Limitations to photosynthesis during progressive exposure of acai seedlings to high temperature stress

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DOI: 10.1007/s42535-026-01659-x
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Keywords: n Euterpe oleracean , Heat stress, Lipid peroxidation, Photo-oxidative damages, Photorespiration


Abstract


This study was performed to examine some physiological responses related to net photosynthesis in acai (Euterpe oleracea Mart.) seedlings to high temperature (HT) stress. The seedlings were incubated at 28 °C (Control) and at 40 °C (HT-stress treatment) for 1, 6 and 11 days. Relative to control, the HT-stressed seedlings showed 36%, 81% and 87% decreases in A on days 1, 6 and 11. The stomatal conductance and transpiration decreased by 80% regardless of stress duration, while substomatal CO2 concentration increased over time until reach the highest average on day 11. The relative water content in HT-stressed seedlings was decreased by 31% on days 1 and 6 and by 85% on day 11. Significant decrease in the maximum quantum efficiency of photosystem (PS) II photochemistry was only observed on day 11, but actual quantum yield of PSII electron transport, photochemical and non-photochemical quenching and Rubisco-initial activity were progressively decreased over the stress period. Relative to control, glycolate oxidase activity in HT-stressed plants was only increased (59%) on day 11. The HT-stress induced 37% and 66% decreases in sucrose and starch contents regardless of stress period and induced 22% (days 1 and 6) and 46% (day 11) decreases in glucose. Lipid peroxidation in HT-stressed plants was evident on days 1 and 11. Therefore, net photosynthesis was strongly constrained by reduced Rubisco carboxylase activity and enhanced photorespiration, highlighting the sensitivity of acai seedlings to HT stress.

n                     Euterpe oleracean                  , Heat stress, Lipid peroxidation, Photo-oxidative damages, Photorespiration


References


Atkin OK, Tjoelker MG (2003) Thermal acclimation and the dynamic response of plant respiration to temperature. Trends Plant Sci. https://doi.org/10.1016/S1360-1385(03)00136-5


Balfagón D, Zandalinas SI, Baliño P et al (2018) Involvement of ascorbate peroxidase and heat shock proteins on citrus tolerance to combined conditions of drought and high temperatures. Plant Physiol Biochem. https://doi.org/10.1016/j.plaphy.2018.03.029


Booker FL, Reid CD, Brunschon-Harti S et al (1997) Photosynthesis and photorespiration in soybean [Glycine max (L.) Merr.] chronically exposed to elevated carbon dioxide and ozone. J Exp Bot. https://doi.org/10.1093/jxb/48.10.1843


Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein–dye binding. Anal Biochem. https://doi.org/10.1016/0003-2697(76)90527-3


Cakmak I, Horst WJ (1991) Effect of aluminium on lipid peroxidation, superoxide dismutase, catalase, and peroxidase activities in root tips of soybean (Glycine max). Physiol Plant. https://doi.org/10.1111/j.1399-3054.1991.tb00121.x


Carvalho LC, Gonçalves EF, Silva JM et al (2021) Potential phenotyping methodologies to assess inter- and intravarietal variability and to select grapevine genotypes tolerant to abiotic stress. Front Plant Sci. https://doi.org/10.3389/fpls.2021.718202


Chen WR, Zheng JS, Li YQ et al (2012) Effects of high temperature on photosynthesis, chlorophyll fluorescence, chloroplast ultrastructure, and antioxidant activities in fingered citron. Russ J Plant Physiol. https://doi.org/10.1134/S1021443712060040


Duan H, Amthor JS, Duursma RA et al (2013) Carbon dynamics of eucalypt seedlings exposed to progressive drought in elevated [CO2] and elevated temperature. Tree Physiol. https://doi.org/10.1093/treephys/tpt061


Dusenge ME, Duarte AG, Way DA (2019) Plant carbon metabolism and climate change: elevated CO2 and temperature impacts on photosynthesis, photorespiration and respiration. New Phytol. https://doi.org/10.1111/nph.15283


Feng B, Liu P, Li G et al (2014) Effect of heat stress on the photosynthetic characteristics in flag leaves at the grain-filling stage of different heat-resistant winter wheat varieties. J Agro Crop Sci. https://doi.org/10.1111/jac.12045


Flexas J, Barbour MM, Brendel O et al (2012) Mesophyll diffusion conductance to CO2: an unappreciated central player in photosynthesis. Plant Sci. https://doi.org/10.1016/j.plantsci.2012.05.009


Gao Y-B, Zheng W-W, Zhang C et al (2019) High temperature and high light intensity induced photoinhibition of bayberry (Myrica rubra Sieb. et Zucc.) by disruption of D1 turnover in photosystem II. Sci Hort. https://doi.org/10.1016/j.scienta.2019.01.007


