In vitro response of gamma irradiated Vanilla planifolia jacks. Shoots in the face of water stress

*Article not assigned to an issue yet

, ,


Research Articles | Published:

Print ISSN : 0970-4078.
Online ISSN : 2229-4473.
Website:www.vegetosindia.org
Pub Email: contact@vegetosindia.org
Doi: 10.1007/s42535-024-00850-2
First Page: 0
Last Page: 0
Views: 1007


Keywords: Gamma irradiation, In vitro selection, Mutagenesis, Osmolytes, Polyethylene glycol


Abstract


Currently, premature fruit fall is one of the major problems in vanilla (Vanilla planifolia Jacks.) cultivation. This phenomenon has been related to its high susceptibility to drought, a consequence of the low genetic variability of this crop. For this reason, it is important to undertake genetic improvement programs to obtain genotypes with greater tolerance to drought. With this aim, the present work irradiated cluster shoots with different doses (0, 0.5, 1, 3, 5, 7, 9, 11, 13, 15, 17, and 19 Gy) of gamma rays. Subsequently, choose shoots of doses 0, 3, 7 and 13 Gy to subject them to in vitro selection to drought cultivate them in Murashige & Skoog (MS) medium, adding 0, 10, and 15% polyethylene glycol (PEG). The results showed a greater proliferation of irradiated shoots with doses of 0.5 and 1 Gy (8.88 ± 3.04 and 6.43 ± 0.98, respectively). Shoots irradiated with 0.5 Gy had faster growth (19.26 ± 6.87 mm), while those irradiated with 3 Gy showed a higher number of leaves (2.38 ± 0.71). The cluster shoots irradiated with 9, 15, 17, and 19 Gy lost their ability to multiply, unlike the shoots subjected to 13 Gy, which retained their capacity for vegetative reproduction. The best response to in vitro selection to drought was obtained with the dose of 13 Gy. These results highlight the usefulness of combining gamma radiation work with in vitro selection by PEG to obtain lines with tolerance to water deficits for use in improvement programs for this valuable crop.


Gamma irradiation, In vitro selection, Mutagenesis, Osmolytes, Polyethylene glycol


*Get Access

(*Only SPR Members can get full access. Click Here to Apply and get access)

Advertisement

References


Akte J, Yasmin S, Bhuiyan MJH, Khatun F, Roy J, Goswami K (2016) In vitro screening of rice genotypes using PEG under drought stress. Progressive Agric 27:128–135. https://doi.org/10.3329/pa.v27i2.29321


Barrera RA, Herrera CB, Jaramillo VJ, Escobedo GJ, Bustamante GA (2009) Caracterización De Los sistemas de producción de vainilla (Vanilla planifolia A.) bajo naranjo y en malla sombra en El Totonacapan. Trop Subtrop Agroecosystems 10:199–212


Bates LS, Waldren RP, Teare ID (1973) Rapid determination of free proline for water stress studies. Plant Soil 39:205–208. https://doi.org/10.1007/BF00018060


Chandran S, Puthur J (2009) Assorted response of mutated variants of Vanilla planifolia Andr. Towards drought. Acta Physiol Plant 31:1023–1029. https://doi.org/10.1007/s11738-009-0321-4


Diez MC, Moreno F, Gantiva EA (2017) Effects of light intensity on the morphology and CAM photosynthesis of Vanilla planifolia Andrews. Rev Fac Nac Agron Medellin 70:8023–8033. https://doi.org/10.15446/rfnav70n1.61736


Esnault MA, Legue F, Chenal C (2010) Ionizing radiation: advances in plant response. Environ Exp Bot 68. 231.237


Gantiva EA, Díez MC, Moreno F (2020) Efecto de la interacción luz-agua sobre la fotosíntesis de la Vanilla planifolia (Orchidaceae). Rev Biol Trop 68:1250. https://doi.org/10.15517/RBT.V68I4.41385


Grieve CM, Grattan SR (1983) Rapid assay for determination of water-soluble quaternary ammonium compounds. Plant Soil 70:303–307. https://doi.org/10.1007/BF02374789


Hernández-Pérez CA, Gómez-Merino FC, Spinoso-Castillo JL, Bello-Bello JJ (2021) In vitro screening of sugarcane cultivars (Saccharum Spp. Hybrids) for tolerance to Polyethylene Glycol-Induced Water stress. Agron 11:598. https://doi.org/10.3390/agronomy11030598


Hernández J, Curtí S, Ríos A (2019) Retención De frutos en Vanilla planifolia jacks. Ex Andrews como reguladores de crecimiento. Agron Mesoam 30:695–704. https://doi.org/10.15517/am.v30i3.33988


Jan S, Parween T, Siddiqi TO, Mahmooduzzafar (2012) Effect of gamma radiation on morphological, biochemical, and physiological aspects of plants and plant products. Environ Rev 20:17–39. https://doi.org/10.1139/a11-021


