Vegetos
(An International Journal of Plant Research & Biotechnology)
(eISSN: 2229-4473)
Ethnobotanical survey of medicinal plants used for oral health in Osogbo, Osun State, Nigeria
Research Articles | Published: 19 March, 2025
First Page: 1975
Last Page: 1986
Views: 30
Keywords: Antimicrobial activities, Ethnobotany, Indigenous knowledge, Oral health, Osogbo
Abstract
In Nigeria, the use of herbal medicines for oral health is prevalent because of their affordability, accessibility and efficacy. In this study, an ethnobotanical survey was conducted to record and analyze the medicinal plants used for oral health in Osogbo. Sixty-seven informants were interviewed for the local names of plants used, the plant parts, and how they are used. A literature review was conducted to assess the scientific validation of the recorded plants through antimicrobial studies on oral pathogens. Thirty-six species from 21 families were recorded from the ethnobotanical survey; families Euphorbiaceae and Fabaceae have the highest species representations, with five species each. Massularia acuminata is the most used plant, with FC, RFC and FL of 64, 0.99 and 99%, respectively. About 84% of the recorded plants were woody species. Chewing was the most preferred method of use, and twigs were the most used plant part. A literature review on the antimicrobial activities of the recorded plants revealed that extracts from 20 of the 36 implicated plants had been tested on oral pathogens, such as Porphyromonas gingivalis, Staphylococcus aureus, Streptococcus mutans, etc., thus confirming the folkloric usages of some of the recorded plants in oral health and care.
References
AbouZid SF, Mohamed AA (2011) Survey on medicinal plants and spices used in Beni-Sueif, upper Egypt. J Ethnobiol Ethnomed 7:1–6
Adegoke C, Sojobi A (2015) Climate change impact on infrastructure in Osogbo Metropolis, south-west Nigeria. J Emerg Trends Eng Appl Sci 6:156–165
Adeleye OA, Bamiro O, Akpotu M, Adebowale M, Daodu J, Sodeinde MA (2021) Physicochemical evaluation and antibacterial activity of massularia acuminata herbal toothpaste. Turk J Pharm Sci 18(4):476–482
Adenike AO (2021) Assessment of medicinal value of (G. Don) ex Hoyle (Massularia acuminata) stem as a potent Chew stick using GC-MS analysis. Br J Med Health Sci (BJMHS) 3(6):1068–1070
Adeniyi AA, Sofola OO, Kalliecharan RV (2012) An appraisal of the oral health care system in Nigeria. Int Dent J 62:292–300
Agbelade AD, Adeagbo DO (2020) Relationship between aboveground biomass and tree species diversity in two protected forests. J Res Manag 17:13–25
Agbulu C, Aboje E, Akande T (2015) Antimicrobial activity of Zanthoxylum zanthoxyloides root used as chewing stick (in Nigeria) on oral pathogens. Int Sci Res J 1:75–81
Ahonkhai I, Ayinde B, Edogun O, Uhuwmangho M (2009) Antimicrobial activities of the volatile oils of Ocimum bacilicum L. and Ocimum gratissimum L.(Lamiaceae) against some aerobic dental isolates. Pak J Pharm Sci 22(4):405
Ajao AA, Mukaila YO, Kenkpen DY (2023) An ethnobotanical study of medicinal plants used to treat and manage diabetes mellitus in Ede, Osun state Nigeria. Ethnobot Res Appl 25:1–18
Akinyele B, Oladejo B, Akinyemi A, Ezem L (2014) Comparative study of the antibacterial effect of mouth washes and Vernonia amygdalina (Del.) on some tooth decay causing bacteria. Br Microbiol Res J 4(7):749–758
Amtaghri S, Akdad M, Slaoui M, Eddouks M (2022) Traditional uses, Pharmacological, and phytochemical studies of euphorbia: A review. Curr Top Med Chem 22(19):1553–1570
