Vegetos
(An International Journal of Plant Research & Biotechnology)
Molecular characterization of Psidium guajava genotypes using microsatellite markers
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Research Articles | Published: 30 April, 2025
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Keywords: Genetic diversity, Germplasm, The common guava, Polymorphism, SSR
Abstract
A set of twenty-four Psidium guajava genotypes including exotic cultivar had been genetically characterized using a total of twenty microsatellite (SSR) markers spanning a total of 11 chromosomes. The average number of alleles per locus was 2.93. Fifteen markers (out of the twenty used) were highly polymorphic and could discriminate local cultivars and exotic collection. Two markers, mPgCIR352 and mPgCIR175, showed the highest level of polymorphism, whereas marker mPgCIR352 showed the maximum marker index for high level of resolving capacity amongst all markers tested. Marker mPgCIR321, with 0.778 PIC value present on linkage group 11, has been identified to be associated with fruit yield. Molecular variance study resulted at 92% genetic variance within population and 8% between population. First three axes explained 53.32% variation through principal coordinate analysis. This study explains variation among the existing popular cultivar, local cultivar, and few exotic cultivars which can be helpful in designing cross breeding programmes through the selection of the superior parental varieties.
References
Arévalo-Marín E, Casas A, Alvarado-Sizzo H, Ruiz-Sanchez E, Castellanos-Morales G, Jardón-Barbolla L, Fermin G, Padilla-Ramírez JS, Clement CR (2024) Genetic analyses and dispersal patterns unveil the Amazonian origin of guava domestication. Sci Rep 14(1):15755
Briceno A, Aranguren Y, Fermin G (2010) Assessment of guava-derived SSR markers for the molecular characterization of Myrtaceae from different ecosystems in Venezuela. Acta Hort 849:139–146
Charles WW, Philip ES, Carl WC (2006) Determination of organic acids and sugars in Guajava L. cultivars by high-performance liquid chromatography. J Food Agri 33:777–780
Chen TW, Chang-Chai NG, Wang CY, Shyu YT (2007) Molecular identification and analysis of Psidium guajava L. from Indigenous tribes of Taiwan. J Food Drug Anal 15:82–88
Christenhusz MJM, Byng JW (2016) The number of known plants species in the world and its annual increase. Phytotaxa Magnolia Press 261(3):201–217
Cordeiro GM, Pan YB, Henry RJ (2003) Sugarcane microsatellites for the assessment of genetic diversity in sugarcane germplasm. Plant Sci 165:181–189
Dinesh MR, Vasugi C (2010) Guava improvement in India and future needs. J Horti Sci 5(2):94–108
Dos Santos JM, Duarte Filho LSC, Soriano ML, da Silva PP, Nascimento VX, de Souza Barbosa GV, Todaro AR, Ramalho Neto CE, Almeida C (2012) Genetic diversity of the main progenitors of sugarcane from the RIDESA germplasm bank using SSR markers. Ind Crop Prod 40:145–150
Essa MM, Vijayan RK, Al-Rawahi AS, Guillemin GJ, Selvaraju S, Memon MA (2013) Guava and potential health benefits. Food as medicine. Nova Science Publishers, Inc, pp 271–290
Feria-Romero IA, Astudillo-Dela HV, Chavez-Soto MA, Rivera-Arce E, Lopez M, Serrano H, Lozoya X (2009) RAPD markers associated with Quercetin accumulation in Psidium guajava. Biol Plant 53:125–128
Govaerts R, Sobral M, Ashton P, Barrie F, Holst BK, Landrum LL, Matsumoto K, Mazine FF, Nic Lughadha E, Proenca C, Soares-Silva LH, Wilson PG, Lucas E (2008) World Checklist of Myrtaceae. Kew Publishing, London: Board of Trustees of the Royal Botanic Gardens, Kew 455
Kherwar D, Usha K, Amitha Mithra SV, Singh B (2018) Microsatellite (SSR) marker assisted assessment of population structure and genetic diversity for morpho-physiological traits in guava (Psidium Guajava L). J Plant Biochem Biotech 27(3):284–292
Kanupriya MLP, Aswath C, Reddy L, Padmakar B, Vasugi C, Dinesh MR (2011) Cultivar identification and genetic finger printing of guava (Psidium guajava L.) using microsatellite markers. Int J Fruit Sci 11:184–196
Mahattanatawee K, Manthey JA, Luzio G, Talcott ST, Goodiner K, Baldwin EA (2006) Total antioxidant activity and fibre content of select Florida-grown tropical fruits. J Agric Food Chem 54(19):7355–7363
Mathiazhagan M, Elangovan D, Chinnaiyan V, Shivashankara KS, Sudhakar Rao DV, Ravishankar KV (2024) A high-density linkage map construction in guava (Psidium Guajava L.) using genotyping by sequencing and identification of QTLs for leaf, Peel, and pulp color in an intervarietal mapping population. Front Plant Sci 15:1335715. https://doi.org/10.3389/fpls.2024.1335715
Mehmood A, Luo S, Ahmad N, Dong C, Mahmood T, Sajjad Y, Jaskani M, Sharp P (2015) Molecular variability and phylogenetic relationships of guava (Psidium Guajava L.) cultivars using inter-primer binding site (iPBS) and microsatellite (SSR) markers. Genetic Resources and Crop Evolution, p 63