Kacem NS, Delporte F, Muhovski Y, Djekoun A, Watillon (2017) In vitro screening of durum wheat against water-stress mediated through polyethylene glycol. JGEB 15:239–247. https://doi.org/10.1016/j.jgeb.2017.04.004


Kim JH, Chung BY, Kim JS, Wi SG (2005) Effects of gamma-irradiation in plants gamma-irradiation on growth, photosynthesis, and antioxidative capacity of red pepper (Capsicum annuum L.) plants. J Plant Biol 48:47–56. https://doi.org/10.1007/BF03030564


Laisina JKJ, Maharijaya A, Sobir, Purwito A (2021) In vitro screening of drought tolerant potatoes (Solanum tuberosum L.) of IPB collections. Indonesian J Agricultural Sci 26:235–242. https://doi.org/10.13057/biodiv/d220204


Lestari EG, Sukmadjaja D, Mariska DI (2006) Perbaikan Ketahanan tanaman panili terhadap penyakit layu melalui kultur in vitro. J Litbang 25:149–153


Ma’Arup R, Ali NS, Ahmad F, Ahmad Z, Mohamed Norawi MF, Moinuddin HF (2022) Effects of gamma irradiation on morphology and protein differential in M1V1 population of Vanilla planifolia Andrews. Int J Radiat Biol 20:1–7. https://doi.org/10.1080/09553002.2022.2087932


Martínez SE, Cruz CC, Spinoso CJ, Bello-Bello JJ (2021) In vitro response of Vanilla (Vanilla planifolia Jacks. Ex Andrews) to PEG-induced osmotic stress. Sci Rep 11:22611. https://doi.org/10.1038/s41598-021-02207-0


Moreno BL, Reyes M, Rodríguez M, Kosky RG, Berkis R, Chong PB (2017) Respuesta De Cultivares De Musa spp. Al estrés hídrico in vitro inducido con polietilenglicol 6000. Rev Colomb Biotecnol 19:75–85. https://doi.org/10.15446/rev.colomb.biote.v19n2.60405


Predieri S (2001) Mutation induction and tissue culture in improving fruits. Plant Cell Tissue Organ Cult 64:185–210


Rai MK, Kalia RK, Singh R, Gangola MP, Dhawan AK (2011) Developing stress tolerant plants though in vitro selection- An overview of the recent progress. Environ Exp Bot 71:89–98. https://doi.org/10.1016/j.envexpbot.2010.10.021


Ramírez MM, Iglesias-Andreu LG, Enrique FV, Teixeira JA, Leyva OO, Murguía GJ (2019) Morphogenetic stability of variegated Vanilla planifolia jacks. Plants micropropagated in a temporary immersion system (TIB) rend. Lincei Sci Sci 30:603–609. https://doi.org/10.1007/s12210-019-00813-9


Ramos CA, Iglesias-Andreu LG, Bello-Bello JJ, Lee EH (2014) Improved propagation of Vanilla (Vanilla planifolia Jacks. Ex. Andrews) using a temporary immersion system. vitro Cell Dev Biol Plant 50:576–581. https://doi.org/10.1007/s11627-014-9602-8


Saepudin A, Khumaida N, Sopandie D, Wahyuning S (2017) In vitro selection of four soybean genotypes using PEG for drought tolerance. J Agron Indones 45:14–22. https://doi.org/10.24831/jai.v45i1.13749


Sánchez MS, Becerril AE, Tijerina L, Santizo JA (2001) Crecimiento Y desarrollo de vainilla en tres sistemas de producción en Papantla. Veracruz Rev Fitotec 24:49–56


Shabir H, Parvez A, Gosal S, Singh N (2010) In vitro screening of rice (Oryza sativa L) callus for drought tolerance. IJAB 5:108–115


Sorkheh K, Shiran B, Kbodambshi M, Rouhi V, Ercisli S (2011) In vitro assay of native Iranian almond species (Prunus L. spp.) for drought tolerance. PCTOC (Plant Cell, tissue and Organ Culture. 10:395–404. https://doi.org/10.1007/s11240-010-98791


Szarek S (2005) Use of concept of hormesis phenomenon to explain the law of diminishing returns. Part II. EJPAU Serie Econ 8:1–61


Villarreal ML, Herrera CB (2018) Requerimiento hídrico en el sistema de producción vainilla (Vanilla planifolia Jacks. Ex Andrews)- Naranjo (Citrus sinensis L.) en la región del Totonacapan, Veracruz, México. Agroproductividad, 11: 29–36




 


Acknowledgements



Author Information


Ramos-Castellá Alma Laura
Instituto de Biotecnología y Ecología Aplicada (INBIOTECA), Campus para la Cultura, las Artes y el Deporte, Universidad Veracruzana, Xalapa, México

Iglesias-Andreu Lourdes Georgina
Instituto de Biotecnología y Ecología Aplicada (INBIOTECA), Campus para la Cultura, las Artes y el Deporte, Universidad Veracruzana, Xalapa, México
liglesias@uv.mx

de Lourdes Palafox-Chávez María
Instituto Estatal de Oncología “Dr. Miguel Dorantes Mesa”, Xalapa, México