Anand G, Ravinanthan M, Basaviah R, Shetty AV (2015) In vitro antimicrobial and cytotoxic effects of Anacardium occidentale and Mangifera indica in oral care. J Pharm Bioallied Sci 7:69–74
Anibijuwon I, Oladejo B, Adetitun D, Kolawole O (2012) Antimicrobial activities of Vernonia amygdalina against oral microbes. Glob J Pharmacol 6(3):178–185
Anyanwu OO, Eze PM, Nnaoma EI, Ngwoke KG (2018) Antimicrobial properties of Jatropha curcas L. against dental pathogens. Glob J Med Res 18:13–18
Arshad MS, Hussain I, Mahmood MS, Khan MN (2017) Evaluation of antimicrobial potential of Acacia nilotica (kikar) against oral pathogens associated with caries and periodontitis. Pak J Agric Sci 54(2):423–430
Awoyemi O, Abdulkarim I, Ewa E, Aduloju A (2012) Ethnobotanical assessment of herbal plants in South-Western Nigeria. Acad Res Int 2(3):50–57
Bashiru BO, Omotunde SM (2014) Burden of oral diseases and dental treatment needs of an urban population in Port Harcourt, rivers State, Nigeria. Eur J Gen Dent 3:125–128
Burkill HM (2004) The useful plants of West tropical Africa, general index. Royal Botanic Gardens, Kew
Chan WSH (2024) The role of oral health in the prevention of systemic diseases. Univers Lib Med Health Sci 1(1)
Cordeiro TO, de Mendonça AKP, Gomes MS, de Lima ELF, Costa MRM, de Lima KC, Lins RDAU (2021) Antimicrobial action of the hydroethanolic extract of Spondias mombin L. against oral bacteria of the genus Streptoc occus. Res Soc Dev 10(1):e39310111539
Costa KCS, Bezerra SB, Norte CM, Nunes LMN, Olinda TM (2012) Medicinal plants with teratogenic potential: current considerations. Braz J Pharm Sci 48:427–433
Darout IA, Åstrøm AN, Skaug N (2005) Knowledge and behaviour related to oral health among secondary school students in Khartoum Province, Sudan. Int Dent J 55:224–230
Dayok O, Dawang D, Da’am C (2018) Antimicrobial activity of leaf extract of Anogeissus Leiocarpus (African Birch) on some selected clinical isolates. J Pharm Biol Sci 13:36–40
Dilbato T, Begna F, Tolosa T (2023) Medicinal plants and non-plant remedies used in the treatment of livestock ailments in Dawuro zone, Southwestern Ethiopia. Ethiop Vet J 27(1):72–92
Erhunse N, Omoregie ES, Sahal D (2023) Antiplasmodial and antimalarial evaluation of a Nigerian hepta-herbal Agbo-iba Decoction: identification of magic bullets and possible facilitators of drug action. J Ethnopharmacol 301:115807
Erinoso SM, Aworinde DO (2018) Current outlook and future promise of ethnobotany in Nigeria: A review and personal observation. Afr J Plant Sci 12:73–80
Gao Y, Dong Y, Guo Q, Wang H, Feng M, Yan Z, Bai D (2022) Study on supramolecules in traditional Chinese medicine Decoction. Molecules 27(10):3268
Gomashe AV, Sharma AA, Kasulkar A (2014) Investigation of biofilm Inhibition activity and antibacterial activity of Psidium guajava plant extracts against Streptococcus mutans causing dental plaque. Int J Curr Microbiol Appl Sci 3:335–351
Hioki Y, Onwona-Agyeman S, Kakumu Y, Hattori H, Yamauchi K, Mitsunaga T (2020) Garcinoic acids and a benzophenone derivative from the seeds of Garcinia kola and their antibacterial activities against oral bacterial pathogenic organisms. J Nat Prod 83:2087–2092
Hussain KA, Tarakji B, Kandy BPP, John J, Mathews J, Ramphul V, Divakar DD (2015) Antimicrobial effects of Citrus sinensis Peel extracts against periodontopathic bacteria: an in vitro study. Rocz Panstw Zakl Hig 66(2):173–178
