Isolation and characterization of l-asparaginase from seaweeds collected from Rameswaram coast, Southeast India

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Print ISSN : 0970-4078.
Online ISSN : 2229-4473.
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Doi: 10.1007/s42535-023-00648-8
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Keywords: n l-Asparaginase, n Ulva intestinalis (green), n Lobophora variegate (brown), n Halymenia dilatata (red), HPLC


Abstract


The present study evaluates the production of l-asparaginase enzyme from the seaweeds of Ulva intestinalis L. (green), Lobophora variegata L. (brown), and Halymenia dilatata L. (red). The concentrations of ammonia and protein are tested in all three seaweeds. Among them, Ulva intestinalis contained a higher concentration of ammonia and protein with values of 404.166 ± 33.815 mg/mL and 8.233 ± 0.109 mg/mL, respectively. The HPLC results indicated that Lobophora variegata showed a higher peak (5) area percentage with a value of 35.911%, followed by Halymenia dilatata, which showed a peak (5) area percentage of 35.406%. Finally, Ulva intestinalis showed a peak (5) area percentage of 32.063. However, the HPLC results of the  l-asparaginase enzyme (extracted from the seaweeds) showed the highest peak (1) area percentage with a value of 100%. These findings show that the l-asparaginase enzyme extracted from these seaweeds might be utilized in food processing and pharmacological medicines. Further studies are needed to explore the biological activity of the l-asparaginase enzyme extracted from these seaweeds.



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Acknowledgements


The authors thank the authorities of the Bharathidasan University for providing access to the facilities and for their encouragement. AA thanks the Adi Dravider Welfare Department, Govt. of Tamil Nadu for its financial support.


Author Information


Sivakumar S. R.
Department of Botany, Bharathidasan University, Tiruchirappalli, India
drsrsivakumar@gmail.com
Annamalai Aravinth
Department of Marine Science, Bharathidasan University, Tiruchirappalli, India


Ramasamy Manikandan
Department of Zoology, Periyar Arts College, Cuddalore, India


Sarbudeen M.
Department of Botany, Bharathidasan University, Tiruchirappalli, India