Geigenberger P, Stitt M (1993) Sucrose synthase catalyzes a readily reversible reaction in developing potato tubers and other plant tissues. Planta. https://doi.org/10.1007/BF00194429


Genty B, Briantais J-M, Baker NR (1989) The relationship between the quantum yield of photosynthetic electron transport and quenching of chlorophyll fluorescence. Biochim Biophys Acta. https://doi.org/10.1016/S0304-4165(89)80016-9


González L, González-Vilar M (2001) Determination of relative water content. In: Roger MJR (ed) Handbook of plant ecophysiology techniques. Kluwer Academic Publishers, Dordrecht


Guo Y-P, Zhou H-F et al (2006) Photosynthetic characteristics and protective mechanisms against photooxidation during high temperature stress in two citrus species. Sci Hort. https://doi.org/10.1016/j.scienta.2006.01.029


Hao L, Guo L, Li R et al (2019) Responses of photosynthesis to high temperature stress associated with changes in leaf structure and biochemistry of blueberry (Vaccinium corymbosum L.). Sci Hort. https://doi.org/10.1016/j.scienta.2018.11.007


Hasanuzzaman M, Bhuyan MHMB, Parvin K et al (2020) Regulation of ROS metabolism in plants under environmental stress: a review of recent experimental evidence. Int J Mol Sci. https://doi.org/10.3390/ijms21228695


Hou W, Sun AH, Chen HL et al (2016) Effects of chilling and high temperatures on photosynthesis and chlorophyll fluorescence in leaves of watermelon seedlings. Biol Plant. https://doi.org/10.1007/s10535-015-0575-1


Hsie BS, Mendes KR, Antunes WC et al (2015) Jatropha curcas L. (Euphorbiaceae) modulates stomatal traits in response to leaf-to-air vapor pressure deficit. Biomass Bioenerg. https://doi.org/10.1016/j.biombioe.2015.07.014


Huang B, Xu Q (2000) Root growth and nutrient element status of creeping bentgrass cultivars differing in heat tolerance as influenced by supraoptimal shoot and root temperatures. J Plant Nutr. https://doi.org/10.1080/01904160009382075


Jin S-H, Li X-Q, Zheng B-S et al (2010) Response of the photosynthesis and antioxidant systems to high temperature stress in Euonymus japonicus seedlings. Forest Sci. https://doi.org/10.1093/forestscience/56.2.172


Kohila S, Gomathi R (2018) Adaptive physiological and biochemical response of sugarcane genotypes to high temperature stress. Indian J Plant Physiol. https://doi.org/10.1007/s40502-018-0363-y


Kumar S, Gupta D, Nayyar H (2012) Comparative response of maize and rice genotypes to heat stress: status of oxidative stress and antioxidants. Acta Physiol Plant. https://doi.org/10.1007/s11738-011-0806-9


Lima ALS, Da Matta FM, Pinheiro HA et al (2002) Photochemical responses and oxidative stress in two clones of Coffea canephora under water deficit conditions. Environ Exp Bot. https://doi.org/10.1016/S0098-8472(01)00130-7


Marias DE, Meinzer FC, Still C (2017) Impacts of leaf age and heat stress duration on photosynthetic gas exchange and foliar nonstructural carbohydrates in Coffea arabica. Ecol Evol. https://doi.org/10.1002/ece3.2681


Martins SCV, Galmés J, Cavatte PC et al (2014) Understanding the low photosynthetic rates of sun and shade coffee leaves: bridging the gap on the relative roles of hydraulic, diffusive and biochemical constraints to photosynthesis. PLoS ONE. https://doi.org/10.1371/journal.pone.0095571


Maxwell K, Johnson GN (2000) Chlorophyll fluorescence—a practical guide. J Exp Bot. https://doi.org/10.1093/jexbot/51.345.659


McAdam SA, Brodribb TJ (2015) The evolution of mechanisms driving the stomatal response to vapor pressure deficit. Plant Physiol. https://doi.org/10.1104/pp.114.252940


McAdam SA, Sussmilch FC, Brodribb TJ (2016) Stomatal responses to vapour pressure deficit are regulated by high speed gene expression in angiosperms. Plant Cell Environ. https://doi.org/10.1111/pce.12633


Nardini A, Õunapuu-Pikas E, Savi T (2014) When smaller is better: leaf hydraulic conductance and drought vulnerability correlate to leaf size and venation density across four Coffea arabica genotypes. Funct Plant Biol. https://doi.org/10.1071/fp13302