Hutchinson J, Dalziel JM (1972) Flora of West Tropical Africa, (Second edition), Volume 3, Parts 1 & 2 [Hepper, F.N. (Ed.)]. Crown Agents for Overseas Governments and Administrations, London UK, pp 574
Idu M, Umweni A, Odaro T, Ojelede L (2009) Ethnobotanical plants used for oral healthcare among the Esan tribe of Edo State, Nigeria. Ethnobot Leafl 13:548–563
Igunma OS, Azodo CC (2020) The effect of chewing stick use on oral hygiene and gingival health of young adults in Nigeria. Niger J Dent Res 5:155–160
Islam MS, Ara H, Ahmad KI, Uddin MM (2019) A review on medicinal uses of different plants of Euphorbiaceae family. Univers J Pharm Res 4(1):45–49
Iyiola AO, Adegoke Wahab MK (2024) Herbal medicine methods and practices in Nigeria. In: Izah SC, Ogwu MC, Akram M (eds) Herbal medicine phytochemistry: applications and trends. Springer International Publishing, UK, pp 1395–1428
Jana?kovi? P, Gavrilovi? M, Mileti? M, Radulovi? M, Kolašinac S, Stevanovi? ZD (2022) Small regions as key sources of traditional knowledge: a quantitative ethnobotanical survey in the central Balkans. J Ethnobiol Ethnomed 18(1):70
Jeffrey J, Satari MH, Kurnia D, Sudigdoadi S (2020) Inhibition of Streptococcus mutans growth induced by the extract of Citrus aurantifolia Peel. J Int Dent Med Res 13:122–127
John D, Okwubie L, Oyakhire EJ (2022) Evaluation of the antibacterial activities of methanol stem extract of massularia acuminata and methanol twig extract of Garcinia kola on oral Bacteria and their effect in combination. GSC Biol Pharm Sci 18:118–128
Kareem KT, Oluwatoyin AS, Olabisi AA, Rashidat EA, Oluwatoyin AT (2012) Antibacterial effect of distemonanthus Benthamianus extract against some oral pathogens. Int J Appl Sci Technol 2(2):114–119
Kayode J, Omotoyinbo MA (2008) Conservation of botanicals used for dental and oral healthcare in Ekiti State, Nigeria. Ethnobot Leafl 12:7–18
Khijmatgar S, Belur G, Venkataram R, Karobari MI, Marya A, Shetty V, Chowdhury A, Gootveld M, Lynch E, Shetty S (2021) Oral candidal load and oral health status in chronic obstructive pulmonary disease (COPD) patients: a case-cohort study. BioMed Res Int 5548746. https://doi.org/10.1155/2021/5548746
Khot AJP, Ankola AV, Dodamani S, Sankeshwari RM, Kumar RS, Santhosh VN (2023) Assessment of potential antimicrobial activity of Ocimum basilicum extract and chlorhexidine against Socransky’s complex pathogens of oral cavity: an in vitro study. J Indian Soc Periodontol 27:479–486
Kinasih LK, Nabahan I, Erawati S, Simanjuntak RNM (2021) Effectivity of Jatropha multifida L. leaves extract as antibacterial on Streptococcus mutans using in vitro testing methods. Biomed J Indones 7:415–421
Lekshmi N, Sowmia N, Viveka S, Brindha JR, Jeeva S (2012) The inhibiting effect of Azadirachta indica against dental pathogens. Asian J Plant Sci Res 2:6–10
Lemes RS, Alves CC, Estevam EB, Santiago MB, Martins CH, SANTOS TCD, Crotti AE, Miranda ML (2018) Chemical composition and antibacterial activity of essential oils from Citrus aurantifolia leaves and fruit Peel against oral pathogenic bacteria. Acad Bras 90:1285–1292
Leonti M (2022) The relevance of quantitative ethnobotanical indices for ethnopharmacology and ethnobotany. J Ethnopharmacol 288:115008
Makgobole MU, Onwubu SC, Nxumal CT, Mpofana N, Ajao AA (2023) In search of oral cosmetics from nature: A review of medicinal plants for dental care in West Africa. S Afr J Bot 162:644–657
Mercy O, Enabulele Joan E (2017) Herbal/Traditional medicine use and self-medication among patients prior to seeking oral health care in a tertiary health facility in Nigeria. J Med Res 3:127–131
