Effect of modified MS medium on in vitro propagation of Indigofera barberi Gamble An endemic hepatoprotective, recalcitrant medicinal plant of Seshachalam Biosphere reserve

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Doi: 10.1007/s42535-023-00741-y
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Keywords: n Indigofera barberin , Culture media, Plant growth regulators, In vitro culture


Abstract


Indigofera barberi belongs to Fabaceae is an endangered, high valued endemic erect, perennial under shrub. Therefore standardization of micropropagation protocol is desirable for its ex situ conservation. The present study describes micropropagation protocol for I. barberi using nodal explants. Three culture media (MS, B5 and WPM) tested for shoot initiation. MS medium was chosen based on better bud break and shoot initiation. Further, different strengths of MS major salts tried and 100% of shoot initiation observed on MS (major – HS) containing BAP/ Zeatin (0.5 mg/L) /Adenine sulphate (25 mg/L). Maximum shoot multiplication rate (17.4 ± 0.01) and shoot length (2.10 ± 0.18 cm) attained by using MS (major – HS (Half strength)) with 0.5 mg/L each BAP, KN and AgNO3. Rhizogenesis of regenerated shoots successfully achieved on ½ strength MS with 1.0 mg/L IBA with root number (8.2 ± 0.44) and length (6.73 ± 0.26 cm). Hardening and acclimatization was successful with 80% of survival rate of plantlets.


n              Indigofera barberin            , Culture media, Plant growth regulators,  In vitro culture


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Acknowledgements



Author Information


Reddy G. Sindhu
Department of Biotechnology, Sri Venkateswara University, Tirupati, India

Saritha K. V.
Department of Biotechnology, Sri Venkateswara University, Tirupati, India
kvsarithasvu@gmail.com