Naga Chaithanya MV, Dinesh MR, Vasugi C, Reddy DC, Sailaja D, Aswath C (2014) Assessment of genetic diversity in guava (Psidium Guajava L) germplasm using microsatellites. J Hort Sci 9(2):117–125
Pandey P, Kumar R, Kumar R, Mishra DS, Jena AS, Kumar J (2017) Morphological and molecular characterization of guava. Int J Chem Stud 5(4):533–538
Risterucci AM, Duval MF, Rohde W, Billotte N (2005) Isolation and characterization of microsatellite loci from Psidium guajava L. Mol Ecol Notes 5:745–748
Ritter NN, Rodríguez-Medina, Velásquez B, Rivero D, Rodríguez JA, Martínez F, Valdés-Infant J (2010) QTL (Quantitative trait Loci) analysis in guava. Acta Hort 849:193–202
Rodriguez N, Valdes-Infante J, Becker D, Velazquez B, Gonzalez G, Sourd D (2007) Characterization of guava accessions by SSR markers, extension of the molecular linkage map, and mapping of QTLs for vegetative and reproductive characters. Acta Hort 735:201–215
Rodríguez NN, Valdés J, Rodríguez JA, Velásquez JB, Rivero D, Martínez F, Gonzalez G, Sourd DG, Gonzalez L, Cañizares J (2010) Genetic resources and breeding of guava (Psidium Guajava L.) in Cuba. Biotecnología Aplicada 27(3):238–240
Setiawan B, Sulaeman A, Giraud DW, Driskell JA (2001) Carotenoid content of selected Indonesian fruits. J Food Compos Anal 14:169–176
Semwal DP, Longkumar S, Chandra P, Rathi RS, Rai KM, Arya M, Ahlawat SP, Singh PK (2024) Diversity distribution analysis of guava (Psidium Guajava L.) populations in cultivated and wild habitats in the Mid-Hills of Uttarakhand, India. Agriculture 14:575. https://doi.org/10.3390/agriculture14040575
Sharma N, Dubey AK, Srivastav M, Singh BP, Singh AK, Singh NK (2015) Assessment of genetic diversity in grapefruit (Citrus paradisi Macf) cultivars using physicochemical parameters and microsatellite markers. AJCS 9:62–68
Sharma N, Kherwar D, Ajay KM, Singh B, Usha K (2013) Molecular breeding to improve guava (Psidium Guajava L.) current status and future prospective. Sci Horti 164:578–588
Sözen E (2010) Evaluation of Issr markers to assess genetic variability and relationship among winter Triticale (X Triticosecale Wittmack) cultivars. Pakistan J Bot 42(4):2755–2763
Valdes-Infante J, Rodriguez MNN, Velasquez B, Rivero D, Martinez F, Risterucci AM, Billotte N, Becker D, Ritter E, Rohde W (2010) Comparison of the polymorphism level discriminating capacity and informativeness of morpho agronomic traits and molecular markers in guava (Psidium Guajava L). Acta Hort 849:121–132
Verma VK, Rymbai H, Deshmukh NA, Bhattacharjee B, Jha AK, Patel RK, Mawlein J, Deka BC, Hazarika S, Mishra VK (2024) Genetic diversity and stability analysis of the improved cultivars of guava and their related species. Sci Hort 333:113260
Viji S, Zhang D, Harris DL, Yadav AK, Zee FT, Meinhardt LW, Dhekney SA (2014) Genetic characterization of guava (Psidium Guajava L.) germplasm in the united States using microsatellite markers. Genet Resour Crop Evol 61:829–839. https://doi.org/10.1007/s10722-014-0078-5
Pardo E, Hernandez K, Cvadia T (2023) Molecular characterization of guava Psidium guajava in Cerete, Cordoba, Colombia. Notulae botanicae horti agrobotanici Cluj-Napoca. 51(3). https://doi.org/10.15835/nbha51313305
Medeiros FLB, Santos CAF, Costa AES (2021) In silico microsatellite transferability from Psidium guajava to Eucalyptus globulus validated by PCR. Genet. Mol. Res 20(4):gmr18985,https://doi.org/10.4238/gmr18985
Kumari S, Arumugam N, Singh R, Srivastav M, Banoth S, Mithra AC, Arun MB, Goswami AK, Khan YJ (2023) Diversity analysis of guava (Psidium guajava) germplasm collection. Indian J Agricultural Sci 88(3):489–497. https://doi.org/10.56093/ijas.v88i3.78740
Kumar C, Kumar R, Singh SK, Goswami AK, Nagaraja A, Paliwal R et al (2020) Development of novel g-SSR markers in guava (Psidium Guajava L.) Cv. Allahabad Safeda and their application in genetic diversity, population structure and cross species transferability studies. PLoS ONE 15(8):e0237538. https://doi.org/10.1371/journal.pone.0237538
Oliveira JAVS, Santos EA, Viana AP, Walter FHB, Ribeiro RM (2022) Genetic-molecular characterization in guava full-sib progeny. Bragantia 81:e3322. https://doi.org/10.1590/1678-4499.20210267
de Oliveira Bernardes C, Tuler AC, Canal D, Carvalho MS, Ferreira A, da Silva Ferreira MF (2022) Genetic diversity and population structure of Psidium species from restinga: A coastal and disturbed ecosystem of the Brazilian Atlantic forest. Biochem Genet 60(6):2503–2514. https://doi.org/10.1007/s10528-022-10222-7
Chiveu JC, Mueller M, Krutovsky KV, Kehlenbeck K, Pawelzik E, Naumann M, Akademgorodok KR (2019) Genetic diversity of common guava in Kenya: an underutilized naturalized fruit species. Fruits
Author Information
Department of Agriculture, Swami Vivekananda University, West Bengal, India