Millones-Gómez PA, Maurtua-Torres D, Bacilio-Amaranto R, Calla-Poma RD, Requena-Mendizabal MF, Valderrama-Negron AC, Calderon-Miranda MA, Calla-Poma RA, Huauya-Leuyacc ME (2020) Antimicrobial activity and antiadherent effect of Peruvian Psidium guajava (Guava) leaves on a cariogenic biofilm model. J Contemp Dent Pract 21:733–740
Moghadam S, Azari B, Rashidi R, Bafghi MH, Rakhshandeh H, Selman SM, Zarrinfar H (2023) Antifungal activity of three different varieties of Capsicum annuum against clinical isolates of Candida species. Trop Dis Travel Med Vaccin 9:9
Muddathir AM, Mohieldin EAM, Mitsunaga T (2020) In vitro activities of Acacia nilotica (L.) delile bark fractions against oral bacteria, glucosyltransferase and as antioxidant. BMC Complement Med Ther 20:1–9
Mukaila YO, Oladipo OT, Arogundade OO, Ajao AAN (2022) Traditional knowledge of medicinal plants used in Ile-Ife, Southwestern Nigeria. Asian J Ethnobiol 5(2)
Namadina M, Mukhtar A, Karaye S, Musa F, Bah I, Maitama F (2021) Phytochemical constituents and antibacterial activities of Indigenous chewing stick (Anogeissus leiocarpus) stem. Bayero J Pure Appl Sci 14:85–94
Ndhlovu PT, Asong JA, Omotayo AO, Otang-Mbeng W, Aremu AO (2023) Ethnobotanical survey of medicinal plants used by Indigenous knowledge holders to manage healthcare needs in children. PLoS ONE 18:e0282113
National Population Commission (NPC) (2006) Nigeria 2006 census figures. National Population Commission, Nigeria
Nwaiwu SO, Adekanmbi H, Igbari D, Oyejide SO, Nwaiwu O, Amao O (2022) Antimicrobial activities and phytochemical screening of five Nigerian chewing sticks. Int J Sci Res Biol Sci 9(4):10–13
Nwankwo OS, Chioma CG, Ofem OW, Amara EM, Chijioke MC (2017) Aqueous methanol extract of the root bark of Zanthoxylum zanthoxyloides provides natural remedy for dental caries and toothache. World J Pharm Res 6(7):336–349
Nwaokorie F, Coker A, Ogunsola F, Oyedele G, Ayanbadejo P, Abdurrazaq T, Umezudike A (2010) Antimicrobial activities of Garcinia kola on oral Fusobacterium nucleatum and biofilm. Afr J Microbiol Res 4(7):509–514
Obistioiu D, Cocan I, Tîrziu E, Herman V, Negrea M, Cucerzan A, Neacsu AG, Cozma AL, Nichita I, Hulea A, Radulov I (2021) Phytochemical profile and Microbiological activity of some plants belonging to the Fabaceae family. Antibiotics 10(6):662
Ofeimun J, Babaiwa U, Erharuyi O, Blessed B, Eze A (2022) Antimicrobial and antioxidant activities of volatile oils from four citrus species on selected oral pathogens and GC-MS identification of associated compounds. J Sci Technol(Ghana) 40:40–55
Ogunlakina A, Sonibare M (2019) Ethnobotanical survey of medicinal plants used as remedy for female infertility and menstrual disorder in Southwestern Nigeria. Niger J Pharm Res 15:205–217
Ogunsola O, Egbewale S (2018) Factors influencing the use of herbs and combination with orthodox medicine for healthcare management in Ibadan, Nigeria. World News Nat Sci 17:39–47
Ökmen G, Mammadhkanl? M, Vurkun M (2018) The antibacterial activities of Syzygium aromaticum (L.) Merr. & Perry against oral bacteria and its antioxidant and antimutagenic activities. Int J Pharm Sci Res 9(11):4634–4641
Olusola AO, Bakare OS, Ekun OE, Olusola AO (2020) A comparative study on the effect of Massularia acuminata fractions against bacterial and fungal isolates from the oral cavity. Health 12(7):101879
