran S, Bernal-Mizrachi L (2015) A time to kill: targeting apoptosis in cancer. Int J Mol Sci 16:2942–2955
Lobo V, Patil A, Phatak A, Chandra N (2010) Free radicals, antioxidants and functional foods: Impact on human health. Pharmacogn Rev 4:118–126
Nyeemet MAB, Haque MS, Akramuzzaman M, Siddika R, Sultana S, Islam R (2017) Euphorbia hirta Linn. A wonderful miracle plant of Mediterranean region: a review. J Med Plants Stud 5(3):170–175
Pham-Huy LA, He L, Pham-Huy C (2008) Free radicals, antioxidants in disease and health. Int J Biomed Sci 4:89–96
Ping KY, Darahb I, Chenc Y, Sasidharan S, Dharmara S (2013) Cytotoxicity and genotoxicity assessment of Euphorbia hirta in MCF-7 cell line model using comet assay. Asian Pac J Trop Biomed 3:692–696
Qi X, Li J, Zhou C, Lv C, Tian M (2015) MiR-142-3p suppresses SOCS6 expression and promotes cell proliferation in nasopharyngeal carcinoma. Cell Physiol Biochem 36:1743–1752
Rahman M, Islam B, Biswas M, Alam AHMK (2015) In vitro antioxidant and free radical scavenging activity of different parts of Tabebuia pallida growing in Bangladesh. BMC Res Notes 8:621
Sharma N, Samarakoon KW, Gyawali R, Park YH, Lee SJ, Oh SJ, Lee TH, Jeong DK (2014) Evaluation of the antioxidant, anti-inflammatory, and anticancer activities of Euphorbia hirta ethanolic extract. Molecules 19:14567–14581
Shih MF, Cherng JY (2012) Potential applications of Euphorbia hirta in pharmacology. Drug Discov Res Pharmacogn 8:166–180
Shrivastava S, Nagi R, Sharma S, Sanadi SAR, Dafallah ET, Ragit A (2020) Molecular targeted therapy: novel therapeutic approach for head and neck cancer. Ther Deliv 11(10):637–651
Sidambaram RR, Dinesh MG, Jayalakshmi ET (2011) An in vitro study of cytotoxic activity of Euphorbia hirta on hep2 cells of human epithelioma of larynx. Int J Pharm Sci 3:101–103
Singh AK, Kumar R, Pandey AK (2018) Hepatocellular carcinoma: causes, mechanism of progression and biomarkers. Curr Chem Genom Transl Med 12:9–26
Sofowora A, Ogunbodede E, Onayade A (2013) The role and place of medicinal plants in the strategies for disease prevention. Afr J Tradit Complement Altern Med 10(5):210–229
Subramanian SP, Bhuvaneshwari S, Prasath GS (2011) Antidiabetic and antioxidant potentials of Euphorbia hirta leaves extract studied in streptozotocin-induced experimental diabetes in rats. Gen Physiol Biophys 30(3):278–285
Thomford NE, Senthebane DA, Rowe A, Munro D, Seele P, Maroyi A, Dzobo K (2018) Natural products for drug discovery in the 21st century: innovations for novel drug discovery. Int J Mol Sci 19(6):1578
Tiwari N, Mishra A, Bhatt G, Chaudhary A (2015) Anti-stress activity of a bioflavanoid: quercetin from Euphorbia hirta. Br J Pharm Res 6(2):68–75
Tripathi SK, Rengasamy KRR, Biswal BK (2020) Plumbagin engenders apoptosis in lung cancer cells via caspase-9 activation and targeting mitochondrial-mediated ROS induction. Arch Pharm Res 43:242–256
Tuhin RH, Begum MM, Rahman MS, Karim R, Begum T, Ahmed SU, Mostofa R, Hossain A, Abdel-Daim M, Begum R (2017) Wound healing effect of Euphorbia hirta linn. (Euphorbiaceae) in alloxan induced diabetic rats. BMC Complement Altern Med 17(1):423
Yang JD, Roberts LR (2010) Hepatocellular carcinoma: a global view. Nat Rev Gastroenterol Hepatol 7:448–458
Yang L, Hong S, Wang Y, Chen H, Liang S, Peng P, Chen Y (2015) Development and external validation of nomograms for predicting survival in nasopharyngeal carcinoma patients after definitive radiotherapy. Sci Rep 5:15638
Yuan H, Ma Q, Piao G (2016) The traditional medicine and modern medicine from natural products. Molecules 21(5):559
Zhang SX, Qiu QH, Chen WB, Liang CH, Huang B (2014) Celecoxib enhances radiosensitivity via induction of G(2)-M phase arrest and apoptosis in nasopharyngeal carcinoma. Cell Physiol Biochem 33:1484–1497
Zhang W, Zhang Q, Jiang Y, Li F, Xin H (2016) Effects of ophiopogonin B on the proliferation and apoptosis of SGC-7901 human gastric cancer cells. Mol Med Rep 13:4981–4986
The authors would like to thank Shri Sakthikailassh Women’s College for their kind support for all lab studies.