Neves LH, Santos RIN, Teixeira et al (2019) Leaf gas exchange, photochemical responses and oxidative damages in assai (Euterpe oleracea Mart.) seedlings subjected to high temperature stress. Sci Hort. https://doi.org/10.1016/j.scienta.2019.108733


Oliveira HO, Castro GLS, Correa LO et al (2019) Coupling physiological analysis with proteomic profile to understand the photosynthetic responses of young Euterpe oleracea palms to drought. Photosyn Res. https://doi.org/10.1007/s11120-018-0597-6


Perdomo JA, Capó-Bauçà S, Carmo-Silva E et al (2017) Rubisco and Rubisco activase play an important role in the biochemical limitations of photosynthesis in rice, wheat, and maize under high temperature and water deficit. Front Plant Sci. https://doi.org/10.3389/fpls.2017.00490


Portillo-Estrada M (2024) Limitations of plant stress tolerance upon heat and CO2 exposure in black poplar: assessment of photosynthetic traits and stress volatile emissions. Plants. https://doi.org/10.3390/plants13081165


Pregitzer KS, King JS, Burton AJ et al (2000) Responses of tree fine roots to temperature. New Phytol. https://doi.org/10.1046/j.1469-8137.2000.00689.x


R Core Team, v. 4.1.0 (2021) A Language and Environment for Statistical Computing. R Foundation for Statistical Computing, Vienna, Austria [ISBN3-900051-07-0]. https://www.r-project.org/. https://www.r-project.org/


Samat AT, Soltabayeva A, Bekturova A et al (2025) Plant responses to heat stress and advances in mitigation strategies. Front Plant Sci. https://doi.org/10.3389/fpls.2025.1638213


Sarkar J, Chakraborty B, Chakraborty U (2016) Temperature stress induced antioxidative and biochemical changes in wheat (Triticum aestivum L.) cultivars. J Plant Stress Physiol. https://doi.org/10.19071/jpsp.2016.v2.3076


Siddiqui MH, Al-Khaishany MY, Al-Qutami MA et al (2015) Morphological and physiological characterization of different genotypes of faba bean under heat stress. Saudi J Biol Sci. https://doi.org/10.1016/j.sjbs.2015.06.002


Silva PA, Cosme VS, Rodrigues KCB et al (2017) Drought tolerance in two oil palm hybrids as related to adjustments in carbon metabolism and vegetative growth. Acta Physiol Plant. https://doi.org/10.1007/s11738-017-2354-4


Silvestre WVD, Pinheiro HA, Souza RORM et al (2016) Morphological and physiological responses of açaí seedlings subjected to different watering regimes. Rev Bras Eng Agric Ambient. https://doi.org/10.1590/1807-1929/agriambi.v20n4p364-371


Stitt M, Lilley RMCC, Gerhardt R et al (1989) Metabolite levels in specific cells and subcellular compartments of plant leaves. Methods Enzymol. https://doi.org/10.1016/0076-6879(89)74035-0


Sulpice R, Tschoep H, Von Korff M et al (2007) Description and applications of a rapid and sensitive non-radioactive microplate-based assay for maximum and initial activity of D- ribulose-1,5-bisphosphate carboxylase/oxygenase. Plant Cell Environ. https://doi.org/10.1111/j.1365-3040.2007.01679.x


Trethewey RN, Geigenberger P, Riedel K et al (1998) Combined expression of glucokinase and invertase in potato tubers leads to a dramatic reduction in starch accumulation and a stimulation of glycolysis. Plant J. https://doi.org/10.1046/j.1365-313X.1998.00190.x


Wang Q–L, Chen J–H, He N–Y et al (2018) Metabolic reprogramming in chloroplasts under heat stress in plants. Int J Mol Sci. https://doi.org/10.3390/ijms19030849


Yin Y, Li S, Liao W et al (2010) Photosystem II photochemistry, photoinhibition, and the xanthophyll cycle in heat-stressed rice leaves. J Plant Physiol. https://doi.org/10.1016/j.jplph.2009.12.021


Zahra N, Hafeez MB, Ghaffar A et al (2023) Plant photosynthesis under heat stress: effects and management. Environ Exp Bot. https://doi.org/10.1016/j.envexpbot.2022.105178


Zha Q, Xi X, Jiang A et al (2016) Changes in the protective mechanism of photosystem II and molecular regulation in response to high temperature stress in grapevines. Plant Physiol Biochem. https://doi.org/10.1016/j.plaphy.2016.01.024


BakerNR (2008) Chlorophyll fluorescence: a probe of photosynthesis in vivo. Annual Rev. Plant Biol. 59:89–113. https://doi.org/10.1146/annurev.arplant.59.032607.092759

 


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Instituto Socioambiental e dos Recursos Hídricos, Universidade Federal Rural da Amazônia, Belém, Brazil