Oshomoh E, Idu M (2012) Phytochemical screening and antimicrobial activity of ethanol and aqueous extracts of stem of Glyphaea brevis (spreng.) Monachino on oral microorganisms. Pharmacogn J 4:23–30
Osun State Government (2023) Major Towns in Osun State. Available via https://www.osunstate.gov.ng/about/major-towns/osogbo/. Accessed 14 February 2024
Osuh ME, Oke GA, Lilford RJ, Owoaje E, Harris B, Taiwo OJ, Yeboah G, Abiona T, Watson SI, Hemming K, Quinn L (2022) Prevalence and determinants of oral health conditions and treatment needs among slum and non-slum urban residents: evidence from Nigeria. PLOS Glob Public Health 2(4):pe0000297
Peres MA, Macpherson LM, Weyant RJ, Daly B, Venturelli R, Mathur MR, Listl S, Celeste RK, Guarnizo-Herreño CC, Kearns C (2019) Oral diseases: a global public health challenge. Lancet 394:249–260
Saggar S, Mir PA, Kumar N, Chawla A, Uppal J, Kaur A (2022) Traditional and herbal medicines: opportunities and challenges. Pharmacogn Res 14(2)
Sakpere A, Oladipo O, Mukaila Y, Ayinde J, Oluwaniyi O (2022) Indigenous leafy vegetables and health management in south-western Nigeria: A review based on Osun state. In: Babalola OO, Ayangbenro AS, Ojuederie OB (eds) Food security and safety volume 2: African perspectives. Springer, New York, pp 115–134
Salman HA, Senthilkumar R, Vasundhara M (2018) Lack of antibacterial activity of Capsicum annuum and Simarouba glauca against Streptococcus mutans and Streptococcus sobrinus. Biosci Biotechnol Res Asia 15(2):311–315
Tardío J, Pardo-de-Santayana M (2008) Cultural importance indices: a comparative analysis based on the useful wild plants of Southern Cantabria (Northern Spain). Econ Bot 62:24–39
Tobin AO, Ajayi IO (2017) Common oral conditions and correlates: an oral health survey in Kwara state Nigeria. BMC Res Notes 10:1–9
Trotter RT, Logan MH (1986) Informant consensus: a new approach for identifying potentially effective medicinal plants. In: Etkin NL (ed) Plants in Indigenous medicine and diet, behavioural approaches. Redgrave Publishing Company, New York, pp 91–112
Usman M, Khan WR, Yousaf N, Akram S, Murtaza G, Kudus KA, Ditta A, Rosli Z, Rajpar MN, Nazre M (2022) Exploring the phytochemicals and anti-cancer potential of the members of Fabaceae family: A comprehensive review. Molecules 27(12):3863
Van Wyk BE (2019) The diversity and multiple uses of Southern African legumes. Aust Syst Bot 32(6):519–546
Velu G, Palanichamy V, Rajan AP (2018) Phytochemical and Pharmacological importance of plant secondary metabolites in modern medicine. In: Roopan SM, Madhumitha G (eds) Bioorganic phase in natural food: an overview. Springer, New York, pp 135–156
Wang Y, Chen S, Du K, Liang C, Wang S, Boadi EO, Li J, Pang X, He J, Chang Y-x (2021) Traditional herbal medicine: therapeutic potential in rheumatoid arthritis. J Ethnopharmacol 279:114368
World Health Organization (WHO) (2022) Oral health. Available via https://www.who.int/news-room/fact-sheets/detail/oral-health. Accessed 5 February 2025
World Health Organization (WHO) (2023) Traditional Medicine. Available via https://www.who.int/news-room/questions-and-answers/item/traditional-medicine. Accessed 14 February 2024
Zhang Y, Wang Y, Zhu X, Cao P, Wei S, Lu Y (2017) Antibacterial and antibiofilm activities of Eugenol from essential oil of Syzygium aromaticum (L.) Merr. & LM Perry (clove) leaf against periodontal pathogen Porphyromonas gingivalis. Microb Pathog 113:396–402
Author Information
Department of Botany and Plant Biotechnology, University of Johannesburg, Johannesburg, South Africa