Exploring the potential of hydrogen sulfide in acquisition of aluminium stress tolerance in cyanobacterium Anabaena sp. PCC 7120

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Doi: 10.1007/s42535-023-00774-3
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Keywords: Antioxidants, Cyanobacteria, Heavy metals, Oxidative stress biomarkers, PS II photochemistry, Signaling


Abstract


Hydrogen sulfide (H2S), a gasotransmitter, has numerous roles in mitigation of stress in plants. Aluminium (Al) is found in acidic soil where it affects crop yields and their beneficial companion cyanobacteria. In the recent years, the fate of H2S in modulation of metal stress in plants has increasingly been reported, however, its role in cyanobacteria remains elusive. The present study pointed that excessive Al accumulation adversely affects the growth of the cyanobacterium Anabaena sp. PCC 7120 by declining pigments contents, rate of photosynthesis (oxygen evolution and PS II photochemistry), the level of antioxidants (non-enzymatic) and exopolysaccharides (EPS) secretion. Accumulation of Al in cells resulted in enhanced levels of reactive oxygen species (ROS: O2•− and H2O2) inside the cell, resulting severe cellular damage (MDA accumulation) despite of enhanced activities of enzymatic antioxidants. Exogenous hydrogen sulfide (NaHS, a donor of H2S) significantly reversed the negative effects of Al on the growth and growth promoting processes in the test organism by significantly lowering the accumulation of Al and oxidative stress biomarkers (O2•−, H2O2 and MDA) in cells. Reverse to this, the addition of hypotaurin (HT, a H2S scavenger) as well as DL- propargylglycine (PAG, an inhibitor of H2S generating enzyme L-cysteine desulfhydrase) the cells showed excessive accumulation of Al hence resulted enhanced oxidative biomarkers following considerable decline in antioxidant system. Furthermore, H2S depletion in cells caused considerable decline in EPS secretion which could be correlated with the increased accumulation of Al. Therefore, overall results pinpoint towards the role of endogenous H2S in alleviating the Al toxicity in Anabaena sp. PCC 7120.


Antioxidants, Cyanobacteria, Heavy metals, Oxidative stress biomarkers, PS II photochemistry, Signaling


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Acknowledgements


Authors are grateful to the University Grants Commission, New Delhi for providing chemical grant and Department of Botany, University of Allahabad, Prayagraj for providing necessary lab facilities to succeed the present work.


Author Information


Verma Nidhi
Ranjan Plant Physiology and Biochemistry Laboratory, Department of Botany, University of Allahabad, Prayagraj, India
nidhi111verma@gmail.com