Optimization of the process for obtaining Morinda royoc crude extract bioactive against phythopathogens

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Online ISSN : 2229-4473.
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Doi: 10.1007/s42535-023-00675-5
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Keywords: Anthraquinones, Antifungal activity, Extraction process


Abstract


Currently, there is an increased search for alternatives to reduce the excessive use of synthetic pesticides in control of pests and diseases in plants. Use of natural plant products is considered an efficient way to reduce the use of these harmful products and achieve the control of phytopathogens in agricultural production. In this sense, Morinda royoc L. is known for the high content of anthraquinones (AQs) in its roots, which have been studied for their distinct bioactivity. Therefore, the aim of this study was to optimize the extraction procedure to obtain crude extracts of M. royoc roots rich in bioactive AQs and to evaluate the antifungal activity in vitro against the fungi Botrytis cinerea, Neofabraea alba and Venturia inaequalis. The extraction procedure by maceration was optimized in terms of extraction time, hydroethanolic mixture and temperature parameters; in vitro inhibitory effect of the obtained extract on mycelial growth and conidial germination of three fungi was evaluated. M. royoc root extract was optimized at 42.31 mg AQs/g dry weight; and its antifungal potential was demonstrated by complete inhibition of conidial germination of B. cinerea, N. alba, and V. inaequalis at concentrations of 4.8 to 2.4 mg/ml; 4.8 to 0.15 mg/ml; and 4.8 to 0.3 mg/ml, respectively. Based on the efficacy of the extract as an in vitro antifungal control, it could be used as a promising product for crop protection.


Anthraquinones, Antifungal activity, Extraction process


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Acknowledgements


The authors thank Dr. Stefan Kunz and Bio-Protect GmbH of Germany for their professional services in the development of this research. In addition, the authors would like to thank the German Federal Ministry of Education and Research for providing the funds that supported the research under the project “BIOPESTIZIDE”. Authors are also grateful to colleagues from the Institute Kompetenzzentrum Obstbau Bodensee (KOB), especially to Dr. Daniel Alexandre Neuwald, head of the Postharvest Physiology and Technology Department; for his help and support.


Author Information


Linares Claudia
Natural Products Department, Centro de Bioplantas, Universidad de Ciego de Ávila Máximo Gómez Báez, Ciego de Ávila, Cuba

Cid Geeisy A.
Natural Products Department, Centro de Bioplantas, Universidad de Ciego de Ávila Máximo Gómez Báez, Ciego de Ávila, Cuba